Reviewed, UniProtKB/Swiss-Prot Q88QS2 (GPH_PSEPK)

Names and origin

Protein names

Recommended name:
    Phosphoglycolate phosphatase
      Short name=PGPase
      Short name=PGP
    EC=3.1.3.18

Gene names

Ordered Locus Names:

PP_0416

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the dephosphorylation of 2-phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stress By similarity.
Catalytic activity	2-phosphoglycolate + H2O = glycolate + phosphate. HAMAP MF_00495
Pathway	Organic acid metabolism; glycolic acid biosynthesis; glycolic acid from 2-phosphoglycolic acid: step 1/1. HAMAP MF_00495
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. CbbY/cbbZ/gph/yieH family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	phosphoglycolate phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Phosphoglycolate phosphatase HAMAP MF_00495		PRO_0000238168
Sites
	Active site	19	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88QS2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1D91E2F4885ADED9	FASTA	272	29,604
	10 20 30 40 50 60 MSGFEQLFPG TLPRLVMFDL DGTLIDSVPD LAAAVDRMLL ELGRPPAGLE AVRHWVGNGA 70 80 90 100 110 120 QVLVRRALAG GIDHADVDDA LAEQALALFM DAYAESHELT VVYPGVRDTL RWLRKQGVEM 130 140 150 160 170 180 ALITNKPERF VGPLLDQMKI GNFFRWIIGG DTLPQKKPDP AALLFVMQMA GVTPQQSLFV 190 200 210 220 230 240 GDSRSDVQAA KAAGVQCVGL TYGYNHGRPI DAESPSLVID DLRALLPGCA DPATGITLAD 250 260 270 LQASQDREST VAVTGKFWMK VIKALARWRW RA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66046.1.
RefSeq	NP_742582.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044138.
GenomeReviews	Gene locus PP_0416 in contig AE015451_GR.
KEGG	ppu:PP_0416.
NMPDR	fig|160488.1.peg.412.
TIGR	PP_0416.
Phylogenomic databases
HOGENOM	Q88QS2.
OMA	Q88QS2. AVCEQFS.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0416-MON.
Family and domain databases
HAMAP	MF_00495. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR006439. HAD-SF_hydro_IA_v1. IPR006402. HAD-SF_hydro_IA_v3. IPR005833. Haloacid_DH/epoxide_hydro. IPR006346. PGP_bact. [Graphical view]
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00413. HADHALOGNASE.
TIGRFAMs	TIGR01549. HAD-SF-IA-v1. 1 hit. TIGR01509. HAD-SF-IA-v3. 1 hit. TIGR01449. PGP_bact. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GPH_PSEPK

Accession

Primary (citable) accession number: Q88QS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)