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Q88P16 (PUR4_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3
Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase
Gene names
Name:purL
Ordered Locus Names:PP_1037
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length1299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419

Subunit structure

Monomer.

Subcellular location

Cytoplasm HAMAP MF_00419.

Sequence similarities

In the N-terminal section; belongs to the FGAMS family.

Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentCytoplasm
   DomainGlutamine amidotransferase
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: InterPro

glutamine metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoribosylformylglycinamidine synthase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12991299Phosphoribosylformylglycinamidine synthase HAMAP MF_00419
PRO_0000100414

Regions

Domain1046 – 1299254Glutamine amidotransferase type-1
Nucleotide binding305 – 31612ATP Potential

Sites

Active site11391Nucleophile By similarity
Active site12641 By similarity
Active site12661 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88P16 [UniParc].

Last modified June 20, 2003. Version 1.
Checksum: CE3C89D988AA7EA6

FASTA1,299140,819
        10         20         30         40         50         60 
MLILRGAPAL SAFRHGKLLE QLSQKVPAVT GLYAEFAHFA DVDGELTADQ QQVLGRLLKY 

        70         80         90        100        110        120 
GPSVPVQEPT GRLFLVVPRL GTISPWASKA SDIAHNCGLQ SIQRLERGIA YYVAGTLSDA 

       130        140        150        160        170        180 
DAELIAAELH DRMTQRVLGQ LEQAADLFSH AQPKPMTSVD ILAGGRDALA KANIDLGLAL 

       190        200        210        220        230        240 
AEDEIDYLVN AFQGLKRNPN DIELMMFAQA NSEHCRHKIF NASWDIDGQA QEKSLFGMIK 

       250        260        270        280        290        300 
NTYQMHNEGV LSAYKDNASV IVGNVAGRFF PNPETRQYGA VQEPVHILMK VETHNHPTAI 

       310        320        330        340        350        360 
APFSGASTGS GGEIRDEGAT GRGAKPKAGL TGFTVSNLRI PGFEQPWEQA YGKPERIVDA 

       370        380        390        400        410        420 
LDIMIEGPLG GAAFNNEFGR PALTGYFRTF EQAINTPHGE EVRGYHKPIM LAGGMGNIRE 

       430        440        450        460        470        480 
DHVQKGEITV GAKLIVLGGP AMLIGLGGGA ASSVATGASS ADLDFASVQR ENPEMERRCQ 

       490        500        510        520        530        540 
EVIDRCWQLG DENPIAFIHD VGAGGISNAF PELVNDGGRG GRFELRNVPN DEPGMAPHEI 

       550        560        570        580        590        600 
WSNESQERYV LAVSAVDFER FKAICERERC PFAVVGEATE EQHLTVSDSH FGNTPVDMPL 

       610        620        630        640        650        660 
DVLLGKPPRM HRSVTREAEL GDDFDPSELD LDSAVQRVLN HPAVASKSFL ITIGDRTITG 

       670        680        690        700        710        720 
LVARDQMVGP WQVPVADCAV TATSFDVYTG EAMAMGERTP LALLDAPASG RMAIGETLTN 

       730        740        750        760        770        780 
LAASRIEKLS DIKLSANWMS AAGHPGEDAR LYDTVKAVGM ELCPELGITI PVGKDSMSMK 

       790        800        810        820        830        840 
TKWSDEGVEK SVTSPMSLII TGFAPVTDIR KTLTPQLRMD KGETDLILID LGRGKNRMGA 

       850        860        870        880        890        900 
SILAQTYGKI AAQAPDVDDA EDLKAFFAVI QGLNADGHLL AYHDRSDGGL VTTVLEMAFA 

       910        920        930        940        950        960 
GHCGLDLQLD PLTDSKGEVP AILFNEELGA VIQVRQDATP DVLAQFSAAG LGEECVAVIG 

       970        980        990       1000       1010       1020 
KPVNNAEVTI SLNGEVLFDD DRRMLQRQWA ETSYQIQRLR DNADCADQEF DLLLEEDNPG 

      1030       1040       1050       1060       1070       1080 
LSVKLGFDVN DDIAAPYIKK GVRPQVAILR EQGVNGQVEM AAAFDRAGFA AIDVHMSDIL 

      1090       1100       1110       1120       1130       1140 
AGRVDFEAFK GLVACGGFSY GDVLGAGEGW AKSALFNARA RDAFQAFFER TDSFALGVCN 

      1150       1160       1170       1180       1190       1200 
GCQMMSNLHE LIPGTEYWPH FVRNRSEQFE ARVAMVEVQK SNSIFLQGMA GSRMPIAIAH 

      1210       1220       1230       1240       1250       1260 
GEGHAEFASE EALLEADVSG CVALRYVDNH GKVTEAYPAN PNGSPRGITG LTSRDGRVTI 

      1270       1280       1290 
MMPHPERVFR AVQNSWRPDE WQEDAALMRM FRNARVWVN

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015451 Genomic DNA. Translation: AAN66662.1.
RefSeqNP_743198.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88P16.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ88P16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1044327.
GenomeReviewsGene locus PP_1037 in contig AE015451_GR.
KEGGppu:PP_1037.
NMPDRfig|160488.1.peg.1028.
PATRIC19940228. VBIPsePut30601_1100.
TIGRPP_1037.

Phylogenomic databases

eggNOGCOG0046.
HOGENOMHBG335309.
OMAVKAVGME.
PhylomeDBQ88P16.
ProtClustDBPRK05297.

Enzyme and pathway databases

BioCycPPUT160488:PP_1037-MONOMER.

Family and domain databases

HAMAPMF_00419. PurL_1.
[Tree]
InterProIPR000728. AIR_synth.
IPR010918. AIR_synth_C.
IPR017926. GATASE_1.
IPR010073. PRibForGlyAmidine_synth.
IPR022940. PRibForGlyAmidine_synth_bac.
IPR016188. PurM_N-like.
[Graphical view]
KOK01952.
PfamPF00586. AIRS. 1 hit.
PF02769. AIRS_C. 2 hits.
[Graphical view]
SUPFAMSSF56042. AIR_synth_C. 2 hits.
SSF55326. PurM_N-like. 2 hits.
TIGRFAMsTIGR01735. FGAM_synt. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR4_PSEPK
AccessionPrimary (citable) accession number: Q88P16
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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