Reviewed, UniProtKB/Swiss-Prot Q88Q27 (GLYA2_PSEPK)

Names and origin

Protein names

Recommended name:
    Serine hydroxymethyltransferase 2
      Short name=Serine methylase 2
      Short name=SHMT 2
    EC=2.1.2.1

Gene names

Name:	glyA2
Synonyms:	glyA-2
Ordered Locus Names:	PP_0671

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	417 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Interconversion of serine and glycine. HAMAP MF_00051
Catalytic activity	5,10-methylenetetrahydrofolate + glycine + H2O = tetrahydrofolate + L-serine. HAMAP MF_00051
Cofactor	Pyridoxal phosphate By similarity.
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP MF_00051
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the SHMT family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine metabolic process Inferred from electronic annotation. Source: InterPro glycine metabolic process Inferred from electronic annotation. Source: InterPro one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glycine hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 417	417	Serine hydroxymethyltransferase 2 HAMAP MF_00051		PRO_0000113641
Amino acid modifications
	Modified residue	229	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88Q27-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: 2F7ACCD557B2938E	FASTA	417	44,897
	10 20 30 40 50 60 MFSRDLTIAK YDAELFEAMQ QEALRQEEHI ELIASENYTS PAVMEAQGSV LTNKYAEGYP 70 80 90 100 110 120 GKRYYGGCEY VDVVEQLAID RAKELFGADY ANVQPHAGSQ ANAAVYLALL SAGDTILGMS 130 140 150 160 170 180 LAHGGHLTHG ASVSSSGKLY NAIQYGIDGN GLIDYDEVER LAVEHKPKMI VAGFSAYSQV 190 200 210 220 230 240 LDFARFRAIA DKVGAYLFVD MAHVAGLVAA GVYPNPVPFA DVVTTTTHKT LRGPRGGLIL 250 260 270 280 290 300 ARANADIEKK LNSAVFPGAQ GGPLEHVIAA KAICFKEALQ PEFKAYQQQV VKNAQAMASV 310 320 330 340 350 360 FIERGFDVVS GGTQNHLFLL SLIKQEISGK DADAALGKAF ITVNKNSVPN DPRSPFVTSG 370 380 390 400 410 LRFGTPAVTT RGFKEAECKE LAGWICDILA DLNNEAVIDA VREKVKAICK KLPVYGN

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66296.1.
RefSeq	NP_742832.1.
3D structure databases
HSSP	HSSP built from PDB template 1DFO based on UniProtKB P00477.
SMR	Q88Q27. Positions 1-416.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88Q27.
Genome annotation databases
GeneID	1044495.
GenomeReviews	Gene locus PP_0671 in contig AE015451_GR.
KEGG	ppu:PP_0671.
NMPDR	fig|160488.1.peg.662.
TIGR	PP_0671.
Phylogenomic databases
HOGENOM	Q88Q27.
OMA	NNAKAMV.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0671-MON.
Family and domain databases
HAMAP	MF_00051. [Tree]
InterPro	IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR001085. Ser_HO-MeTrfase. IPR019798. Ser_HO-MeTrfase_PLP_BS. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11680. Gly_HO-Metrfase. 1 hit.
Pfam	PF00464. SHMT. 1 hit. [Graphical view]
PIRSF	PIRSF000412. SHMT. 1 hit.
PROSITE	PS00096. SHMT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLYA2_PSEPK

Accession

Primary (citable) accession number: Q88Q27

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)