Reviewed, UniProtKB/Swiss-Prot Q88QT8 (APAH_PSEPK)

Names and origin

Protein names

Recommended name:
    Bis(5'-nucleosyl)-tetraphosphatase, symmetrical
    EC=3.6.1.41
Alternative name(s):
    Diadenosine tetraphosphatase
    Ap4A hydrolase
    Diadenosine 5',5'''-P1,P4-tetraphosphate pyrophosphohydrolase

Gene names

Name:	apaH
Ordered Locus Names:	PP_0399

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Hydrolyzes diadenosine 5',5'''-P1,P4-tetraphosphate to yield ADP By similarity.
Catalytic activity	P1,P(4)-bis(5'-adenosyl) tetraphosphate + H2O = 2 ADP. HAMAP MF_00199
Sequence similarities	Belongs to the Ap4A hydrolase family.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Bis(5'-nucleosyl)-tetraphosphatase, symmetrical HAMAP MF_00199		PRO_0000198005

Sequences

	Sequence		Length	Mass (Da)
	Q88QT8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EA26F763D93BB19D	FASTA	288	32,603
	10 20 30 40 50 60 MATYAVGDLQ GCLQPLKCLL DRVSFNPAVD RLWLVGDLVN RGPESLETLR FLYSIRHSLV 70 80 90 100 110 120 CVLGNHDLHL LAAWHNVERL KKSDTLREII EAPDADQLFD WLRQQKLLHY DEPRGIALVH 130 140 150 160 170 180 AGIPPQWTLG KALELAAEVE EVLRDDTRLQ LYLDGMYGNE PNKWSKNLAG VERLRVITNY 190 200 210 220 230 240 FTRMRFCTAD GKLDLKSKEG LGSAPKGYKA WYAHKDRRSR HVKIIFGHWA ALQGEVTEPD 250 260 270 280 VIALDTGCVW GGAMTLYNVD SGEYHRCDCA DDGTLRQPAQ PTTLNDHT

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66030.1.
RefSeq	NP_742566.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044113.
GenomeReviews	Gene locus PP_0399 in contig AE015451_GR.
KEGG	ppu:PP_0399.
NMPDR	fig|160488.1.peg.396.
TIGR	PP_0399.
Phylogenomic databases
HOGENOM	Q88QT8.
OMA	Q88QT8. LVFGHWA.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0399-MON.
Family and domain databases
HAMAP	MF_00199. [Tree]
InterPro	IPR004617. ApaH. IPR004843. M-pesterase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PIRSF	PIRSF000903. B5n-ttraPtase_sm. 1 hit.
TIGRFAMs	TIGR00668. apaH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

APAH_PSEPK

Accession

Primary (citable) accession number: Q88QT8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)