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Q88NJ2

- RUVC_PSEPK

UniProt

Q88NJ2 - RUVC_PSEPK

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Protein
Crossover junction endodeoxyribonuclease RuvC
Gene
ruvC, PP_1215
Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Binds 1 magnesium ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi8 – 81Magnesium By similarity
Metal bindingi67 – 671Magnesium By similarity
Metal bindingi139 – 1391Magnesium By similarity
Metal bindingi142 – 1421Magnesium By similarity

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-1253-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvC (EC:3.1.22.4)
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene namesi
Name:ruvC
Ordered Locus Names:PP_1215
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 174174Crossover junction endodeoxyribonuclease RuvCUniRule annotation
PRO_0000183122Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_1215.

Structurei

3D structure databases

ProteinModelPortaliQ88NJ2.
SMRiQ88NJ2. Positions 3-152.

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
KOiK01159.
OMAiMQIKLAL.
OrthoDBiEOG6RG044.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88NJ2-1 [UniParc]FASTAAdd to Basket

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MTLILGIDPG SRITGYGVVR QTARGCEYVA SGCIRTGSGE LHERLQIVFR    50
GVSEIIAQHG PVTMGIERVF MARNADSALK LGQARGAAIV AAAEAGLEIA 100
EYSATQVKQA VAGTGGANKE QVMMMVMHLL KLTQKPQIDA SDALAIALCH 150
AHTRSSLVPH GLTTARRRGG RLRL 174
Length:174
Mass (Da):18,428
Last modified:June 1, 2003 - v1
Checksum:i7EED59ABC60B871E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN66839.1.
RefSeqiNP_743375.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN66839; AAN66839; PP_1215.
GeneIDi1045934.
KEGGippu:PP_1215.
PATRICi19940621. VBIPsePut30601_1291.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN66839.1 .
RefSeqi NP_743375.1. NC_002947.3.

3D structure databases

ProteinModelPortali Q88NJ2.
SMRi Q88NJ2. Positions 3-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160488.PP_1215.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN66839 ; AAN66839 ; PP_1215 .
GeneIDi 1045934.
KEGGi ppu:PP_1215.
PATRICi 19940621. VBIPsePut30601_1291.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
KOi K01159.
OMAi MQIKLAL.
OrthoDBi EOG6RG044.

Enzyme and pathway databases

BioCyci PPUT160488:GIXO-1253-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiRUVC_PSEPK
AccessioniPrimary (citable) accession number: Q88NJ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi