Reviewed, UniProtKB/Swiss-Prot Q88P77 (RLMF_PSEPK)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase F
    EC=2.1.1.48
Alternative name(s):
    23S rRNA mA1618 methyltransferase
    rRNA adenine N-6-methyltransferase

Gene names

Name:	rlmF
Ordered Locus Names:	PP_0976

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the adenine in position 1618 of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(6)-methyladenine. HAMAP MF_01848
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. METT10D/rlmF family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (adenine-N6-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Ribosomal RNA large subunit methyltransferase F HAMAP MF_01848		PRO_0000349934

Sequences

	Sequence		Length	Mass (Da)
	Q88P77-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: ABB77F17CA7B2D3C	FASTA	317	35,187
	10 20 30 40 50 60 MTPNKPTLHP RNRHQGRYDF PSLIKAHPDL ARFTITNPHG KPSIDFANPE AVRVFNRALL 70 80 90 100 110 120 KAQYGIQHWD IPADYLCPPI PGRADYIHVA ADLLADDNAG DVPRGAQVRA LDIGVGANCI 130 140 150 160 170 180 YPLLGYSDYR WRFLGSDIDP VALASAKAIV QANGLGKAIT LRQQANRAHI LSGLLQEGER 190 200 210 220 230 240 FDLTLCNPPF HASRDEATRG SQRKWKNLGK QDPKRKLPVL NFGGQNNELW CEGGEIRFVT 250 260 270 280 290 300 QLIGESVQYA ERVLWFTSLV SKASNLPGIE AALKKSGAKA VRIIEMGQGQ KQSRMVAWSF 310 LDDAARQAWH AQRKSQA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66601.1.
RefSeq	NP_743137.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044897.
GenomeReviews	Gene locus PP_0976 in contig AE015451_GR.
KEGG	ppu:PP_0976.
NMPDR	fig|160488.1.peg.967.
TIGR	PP_0976.
Phylogenomic databases
HOGENOM	Q88P77.
OMA	Q88P77. LNFGGQN.
Family and domain databases
HAMAP	MF_01848. [Tree]
InterPro	IPR016909. S-AdoMet-dep_MeTrfase_YbiN_prd. IPR010286. SAM-Mtase_prd. [Graphical view]
PANTHER	PTHR13393. DUF890. 1 hit.
Pfam	PF05971. Methyltransf_10. 1 hit. [Graphical view]
PIRSF	PIRSF029038. Mtase_YbiN_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMF_PSEPK

Accession

Primary (citable) accession number: Q88P77

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 26 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)