Reviewed, UniProtKB/Swiss-Prot Q88ND5 (ALGA_PSEPK)

Names and origin

Protein names

Recommended name:
    Alginate biosynthesis protein algA
Including the following 2 domains:
    1- Recommended name:
            Mannose-6-phosphate isomerase
              EC=5.3.1.8
        Alternative name(s):
            Phosphomannose isomerase
              Short name=PMI
            Phosphohexomutase
    2- Recommended name:
            Mannose-1-phosphate guanylyltransferase
              EC=2.7.7.13
        Alternative name(s):
            GDP-mannose pyrophosphorylase
              Short name=GMPP
              Short name=GMP
            GTP--mannose-1-phosphate guanylyltransferase

Gene names

Name:	algA
Ordered Locus Names:	PP_1277

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	485 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Produces a precursor for alginate polymerization. The alginate layer provides a protective barrier against host immune defenses and antibiotics By similarity.
Catalytic activity	D-mannose 6-phosphate = D-fructose 6-phosphate. GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Cofactor	Cobalt (for PMI) By similarity.
Pathway	Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; GDP-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1. Nucleotide-sugar biosynthesis; GDP-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the mannose-6-phosphate isomerase type 2 family.

Ontologies

Keywords
Biological process	Alginate biosynthesis
Ligand	Cobalt GTP-binding Nucleotide-binding
Molecular function	Isomerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	alginic acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW lipopolysaccharide biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW cobalt ion binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC mannose-6-phosphate isomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 485	485	Alginate biosynthesis protein algA		PRO_0000194250

Sequences

	Sequence		Length	Mass (Da)
	Q88ND5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 81668C8ED0E2A90C	FASTA	485	53,896
	10 20 30 40 50 60 MMIPVILSGG SGSRLWPLSR KQFPKQFLAL TGEHTLFQQT IERLVFEGMD TPIVVCNKDH 70 80 90 100 110 120 KFIVQEQLAA LKLETQGILM EPFGRNTAPA VAMAAMKLVN EGRDELMLVL PADHVIDDQK 130 140 150 160 170 180 ALQRALALAT VAAERGEMVL FGVPATKPET GYGYIRSSQD ALLPEGVARV AQFVEKPDEK 190 200 210 220 230 240 RAAEFVQAGG YFWNSGMFLF RASRFLEELK KHDGDIYDTC VLALERSQED GDVLSIDEAT 250 260 270 280 290 300 FACCPDNSID YAVMEKTQRA CVVPMSAGWS DVGCWSSLWE VHEKDDNGNV TKGDVVVQDS 310 320 330 340 350 360 RNCMIHGNGK LVSVIGLENI VVVETKDAMM IAHKDKVQGV KQMVKTLDEQ GRTETQNHLE 370 380 390 400 410 420 VYRPWGSYDS VDMGGRFQVK HITVKPGASL SLQMHHHRAE HWIVVSGTAE VTCDENVFLL 430 440 450 460 470 480 TENQSTYIPI ASVHRLRNPG KIPLEIIEVQ SGSYLGEDDI ERFEDVYGRT STPIERGVSV KTIAQ

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66901.1.
RefSeq	NP_743437.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1043792.
GenomeReviews	Gene locus PP_1277 in contig AE015451_GR.
KEGG	ppu:PP_1277.
NMPDR	fig|160488.1.peg.1267.
TIGR	PP_1277.
Phylogenomic databases
HOGENOM	Q88ND5.
OMA	Q88ND5. VRFEDIY.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_1277-MON.
Family and domain databases
InterPro	IPR006375. Man1P_GuaTrfase/Man6P_Isoase. IPR001538. Man6P_isomerase-2_C. IPR005835. NTP_transferase. IPR014710. RmlC-like_jellyroll. [Graphical view]
Gene3D	G3DSA:2.60.120.10. RmlC-like_jellyroll. 1 hit.
Pfam	PF01050. MannoseP_isomer. 1 hit. PF00483. NTP_transferase. 1 hit. [Graphical view]
ProDom	PD002664. Man6P_isomerII. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01479. GMP_PMI. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ALGA_PSEPK

Accession

Primary (citable) accession number: Q88ND5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)