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Q88QX8

- MASZ_PSEPK

UniProt

Q88QX8 - MASZ_PSEPK

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Protein

Malate synthase G

Gene

glcB

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei118 – 1181Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei276 – 2761Acetyl-CoAUniRule annotation
Binding sitei313 – 3131Acetyl-CoAUniRule annotation
Active sitei340 – 3401Proton acceptorUniRule annotation
Binding sitei340 – 3401GlyoxylateUniRule annotation
Metal bindingi432 – 4321MagnesiumUniRule annotation
Binding sitei432 – 4321GlyoxylateUniRule annotation
Metal bindingi460 – 4601MagnesiumUniRule annotation
Binding sitei541 – 5411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei631 – 6311Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-366-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:PP_0356
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 725725Malate synthase GPRO_0000166893Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei617 – 6171Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_0356.

Structurei

3D structure databases

ProteinModelPortaliQ88QX8.
SMRiQ88QX8. Positions 2-722.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1262Acetyl-CoA bindingUniRule annotation
Regioni457 – 4604Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OMAiSQFIENE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88QX8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTGYVQVGGL QVAKVLYDFV NNEAIPGTGI VAEQFWAGAE KIINDLAPKN
60 70 80 90 100
KALLAKRDEL QAKIDAWHQA RKGQAHDAAA YKAFLQEIGY LLPQADDFQA
110 120 130 140 150
TTQNVDEEIA HMAGPQLVVP VMNARFALNA ANARWGSLYD ALYGTDAISD
160 170 180 190 200
EGGAEKGQGY NKVRGDKVIA FARAFLDEAA PLAAGSHVDS TGYRIEGGKL
210 220 230 240 250
VVALKGGSNT GLRDDAQLIG FHGDAAAPTA VLLKHNGLHF EIQVDASTPV
260 270 280 290 300
GSTDAAGVKD ILMESALTTI MDCEDSVAAV DADDKVIVYR NWLGLMKGDL
310 320 330 340 350
AESVSKGGKT FTRTMNPDRE YAAPNGGSVT LHGRSLLFVR NVGHLMTNPA
360 370 380 390 400
ILDAQGNEIP EGIQDGLFTN LIALHNLNGN TSRKNTRSGS VYIVKPKMHG
410 420 430 440 450
PEEVAFATEI FSQVEDLLGM PRNTVKVGIM DEERRTTVNL KSCIKAAAER
460 470 480 490 500
VVFINTGFLD RTGDEIHTSM EAGAVVRKGA MKNEKWIGAY ENNNVDVGLA
510 520 530 540 550
TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV PSPTAATLHA
560 570 580 590 600
LHYHKVDVQA RQRELASRTP ASVDDILAIP LAADTNWSAE EIRNELDNNA
610 620 630 640 650
QGILGYVVRW IDQGVGCSKV PDINNVGLME DRATLRISAQ LLANWLRHGV
660 670 680 690 700
VSQEQVLESL KRMAVVVDQQ NAGDPLYRPM APNFDDNVAF QAAVELVVEG
710 720
GKQPNGYTEP VLHRRRREFK ARNGL
Length:725
Mass (Da):78,347
Last modified:April 30, 2003 - v1
Checksum:i8363F218E6116AE1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN65987.1.
RefSeqiNP_742523.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN65987; AAN65987; PP_0356.
GeneIDi1044063.
KEGGippu:PP_0356.
PATRICi19938751. VBIPsePut30601_0384.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN65987.1 .
RefSeqi NP_742523.1. NC_002947.3.

3D structure databases

ProteinModelPortali Q88QX8.
SMRi Q88QX8. Positions 2-722.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160488.PP_0356.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN65987 ; AAN65987 ; PP_0356 .
GeneIDi 1044063.
KEGGi ppu:PP_0356.
PATRICi 19938751. VBIPsePut30601_0384.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OMAi SQFIENE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci PPUT160488:GIXO-366-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiMASZ_PSEPK
AccessioniPrimary (citable) accession number: Q88QX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: October 29, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3