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Protein

Probable malate dehydrogenase

Gene

mdh

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511SubstrateBy similarity
Binding sitei57 – 571SubstrateBy similarity
Binding sitei64 – 641NADBy similarity
Binding sitei89 – 891SubstrateBy similarity
Binding sitei120 – 1201SubstrateBy similarity
Active sitei144 – 1441Proton acceptorBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi87 – 893NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-683-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable malate dehydrogenase (EC:1.1.1.37)
Gene namesi
Name:mdh
Ordered Locus Names:PP_0654
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Probable malate dehydrogenasePRO_0000113460Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_0654.

Structurei

3D structure databases

ProteinModelPortaliQ88Q44.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. MDH type 3 family.Curated

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiFRYIMAE.
OrthoDBiEOG6091FG.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88Q44-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVQGELAQG KALDVWQAAV DSGSDTHVHG GAKAEMLEGS ELVVITAGVP
60 70 80 90 100
RKPGQSRQDV LSTNLPILDS IMADIKHHAP TATVLVVSNP VDVLTYRAWS
110 120 130 140 150
VSGQGRDKVF GQAGVLDTAR MKCFIAEQTG FSARDITALV LGGHGDSMVP
160 170 180 190 200
LMRYCQIGSV PLSHFLSSEQ IEQIVERTRK GGGEILGLKK TGSACDAPGV
210 220 230 240 250
AIAQMVDAIA NGRNRILPAV AILEGEYGRT DIAMGVPCVL AEKGLARIIE
260 270
LPLDAQEQAM FDHSADQVAR DIAEMKAL
Length:278
Mass (Da):29,434
Last modified:June 1, 2003 - v1
Checksum:i9F48BC27CC4D54C5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66279.1.
RefSeqiNP_742815.1. NC_002947.3.
WP_010951912.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN66279; AAN66279; PP_0654.
GeneIDi1044477.
KEGGippu:PP_0654.
PATRICi19939419. VBIPsePut30601_0699.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66279.1.
RefSeqiNP_742815.1. NC_002947.3.
WP_010951912.1. NC_002947.3.

3D structure databases

ProteinModelPortaliQ88Q44.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_0654.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN66279; AAN66279; PP_0654.
GeneIDi1044477.
KEGGippu:PP_0654.
PATRICi19939419. VBIPsePut30601_0699.

Phylogenomic databases

eggNOGiCOG0039.
HOGENOMiHOG000213794.
KOiK00024.
OMAiFRYIMAE.
OrthoDBiEOG6091FG.

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-683-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiMDH_PSEPK
AccessioniPrimary (citable) accession number: Q88Q44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 2003
Last modified: July 22, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.