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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi206 – 213ATP 1By similarity8
Nucleotide bindingi605 – 612ATP 2By similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone
Biological processStress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-682-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:PP_0625
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001911621 – 854Chaperone protein ClpBAdd BLAST854

Proteomic databases

PRIDEiQ88Q71

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi160488.PP_0625

Structurei

3D structure databases

ProteinModelPortaliQ88Q71
SMRiQ88Q71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 143N-terminalBy similarityAdd BLAST143
Regioni159 – 340NBD1By similarityAdd BLAST182
Regioni341 – 545LinkerBy similarityAdd BLAST205
Regioni555 – 763NBD2By similarityAdd BLAST209
Regioni764 – 854C-terminalBy similarityAdd BLAST91

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili391 – 524By similarityAdd BLAST134

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z Bacteria
COG0542 LUCA
HOGENOMiHOG000218209
KOiK03695
OMAiHHKVRIK

Family and domain databases

Gene3Di1.10.1780.10, 1 hit
InterProiView protein in InterPro
IPR003593 AAA+_ATPase
IPR003959 ATPase_AAA_core
IPR017730 Chaperonin_ClpB
IPR019489 Clp_ATPase_C
IPR004176 Clp_N
IPR036628 Clp_N_dom_sf
IPR001270 ClpA/B
IPR018368 ClpA/B_CS1
IPR028299 ClpA/B_CS2
IPR027417 P-loop_NTPase
IPR002078 Sigma_54_int
PfamiView protein in Pfam
PF00004 AAA, 1 hit
PF07724 AAA_2, 1 hit
PF02861 Clp_N, 2 hits
PF10431 ClpB_D2-small, 1 hit
PRINTSiPR00300 CLPPROTEASEA
SMARTiView protein in SMART
SM00382 AAA, 2 hits
SM01086 ClpB_D2-small, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
SSF81923 SSF81923, 1 hit
TIGRFAMsiTIGR03346 chaperone_ClpB, 1 hit
PROSITEiView protein in PROSITE
PS00870 CLPAB_1, 1 hit
PS00871 CLPAB_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q88Q71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIDRLTSKL QLAISDAQSL AVGMDHPAIE PVHLLQALLE QQGGSIKPLL
60 70 80 90 100
MQVGFDINGL RQGLVKELDQ LPKIQNPTGD VNMSQDLARL LNQADRLAQQ
110 120 130 140 150
KGDQFISSEL VLLAAMDENS KLGKLLLSQG VSKKALENAI NNLRGGAAVN
160 170 180 190 200
DANAEESRQA LDKYTVDLTK RAEEGKLDPV IGRDDEIRRT VQVLQRRTKN
210 220 230 240 250
NPVLIGEPGV GKTAIAEGLA QRIINGEVPD GLKGKRLLAL DMGALIAGAK
260 270 280 290 300
YRGEFEERLK SLLNELSKQE GQIILFIDEL HTMVGAGKGE GAMDAGNMLK
310 320 330 340 350
PALARGELHC VGATTLNEYR QFIEKDAALE RRFQKVLVEE PSEEDTIAIL
360 370 380 390 400
RGLKERYEVH HKVAITDGAI IAAAKLSHRY ITDRQLPDKA IDLIDEAASR
410 420 430 440 450
IRMEIDSKPE VLDRLDRRLI QLKVESQALK KEEDEAAKKR LEKLTEEIER
460 470 480 490 500
LEREYSDLEE IWASEKAEVQ GSAQIQQKIE QSRQELEAAR RKGDLNRMAE
510 520 530 540 550
LQYGVIPDLE RSLQMVDQHG KTDNQLLRNK VTEEEIAEVV SKWTGIPVAK
560 570 580 590 600
MLEGEREKLL KMEELLHQRV IGQSEAVTAV ANAVRRSRAG LSDPNRPSGS
610 620 630 640 650
FLFLGPTGVG KTELCKALAE FLFDTEEAMV RIDMSEFMEK HSVARLIGAP
660 670 680 690 700
PGYVGYEEGG YLTEAVRRKP YSVVLLDEVE KAHPDVFNVL LQVLEDGRLT
710 720 730 740 750
DSHGRTVDFR NTVIVMTSNL GSAQIQELVG DREAQRAAVM DAVGAHFRPE
760 770 780 790 800
FINRIDEVVV FEPLGREQIA GITEIQLGRL RSRLLERELS LSLSPEALDK
810 820 830 840 850
LIAVGYDPVY GARPLKRAIQ RWIENPLAQL ILAGKFLPGT AITAKVEGDE

IVFG
Length:854
Mass (Da):94,862
Last modified:June 1, 2003 - v1
Checksum:i7750EEFA06D9F534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66250.1
RefSeqiNP_742786.1, NC_002947.4
WP_010951887.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66250; AAN66250; PP_0625
GeneIDi1044444
KEGGippu:PP_0625
PATRICifig|160488.4.peg.666

Similar proteinsi

Entry informationi

Entry nameiCLPB_PSEPK
AccessioniPrimary (citable) accession number: Q88Q71
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 94 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health