Q88QV3 (PQQF_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Coenzyme PQQ synthesis protein F
EC=3.4.24.-

Alternative name(s):
Pyrroloquinoline quinone biosynthesis protein F

Gene names

Name:	pqqF
Ordered Locus Names:	PP_0381

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	766 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for coenzyme pyrroloquinoline quinone (PQQ) biosynthesis. It is thought that this protein is a protease that cleaves peptides bond in a small peptide (gene pqqA), providing the glutamate and tyrosine residues which are necessary for the synthesis of PQQ By similarity.
Cofactor	Binds 1 zinc ion per subunit By similarity.
Pathway	Cofactor biosynthesis; pyrroloquinoline quinone biosynthesis.
Sequence similarities	Belongs to the peptidase M16 family.

Ontologies

Keywords
Biological process	PQQ biosynthesis
Ligand	Metal-binding Zinc
Molecular function	Hydrolase Metalloprotease Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW pyrroloquinoline quinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: InterPro metalloendopeptidase activity Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 766	766	Coenzyme PQQ synthesis protein F		PRO_0000074413
Sites
	Active site	52	1	Proton acceptor By similarity
	Metal binding	49	1	Zinc By similarity
	Metal binding	53	1	Zinc By similarity
	Metal binding	130	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88QV3 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0AEDF15A8C6C4206	FASTA	766	84,242
	10 20 30 40 50 60 MPDAIRQLTL ANGLQLTLRH APRLKRSAAA LRVHAGSHDA PAKWPGLAHF LEHLFFLGTP 70 80 90 100 110 120 RFPLEDGLMR YVQALGGQVN ASTRERATDF FFEVPPNALG GGLERLCQML AEPDLGIERQ 130 140 150 160 170 180 RREREVIHAE FIAWSRNPTA QQQFALLQSV SARHPLGAFH AGNRYTLALH DAAFQQALAG 190 200 210 220 230 240 FHQRFYQGGQ ICLSLCGPQP LDELERLARQ QAELFAAGER VPQILPPPLP AMASALTFTH 250 260 270 280 290 300 QSLPSGAEHA LELLIAYLED SRPGTWLGAL RERGWLRRFT AERLYAFAGQ LLWHLDLKLS 310 320 330 340 350 360 ADACPDEASA LLQGWFRFIR QADREQLNHQ FGLLQHSRAH SASALELARR DSTGQPFAKL 370 380 390 400 410 420 DTQGLQALGA LLESLPGADH GDWQLLPVDP LLKADLPHAK AQPLPAALKI SDQLPPARQF 430 440 450 460 470 480 AALYLRWHVP SPMRQPLQRV LEQALAPLQE RCDRASVQLQ YSSAGEYWQL HCAGLPAAVL 490 500 510 520 530 540 RAVEQALALM LKPPASCWLP CTALPPALIP IRALLKQLPD AVRGSLPPAV PACTLTQQQL 550 560 570 580 590 600 DSLWLHTEWH GMAAGFEDTA LKALGAALEQ CPGQGSRPSP LPTWANHRWQ HAQVPGSEHA 610 620 630 640 650 660 LLLFCPLPAA KEAAGRLLAQ LLQGPVYQRL RVDLQLGYAV FSAFRQVEGV GGLLFGVQSP 670 680 690 700 710 720 HTDQAQVLDH LLNLLRHGVT LDPAARQALA GQFDEPAMAN ADVAEWAWQT HLATQADRLD 730 740 750 760 VLRRSILTTR QTDLDHLLSA LLDPGSAWLC LANAAAPDTS WQGENR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN66012.1.
RefSeq	NP_742548.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88QV3.
ModBase	Search...
Protein-protein interaction databases
STRING	160488.PP_0381.
Protein family/group databases
MEROPS	M16.006.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN66012; AAN66012; PP_0381.
GeneID	1044091.
KEGG	ppu:PP_0381.
PATRIC	19938803. VBIPsePut30601_0410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1025.
HOGENOM	HOG000267295.
OMA	QVNASTR.
ProtClustDB	CLSK866926.
Enzyme and pathway databases
BioCyc	PPUT160488:GIXO-382-MONOMER.
UniPathway	UPA00539.
Family and domain databases
Gene3D	3.30.830.10. 2 hits.
InterPro	IPR011249. Metalloenz_LuxS/M16. IPR011237. Pept_M16_dom. IPR011765. Pept_M16_N. IPR001431. Pept_M16_Zn_BS. IPR011844. Pept_M16A_PQQ_synth_PqqF. [Graphical view]
Pfam	PF00675. Peptidase_M16. 1 hit. [Graphical view]
SUPFAM	SSF63411. Metalloenz_metal-bd. 2 hits.
TIGRFAMs	TIGR02110. PQQ_syn_pqqF. 1 hit.
PROSITE	PS00143. INSULINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PQQF_PSEPK

Accession

Primary (citable) accession number: Q88QV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program