Q88NX4 (OTC_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ornithine carbamoyltransferase
Short name=OTCase
EC=2.1.3.3

Gene names

Name:	argF
Ordered Locus Names:	PP_1079

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline. HAMAP MF_01109
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 1/3. HAMAP MF_01109
Subcellular location	Cytoplasm Probable HAMAP MF_01109.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	ornithine carbamoyltransferase complex Inferred from electronic annotation. Source: InterPro
Molecular function	amino acid binding Inferred from electronic annotation. Source: InterPro ornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 306	305	Ornithine carbamoyltransferase HAMAP MF_01109		PRO_0000112988
Regions
	Region	53 – 57	5	Carbamoyl phosphate binding By similarity
	Region	258 – 261	4	Ornithine binding By similarity
Sites
	Binding site	104	1	Carbamoyl phosphate By similarity
	Binding site	131	1	Carbamoyl phosphate By similarity
	Site	28	1	Important for structural integrity By similarity
	Site	144	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88NX4 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 021ED4191DFE7087	FASTA	306	34,433
	10 20 30 40 50 60 MSARHFLSLL DFTTDELLGV IRHGIELKDL RKRGVLFEPL KNRVLGMIFE KSSTRTRVSF 70 80 90 100 110 120 EAGMIQLGGQ AIFLSPRDTQ LGRGEPIGDS AIVLSSMVDV VMIRTHAHST LTEFAAKSRV 130 140 150 160 170 180 PVINGLSDES HPCQLLADMQ TFVEHRGSIQ GKTVTWIGDG FNMCNSYIEA ARQFDFQLRI 190 200 210 220 230 240 ACPEGYEPDQ RFMALGGDRV QIIRDAREAV RDAHLVVTDV WTSMGQEEET ARRLAHFAPY 250 260 270 280 290 300 QVTRELLDLA APDVLFMHCL PAHRGEEISQ DLLDDPRSVA WDEAENRLHA QKALLEFLVE PAYHHA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN66704.1.
RefSeq	NP_743240.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88NX4.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88NX4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1044722.
GenomeReviews	Gene locus PP_1079 in contig AE015451_GR.
KEGG	ppu:PP_1079.
NMPDR	fig|160488.1.peg.1070.
PATRIC	19940314. VBIPsePut30601_1143.
TIGR	PP_1079.
Phylogenomic databases
eggNOG	COG0078.
HOGENOM	HBG579429.
OMA	GVDCVVT.
PhylomeDB	Q88NX4.
ProtClustDB	PRK00779.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_1079-MONOMER.
Family and domain databases
HAMAP	MF_01109. OTCase. [Tree]
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002292. Orn/put_carbamltrans. IPR024904. Orn_carbamltrans. [Graphical view]
KO	K00611.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00102. OTCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
TIGRFAMs	TIGR00658. Orni_carb_tr. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTC_PSEPK

Accession

Primary (citable) accession number: Q88NX4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 66 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program