Reviewed, UniProtKB/Swiss-Prot Q88QC7 (SPEA_PSEPK)

Names and origin

Protein names

Recommended name:
    Biosynthetic arginine decarboxylase
      Short name=ADC
    EC=4.1.1.19

Gene names

Name:	speA
Ordered Locus Names:	PP_0567

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	637 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the biosynthesis of agmatine from arginine By similarity.
Catalytic activity	L-arginine = agmatine + CO2. HAMAP MF_01417
Cofactor	Magnesium By similarity. Pyridoxal phosphate By similarity.
Sequence similarities	Belongs to the Orn/Lys/Arg decarboxylase class-II family. SpeA subfamily.

Ontologies

Keywords
Biological process	Polyamine biosynthesis Spermidine biosynthesis
Ligand	Magnesium Metal-binding Pyridoxal phosphate
Molecular function	Decarboxylase Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process Inferred from electronic annotation. Source: InterPro spermidine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	arginine decarboxylase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 637	637	Biosynthetic arginine decarboxylase HAMAP MF_01417		PRO_0000149972
Regions
	Region	290 – 300	11	Substrate-binding Potential
Amino acid modifications
	Modified residue	110	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88QC7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E59F5EA60F345738	FASTA	637	71,043
	10 20 30 40 50 60 MSVRRTRKDD GSQWTVADSR SVYGIRHWGA GYFAINEAGR VEVRPNGPQS APIDLFEQVD 70 80 90 100 110 120 ELRQSGLSLP LLVRFPDILQ DRVRQLTGAF DANIARLEYQ SQYTALYPIK VNQQEAVVEN 130 140 150 160 170 180 IIATQNVSIG LEAGSKPELL AVLALAPKGG TIVCNGYKDR EFIRLALMGQ KLGHNVFIVI 190 200 210 220 230 240 EKESEVALVI EEAAELKVKP QVGLRVRLSS LASSKWADTG GEKSKFGLSA AQLLSVVQRF 250 260 270 280 290 300 RDAGLDQGIR LLHFHMGSQI ANLADYQHGF KEAIRYYGEL RALGLPVDHI DVGGGLGVDY 310 320 330 340 350 360 DGTHSRNASS INYDMDDYAG VVVGMLKEFC DAQGLPHPHI FSESGRSLTA HHAMLVIQVT 370 380 390 400 410 420 DVEKHNDDVP TIENKEALPE TVQWLVDLLG PTDIEMVTET YWRATHYMGD VAAQYADGKL 430 440 450 460 470 480 SLGEKALAEQ CYFAVCRRLH NSLKARQRSH RQVLDELNDK LADKYICNFS VFQSLPDTWA 490 500 510 520 530 540 IGQVLPIIPL HRLDEEPMRR AVLQDLTCDS DGKINQYVDE QSIETSMPVH AVKEGEDYLL 550 560 570 580 590 600 GVFLVGAYQE ILGDMHNLFG DTDSVNIYQN ADGSVYHAGI ETHDTIEDML RYVHLSPEEL 610 620 630 MTHYRDKVAS AKITARERTQ YLDALRLGLT RSSYLSS

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66194.1.
RefSeq	NP_742730.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044357.
GenomeReviews	Gene locus PP_0567 in contig AE015451_GR.
KEGG	ppu:PP_0567.
NMPDR	fig|160488.1.peg.560.
TIGR	PP_0567.
Phylogenomic databases
HOGENOM	Q88QC7.
OMA	Q88QC7. LICNGYK.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0567-MON.
Family and domain databases
HAMAP	MF_01417. [Tree]
InterPro	IPR002985. Arg_decrbxlase. IPR000183. De-COase2. [Graphical view]
PANTHER	PTHR11482:SF3. Arg_decrbxlase. 1 hit.
Pfam	PF02784. Orn_Arg_deC_N. 1 hit. PF00278. Orn_DAP_Arg_deC. 1 hit. [Graphical view]
PIRSF	PIRSF001336. Arg_decrbxlase. 1 hit.
PRINTS	PR01180. ARGDCRBXLASE. PR01179. ODADCRBXLASE.
TIGRFAMs	TIGR01273. speA. 1 hit.
PROSITE	PS00878. ODR_DC_2_1. False negative. PS00879. ODR_DC_2_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPEA_PSEPK

Accession

Primary (citable) accession number: Q88QC7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 29, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)