Reviewed, UniProtKB/Swiss-Prot Q88P65 (GCSP1_PSEPK)

Names and origin

Protein names

Recommended name:
    Glycine dehydrogenase [decarboxylating] 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase 1
    Glycine cleavage system P-protein 1

Gene names

Name:	gcvP1
Synonyms:	gcvP-1
Ordered Locus Names:	PP_0988

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	951 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00711
Cofactor	Pyridoxal phosphate By similarity.
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the gcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 951	951	Glycine dehydrogenase [decarboxylating] 1 HAMAP MF_00711		PRO_0000166927
Amino acid modifications
	Modified residue	703	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88P65-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EDDBDB715CDE148E	FASTA	951	101,992
	10 20 30 40 50 60 MTINLGTANE FIARHIGPRA ADEQAMLTAL GFDSLDAMTA AVIPDSIKGT SVLGSHDGQS 70 80 90 100 110 120 EADALAALKA IAGKNQLFKS YIGQGYYNTH TPAPILRNLL ENPAWYTAYT PYQPEISQGR 130 140 150 160 170 180 LEALLNFQTL ISDLTGLPIA NASLLDEATA AAEAMTFCKR LSKNKSSHAF FASVHCHPQT 190 200 210 220 230 240 LDVLRTRAEP LGIEVVVGDE RELGDVSAFF GALLQYPASN GEVFDYREVV QRFHAANALV 250 260 270 280 290 300 AVAADLLALT LLTPPGEFDA DVAIGSAQRF GVPLGFGGPH AAYFATRDAF KRDMPGRLVG 310 320 330 340 350 360 VSIDRFGKTA LRLAMQTREQ HIRREKATSN ICTAQVLLAN IASMFAVYHG PAGLKRIAER 370 380 390 400 410 420 THALTAILAA GLKALGVQVV GASAFDTLTL ATGTATASLH DKARAQGINL RQIDAAHVGL 430 440 450 460 470 480 SLDETSTQAD VESLWQLLGG EQAQPDFTAL AASTGSLLPA ALLRQSAILE HPVFNRYHSE 490 500 510 520 530 540 TELMRYLRRL ADKDLALDRS MIPLGSCTMK LNAASEMIPV TWAEFGNLHP FAPAEQSQGY 550 560 570 580 590 600 LQMTTELEAM LCAATGYDAV SLQPNAGSQG EYAGLLAIRA YHRSRGEGHR DICLIPSSAH 610 620 630 640 650 660 GTNPATAHMA GMRVVVTACD ARGNVDVEDL RAKAIEHRER LAAIMITYPS THGVFEEAIG 670 680 690 700 710 720 EICAIIHDNG GQVYIDGANM NAMVGLCAPG KFGGDVSHLN LHKTFCIPHG GGGPGVGPIG 730 740 750 760 770 780 VKSHLAPFLP GHAQLENTQG AVCAAPFGSA SILPITWMYI RMMGGAGLKR ASQMAILNAN 790 800 810 820 830 840 YIARRLEEHY PVLYTGGNGL VAHECILDLR PLKDTSGISV DDVAKRLIDF GFHAPTMSFP 850 860 870 880 890 900 VAGTLMIEPT ESESKEELDR FCNAMIQIRE EIRAVEDGSL DKDDNPLKNA PHTAAELVGE 910 920 930 940 950 WTHGYSREQA VYPLASLVEG KYWPPVGRVD NVFGDRNLVC ACPSIESYQD A

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66613.1.
RefSeq	NP_743149.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1044914.
GenomeReviews	Gene locus PP_0988 in contig AE015451_GR.
KEGG	ppu:PP_0988.
NMPDR	fig|160488.1.peg.979.
TIGR	PP_0988.
Phylogenomic databases
HOGENOM	Q88P65.
OMA	Q88P65. NRAESEM.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_0988-MON.
Family and domain databases
HAMAP	MF_00711. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP1_PSEPK

Accession

Primary (citable) accession number: Q88P65

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)