Reviewed, UniProtKB/Swiss-Prot Q88P53 (OTCC_PSEPK)

Names and origin

Protein names

Recommended name:
    Ornithine carbamoyltransferase, catabolic
      Short name=OTCase
    EC=2.1.3.3

Gene names

Name:	arcB
Ordered Locus Names:	PP_1000

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine: step 2/2. HAMAP MF_01109
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Arginine metabolism
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 336	335	Ornithine carbamoyltransferase, catabolic HAMAP MF_01109		PRO_0000112989
Regions
	Region	57 – 61	5	Carbamoyl phosphate binding By similarity
	Region	273 – 276	4	Ornithine binding By similarity
Sites
	Binding site	108	1	Carbamoyl phosphate By similarity
	Binding site	135	1	Carbamoyl phosphate By similarity
	Site	32	1	Important for structural integrity By similarity
	Site	148	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88P53-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: AADD3031207D0935	FASTA	336	37,911
	10 20 30 40 50 60 MAFNIHNRNL LSLEHHTTRE LRYLLDLSRD LKRAKYTGTE QQHLKGNNIA LIFEKTSTRT 70 80 90 100 110 120 RCAFEVAAYD QGANVTYIDP NSSQIGHKES MKDTARVLGR MYDAIEYRGF KQEIVEELAK 130 140 150 160 170 180 FAGVPVFNGL TDEYHPTQMI ADVLTMREHS DKPLHDISYA YLGDARNNMG NSLLLIGAKL 190 200 210 220 230 240 GMDVRIAAPK ALWPHDDLVE RCKQYAEESG ARITLTEDPK AAVKGVDFVH TDVWVSMGEP 250 260 270 280 290 300 IEAWGERIKQ LKPYQVNAEL MKSTGNPRTK FMHCLPAFHN SETKVGKQIA EQYPDLANGI 310 320 330 EVTDDVFESP ACIAFEQAEN RMHTIKAILV STLADL

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66625.1.
RefSeq	NP_743161.1.
3D structure databases
HSSP	HSSP built from PDB template 1DXH based on UniProtKB P08308.
SMR	Q88P53. Positions 2-336.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88P53.
Genome annotation databases
GeneID	1044931.
GenomeReviews	Gene locus PP_1000 in contig AE015451_GR.
KEGG	ppu:PP_1000.
NMPDR	fig|160488.1.peg.991.
TIGR	PP_1000.
Phylogenomic databases
HOGENOM	Q88P53.
OMA	NPNVKFM.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_1000-MON.
Family and domain databases
HAMAP	MF_01109. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P_bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn_bd. IPR002292. Orn_carbamltrans. [Graphical view]
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
TIGRFAMs	TIGR00658. orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTCC_PSEPK

Accession

Primary (citable) accession number: Q88P53

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 51 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)