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Protein

Chaperone protein ClpB

Gene

clpB

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase activity; ATP hydrolysis unfolds the denatured protein aggregates, which probably helps expose new hydrophobic binding sites on the surface of ClpB-bound aggregates, contributing to the solubilization and refolding of denatured protein aggregates by DnaK (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi206 – 2138ATP 1By similarity
Nucleotide bindingi605 – 6128ATP 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-648-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein ClpB
Gene namesi
Name:clpB
Ordered Locus Names:PP_0625
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 854854Chaperone protein ClpBPRO_0000191162Add
BLAST

Proteomic databases

PRIDEiQ88Q71.

Interactioni

Subunit structurei

Homohexamer. The oligomerization is ATP-dependent (By similarity).By similarity

Protein-protein interaction databases

STRINGi160488.PP_0625.

Structurei

3D structure databases

ProteinModelPortaliQ88Q71.
SMRiQ88Q71. Positions 159-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 143143N-terminalBy similarityAdd
BLAST
Regioni159 – 340182NBD1By similarityAdd
BLAST
Regioni341 – 545205LinkerBy similarityAdd
BLAST
Regioni555 – 763209NBD2By similarityAdd
BLAST
Regioni764 – 85491C-terminalBy similarityAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili391 – 524134By similarityAdd
BLAST

Domaini

The N-terminal domain probably functions as a substrate-discriminating domain, recruiting aggregated proteins to the ClpB hexamer and/or stabilizing bound proteins. The NBD2 domain is responsible for oligomerization, whereas the NBD1 domain stabilizes the hexamer probably in an ATP-dependent manner. The movement of the coiled-coil domain is essential for ClpB ability to rescue proteins from an aggregated state, probably by pulling apart large aggregated proteins, which are bound between the coiled-coils motifs of adjacent ClpB subunits in the functional hexamer (By similarity).By similarity

Sequence similaritiesi

Belongs to the ClpA/ClpB family.Curated

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218209.
KOiK03695.
OMAiPVERILM.
OrthoDBiEOG65F8SM.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88Q71-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIDRLTSKL QLAISDAQSL AVGMDHPAIE PVHLLQALLE QQGGSIKPLL
60 70 80 90 100
MQVGFDINGL RQGLVKELDQ LPKIQNPTGD VNMSQDLARL LNQADRLAQQ
110 120 130 140 150
KGDQFISSEL VLLAAMDENS KLGKLLLSQG VSKKALENAI NNLRGGAAVN
160 170 180 190 200
DANAEESRQA LDKYTVDLTK RAEEGKLDPV IGRDDEIRRT VQVLQRRTKN
210 220 230 240 250
NPVLIGEPGV GKTAIAEGLA QRIINGEVPD GLKGKRLLAL DMGALIAGAK
260 270 280 290 300
YRGEFEERLK SLLNELSKQE GQIILFIDEL HTMVGAGKGE GAMDAGNMLK
310 320 330 340 350
PALARGELHC VGATTLNEYR QFIEKDAALE RRFQKVLVEE PSEEDTIAIL
360 370 380 390 400
RGLKERYEVH HKVAITDGAI IAAAKLSHRY ITDRQLPDKA IDLIDEAASR
410 420 430 440 450
IRMEIDSKPE VLDRLDRRLI QLKVESQALK KEEDEAAKKR LEKLTEEIER
460 470 480 490 500
LEREYSDLEE IWASEKAEVQ GSAQIQQKIE QSRQELEAAR RKGDLNRMAE
510 520 530 540 550
LQYGVIPDLE RSLQMVDQHG KTDNQLLRNK VTEEEIAEVV SKWTGIPVAK
560 570 580 590 600
MLEGEREKLL KMEELLHQRV IGQSEAVTAV ANAVRRSRAG LSDPNRPSGS
610 620 630 640 650
FLFLGPTGVG KTELCKALAE FLFDTEEAMV RIDMSEFMEK HSVARLIGAP
660 670 680 690 700
PGYVGYEEGG YLTEAVRRKP YSVVLLDEVE KAHPDVFNVL LQVLEDGRLT
710 720 730 740 750
DSHGRTVDFR NTVIVMTSNL GSAQIQELVG DREAQRAAVM DAVGAHFRPE
760 770 780 790 800
FINRIDEVVV FEPLGREQIA GITEIQLGRL RSRLLERELS LSLSPEALDK
810 820 830 840 850
LIAVGYDPVY GARPLKRAIQ RWIENPLAQL ILAGKFLPGT AITAKVEGDE

IVFG
Length:854
Mass (Da):94,862
Last modified:June 1, 2003 - v1
Checksum:i7750EEFA06D9F534
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66250.1.
RefSeqiNP_742786.1. NC_002947.4.
WP_010951887.1. NC_002947.4.

Genome annotation databases

EnsemblBacteriaiAAN66250; AAN66250; PP_0625.
GeneIDi1044444.
KEGGippu:PP_0625.
PATRICi19939341. VBIPsePut30601_0666.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66250.1.
RefSeqiNP_742786.1. NC_002947.4.
WP_010951887.1. NC_002947.4.

3D structure databases

ProteinModelPortaliQ88Q71.
SMRiQ88Q71. Positions 159-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_0625.

Proteomic databases

PRIDEiQ88Q71.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN66250; AAN66250; PP_0625.
GeneIDi1044444.
KEGGippu:PP_0625.
PATRICi19939341. VBIPsePut30601_0666.

Phylogenomic databases

eggNOGiENOG4105C2Z. Bacteria.
COG0542. LUCA.
HOGENOMiHOG000218209.
KOiK03695.
OMAiPVERILM.
OrthoDBiEOG65F8SM.

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-648-MONOMER.

Family and domain databases

Gene3Di1.10.1780.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR003959. ATPase_AAA_core.
IPR017730. Chaperonin_ClpB.
IPR019489. Clp_ATPase_C.
IPR004176. Clp_N.
IPR001270. ClpA/B.
IPR018368. ClpA/B_CS1.
IPR028299. ClpA/B_CS2.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
[Graphical view]
PfamiPF00004. AAA. 1 hit.
PF07724. AAA_2. 1 hit.
PF02861. Clp_N. 2 hits.
PF10431. ClpB_D2-small. 1 hit.
[Graphical view]
PRINTSiPR00300. CLPPROTEASEA.
SMARTiSM00382. AAA. 2 hits.
SM01086. ClpB_D2-small. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF81923. SSF81923. 1 hit.
TIGRFAMsiTIGR03346. chaperone_ClpB. 1 hit.
PROSITEiPS00870. CLPAB_1. 1 hit.
PS00871. CLPAB_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiCLPB_PSEPK
AccessioniPrimary (citable) accession number: Q88Q71
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: June 1, 2003
Last modified: May 11, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.