Reviewed, UniProtKB/Swiss-Prot Q88QZ4 (GLNE_PSEPK)

Names and origin

Protein names

Recommended name:
    Glutamate-ammonia-ligase adenylyltransferase
    EC=2.7.7.42
Alternative name(s):
    [Glutamate--ammonia-ligase] adenylyltransferase
    Glutamine-synthetase adenylyltransferase
      Short name=ATase

Gene names

Name:	glnE
Ordered Locus Names:	PP_0340

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	977 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adenylation and deadenylation of glutamine synthetase By similarity.
Catalytic activity	ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. HAMAP MF_00802
Sequence similarities	Belongs to the glnE family.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [glutamate-ammonia-ligase] adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 977	977	Glutamate-ammonia-ligase adenylyltransferase HAMAP MF_00802		PRO_0000209268
Regions
	Region	108 – 319	212	GlnE 1 HAMAP MF_00802
	Region	628 – 850	223	GlnE 2 HAMAP MF_00802

Sequences

	Sequence		Length	Mass (Da)
	Q88QZ4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1159DA2BC1589296	FASTA	977	109,433
	10 20 30 40 50 60 MRLPLPSDLP ATLQPLVTRN QQFISDAVAG HPELDLQAWS PLHRQQFDQV AAASEFVLSL 70 80 90 100 110 120 AQREPAMLFA LLASGELERR YAPGELRGQI AATAQAAQSE DELARNLRRA RNRQQLRIIW 130 140 150 160 170 180 RDITRQAELG ETCRDLSDLA DAAIDEAYQW LYPRHCQQFG TPIGNRSGQP QHMVVLGMGK 190 200 210 220 230 240 LGAVELNLSS DIDLIFGFPE GGETEGVKRS LDNQEFFTRL GQRLIKALDP VTVDGFVFRV 250 260 270 280 290 300 DMRLRPYGSA GALVLSFNAL EQYYQDQGRD WERYAMIKAR VVAGDQAAGA QLQEMLRPFV 310 320 330 340 350 360 YRRYLDFSAI EALRTMKQLI QQEVRRKGMA ENIKLGAGGI REVEFIAQAF QLIHGGRDLS 370 380 390 400 410 420 LQQRPLLKVL ATLEGQGYLP PAVVAELREG YEFLRYTEHA IQAIADRQTQ MLPEGETDQA 430 440 450 460 470 480 RVAYVLGFAD WQSFHDQLMY WRGRIDWHFR QVIADPDDED GEGELVVGGE WSPLWEQAQD 490 500 510 520 530 540 EEAAGRQLQE AGFKQPAEAL RRLAGLRSSP QLRSMQRIGR ERLDAFIPRL LAQAVEHDNP 550 560 570 580 590 600 DLVLERVLPL VEAVARRSAY LVLLTENPGA LRRLLTLCAA SPWIAEQIAL YPLLLDELLN 610 620 630 640 650 660 EGRLFSPPLA PELASELRER LTRIPEDDLE QQMEALRHFK LAHSLRVAAS EISGNLPLMK 670 680 690 700 710 720 VSDYLTWLAE AILDQVLALA WRQTVARHGQ PKRSDGSLCD PGFIIIGYGK MGGLELGHGS 730 740 750 760 770 780 DLDLVFIHDG DPQAETDGAK PIDSAQFFTR LGQRIIHLLT TQTNSGQLYD VDMRLRPSGA 790 800 810 820 830 840 SGLLVSSLGA FERYQQNEAW TWEHQALVRA RVLVGCKQVG AAFEGVRAKV LGQARDLEKL 850 860 870 880 890 900 RGEVSEMRAK MRDNLGTKAT AAGTAANAFD AGVPFDIKQD AGGIVDIEFM VQYAALAWSH 910 920 930 940 950 960 DHPAILRWTD NIRILEELEQ ANLMPASDAV LLREVYKAFR SASHRQALQK EAGVIDAAQF 970 ADERREVRRI WGELGLS

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN65971.1.
RefSeq	NP_742507.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044045.
GenomeReviews	Gene locus PP_0340 in contig AE015451_GR.
KEGG	ppu:PP_0340.
NMPDR	fig|160488.1.peg.337.
TIGR	PP_0340.
Phylogenomic databases
HOGENOM	Q88QZ4.
OMA	Q88QZ4. WERYAMI.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0340-MON.
Family and domain databases
HAMAP	MF_00802. [Tree]
InterPro	IPR005190. GlnE. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
Pfam	PF08335. GlnD_UR_UTase. 1 hit. PF03710. GlnE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNE_PSEPK

Accession

Primary (citable) accession number: Q88QZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)