Reviewed, UniProtKB/Swiss-Prot Q88QX8 (MASZ_PSEPK)

Names and origin

Protein names

Recommended name:
    Malate synthase G
    EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	PP_0356

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	725 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 725	725	Malate synthase G HAMAP MF_00641		PRO_0000166893
Sites
	Active site	340	1	Proton acceptor By similarity
	Active site	631	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88QX8-1 [UniParc]. Last modified April 30, 2003. Version 1. Checksum: 8363F218E6116AE1	FASTA	725	78,347
	10 20 30 40 50 60 MTGYVQVGGL QVAKVLYDFV NNEAIPGTGI VAEQFWAGAE KIINDLAPKN KALLAKRDEL 70 80 90 100 110 120 QAKIDAWHQA RKGQAHDAAA YKAFLQEIGY LLPQADDFQA TTQNVDEEIA HMAGPQLVVP 130 140 150 160 170 180 VMNARFALNA ANARWGSLYD ALYGTDAISD EGGAEKGQGY NKVRGDKVIA FARAFLDEAA 190 200 210 220 230 240 PLAAGSHVDS TGYRIEGGKL VVALKGGSNT GLRDDAQLIG FHGDAAAPTA VLLKHNGLHF 250 260 270 280 290 300 EIQVDASTPV GSTDAAGVKD ILMESALTTI MDCEDSVAAV DADDKVIVYR NWLGLMKGDL 310 320 330 340 350 360 AESVSKGGKT FTRTMNPDRE YAAPNGGSVT LHGRSLLFVR NVGHLMTNPA ILDAQGNEIP 370 380 390 400 410 420 EGIQDGLFTN LIALHNLNGN TSRKNTRSGS VYIVKPKMHG PEEVAFATEI FSQVEDLLGM 430 440 450 460 470 480 PRNTVKVGIM DEERRTTVNL KSCIKAAAER VVFINTGFLD RTGDEIHTSM EAGAVVRKGA 490 500 510 520 530 540 MKNEKWIGAY ENNNVDVGLA TGLQGRAQIG KGMWAMPDLM AAMLEQKIAH PLAGANTAWV 550 560 570 580 590 600 PSPTAATLHA LHYHKVDVQA RQRELASRTP ASVDDILAIP LAADTNWSAE EIRNELDNNA 610 620 630 640 650 660 QGILGYVVRW IDQGVGCSKV PDINNVGLME DRATLRISAQ LLANWLRHGV VSQEQVLESL 670 680 690 700 710 720 KRMAVVVDQQ NAGDPLYRPM APNFDDNVAF QAAVELVVEG GKQPNGYTEP VLHRRRREFK ARNGL

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN65987.1.
RefSeq	NP_742523.1.
3D structure databases
HSSP	HSSP built from PDB template 1N8I based on UniProtKB Q50596.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88QX8.
Genome annotation databases
GeneID	1044063.
GenomeReviews	Gene locus PP_0356 in contig AE015451_GR.
KEGG	ppu:PP_0356.
NMPDR	fig|160488.1.peg.353.
TIGR	PP_0356.
Phylogenomic databases
HOGENOM	Q88QX8.
OMA	PKMHGPD.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0356-MON.
Family and domain databases
HAMAP	MF_00641. [Tree]
InterPro	IPR011076. Malate_synth-like_core. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_PSEPK

Accession

Primary (citable) accession number: Q88QX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	November 3, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)