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Q88QX8

- MASZ_PSEPK

UniProt

Q88QX8 - MASZ_PSEPK

Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (30 Apr 2003)
      Previous versions | rss
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    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei276 – 2761Acetyl-CoAUniRule annotation
    Binding sitei313 – 3131Acetyl-CoAUniRule annotation
    Active sitei340 – 3401Proton acceptorUniRule annotation
    Binding sitei340 – 3401GlyoxylateUniRule annotation
    Metal bindingi432 – 4321MagnesiumUniRule annotation
    Binding sitei432 – 4321GlyoxylateUniRule annotation
    Metal bindingi460 – 4601MagnesiumUniRule annotation
    Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei631 – 6311Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciPPUT160488:GIXO-366-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:PP_0356
    OrganismiPseudomonas putida (strain KT2440)
    Taxonomic identifieri160488 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
    ProteomesiUP000000556: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 725725Malate synthase GPRO_0000166893Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi160488.PP_0356.

    Structurei

    3D structure databases

    ProteinModelPortaliQ88QX8.
    SMRiQ88QX8. Positions 2-722.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
    Regioni457 – 4604Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSQFIENE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q88QX8-1 [UniParc]FASTAAdd to Basket

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    MTGYVQVGGL QVAKVLYDFV NNEAIPGTGI VAEQFWAGAE KIINDLAPKN    50
    KALLAKRDEL QAKIDAWHQA RKGQAHDAAA YKAFLQEIGY LLPQADDFQA 100
    TTQNVDEEIA HMAGPQLVVP VMNARFALNA ANARWGSLYD ALYGTDAISD 150
    EGGAEKGQGY NKVRGDKVIA FARAFLDEAA PLAAGSHVDS TGYRIEGGKL 200
    VVALKGGSNT GLRDDAQLIG FHGDAAAPTA VLLKHNGLHF EIQVDASTPV 250
    GSTDAAGVKD ILMESALTTI MDCEDSVAAV DADDKVIVYR NWLGLMKGDL 300
    AESVSKGGKT FTRTMNPDRE YAAPNGGSVT LHGRSLLFVR NVGHLMTNPA 350
    ILDAQGNEIP EGIQDGLFTN LIALHNLNGN TSRKNTRSGS VYIVKPKMHG 400
    PEEVAFATEI FSQVEDLLGM PRNTVKVGIM DEERRTTVNL KSCIKAAAER 450
    VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKNEKWIGAY ENNNVDVGLA 500
    TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAATLHA 550
    LHYHKVDVQA RQRELASRTP ASVDDILAIP LAADTNWSAE EIRNELDNNA 600
    QGILGYVVRW IDQGVGCSKV PDINNVGLME DRATLRISAQ LLANWLRHGV 650
    VSQEQVLESL KRMAVVVDQQ NAGDPLYRPM APNFDDNVAF QAAVELVVEG 700
    GKQPNGYTEP VLHRRRREFK ARNGL 725
    Length:725
    Mass (Da):78,347
    Last modified:April 30, 2003 - v1
    Checksum:i8363F218E6116AE1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015451 Genomic DNA. Translation: AAN65987.1.
    RefSeqiNP_742523.1. NC_002947.3.

    Genome annotation databases

    EnsemblBacteriaiAAN65987; AAN65987; PP_0356.
    GeneIDi1044063.
    KEGGippu:PP_0356.
    PATRICi19938751. VBIPsePut30601_0384.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AE015451 Genomic DNA. Translation: AAN65987.1 .
    RefSeqi NP_742523.1. NC_002947.3.

    3D structure databases

    ProteinModelPortali Q88QX8.
    SMRi Q88QX8. Positions 2-722.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 160488.PP_0356.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAN65987 ; AAN65987 ; PP_0356 .
    GeneIDi 1044063.
    KEGGi ppu:PP_0356.
    PATRICi 19938751. VBIPsePut30601_0384.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi SQFIENE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci PPUT160488:GIXO-366-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: KT2440.

    Entry informationi

    Entry nameiMASZ_PSEPK
    AccessioniPrimary (citable) accession number: Q88QX8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3