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Protein

Membrane-bound lytic murein transglycosylase F

Gene

mltF

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the peptidoglycan (PG) sacculus. Their lytic action creates space within the PG sacculus to allow for its expansion as well as for the insertion of various structures such as secretion systems and flagella.UniRule annotation

Catalytic activityi

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei314UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1109-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane-bound lytic murein transglycosylase FUniRule annotation (EC:4.2.2.n1UniRule annotation)
Alternative name(s):
Murein lyase FUniRule annotation
Gene namesi
Name:mltFUniRule annotation
Ordered Locus Names:PP_1036
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cell outer membrane ; Peripheral membrane protein
  • Note: Attached to the inner leaflet of the outer membrane.UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29UniRule annotationAdd BLAST29
ChainiPRO_000035396430 – 485Membrane-bound lytic murein transglycosylase FAdd BLAST456

Proteomic databases

PRIDEiQ88P17

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_1036

Structurei

3D structure databases

ProteinModelPortaliQ88P17
SMRiQ88P17
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni30 – 267Non-LT domainUniRule annotationAdd BLAST238
Regioni268 – 485LT domainUniRule annotationAdd BLAST218

Domaini

The N-terminal domain does not have lytic activity and probably modulates enzymatic activity. The C-terminal domain is the catalytic active domain.UniRule annotation

Sequence similaritiesi

In the N-terminal section; belongs to the bacterial solute-binding protein 3 family.UniRule annotation
In the C-terminal section; belongs to the transglycosylase Slt family.UniRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105CHQ Bacteria
COG4623 LUCA
HOGENOMiHOG000218316
KOiK18691
OMAiYYDILTW

Family and domain databases

HAMAPiMF_02016 MltF, 1 hit
InterProiView protein in InterPro
IPR023346 Lysozyme-like_dom_sf
IPR023703 MltF
IPR001638 Solute-binding_3/MltF_N
IPR000189 Transglyc_AS
IPR008258 Transglycosylase_SLT_dom_1
PfamiView protein in Pfam
PF00497 SBP_bac_3, 1 hit
PF01464 SLT, 1 hit
SMARTiView protein in SMART
SM00062 PBPb, 1 hit
SUPFAMiSSF53955 SSF53955, 2 hits
PROSITEiView protein in PROSITE
PS00922 TRANSGLYCOSYLASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q88P17-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFAHTALRQR CAKWLLATGL FLLLGACVEK PSTLERVKED GVLRVITRNS
60 70 80 90 100
PATYFQDRNG ETGFEYELVQ HFADDLGVKL QIETADNLDE LYDALGKPSG
110 120 130 140 150
PVLAAAGLVS SERRKTQVRY SHPYLEVTPQ VIYRNGRPRP TGAKGLVGKK
160 170 180 190 200
IMVLKGSSHA DQLAELKKQY PALQYEESDA VEVVDLLRMV DEGQIDLTLV
210 220 230 240 250
DSNELAMNQV YFPNVRVAFD LGETRDQRWA VAAGEDNSLL NEINEYLDKA
260 270 280 290 300
QKNGTLQRLK DRYYGHVDVL GYVGAYTFAQ HLQQRLPKYE KHFKSYAKVE
310 320 330 340 350
QVDWRLLAAI GYQESMWQPE VTSKTGVRGL MMLTQRTAQA MGVSNRLDPR
360 370 380 390 400
QSIQGGAKYF MKIKEELDDS IKEPDRTWFA LAAYNVGGGH LEDARTLAKR
410 420 430 440 450
EKLNPNKWLD VKKMLPRLAQ KQWYRQTKYG YARGGEPVHF VANIRRYYDI
460 470 480
LTWVTQPQLE GQVAEGNLHV PGVNKDKPAD KSSPM
Length:485
Mass (Da):55,044
Last modified:November 25, 2008 - v2
Checksum:i674FB02FB5DEDF3E
GO

Sequence cautioni

The sequence AAN66661 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66661.1 Different initiation.
RefSeqiNP_743197.2, NC_002947.4
WP_020193355.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66661; AAN66661; PP_1036
GeneIDi1044173
KEGGippu:PP_1036
PATRICifig|160488.4.peg.1099

Similar proteinsi

Entry informationi

Entry nameiMLTF_PSEPK
AccessioniPrimary (citable) accession number: Q88P17
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 25, 2008
Last sequence update: November 25, 2008
Last modified: May 23, 2018
This is version 93 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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