Reviewed, UniProtKB/Swiss-Prot Q88ND1 (ALGL_PSEPK)

Names and origin

Protein names

Recommended name:
    Alginate lyase
    EC=4.2.2.3
Alternative name(s):
    Poly(beta-D-mannuronate) lyase
    Poly(mana) alginate lyase

Gene names

Name:	algL
Ordered Locus Names:	PP_1281

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	371 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Depolymerizes alginate by cleaving the beta-1,4 glycosidic bond By similarity.
Catalytic activity	Eliminative cleavage of polysaccharides containing beta-D-mannuronate residues to give oligosaccharides with 4-deoxy-alpha-L-erythro-hex-4-enopyranuronosyl groups at their ends. HAMAP MF_00557
Subcellular location	Periplasm Potential.
Sequence similarities	Belongs to the polysaccharide lyase 5 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alginic acid catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	poly(beta-D-mannuronate) lyase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 28	28	Potential
	Chain	29 – 371	343	Alginate lyase HAMAP MF_00557		PRO_0000024920

Sequences

	Sequence		Length	Mass (Da)
	Q88ND1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C3FB331889DE4216	FASTA	371	41,529
	10 20 30 40 50 60 MRRPMTLFKR ISSPALLALA LFGGAAHAAL VPPQGYYQGI EKLKTGDGNF RCEAAPQPYT 70 80 90 100 110 120 GPLQFRSKYE GSDKARATLN AASEKAFRKS TEDITTLEKG VSKMVGQYMR DGRPAQLDCT 130 140 150 160 170 180 LTWLGTWARA GALLSTDYNH TGKSMRKWAL GSMSGSWLRL KFSNSQPLAA HQAEADLIEK 190 200 210 220 230 240 WLTRLAEQTV RDWSDLPLEK INNHSYWAAW SVMATAVATD RRDLFDWAVK EYKVGANQVD 250 260 270 280 290 300 DQGFLPNEIK RQQRALAYHN YALPPLAMIA SFAQANGVDL RAENNFALQR LGEGVLAGAR 310 320 330 340 350 360 DPSHFKARAG KKQDMTDLKV DSKYSWLEPW CALYHCVGDT LERKHDMQPF NSFRLGGDVT 370 RVYDPSAESK K

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN66905.1.
RefSeq	NP_743441.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	PL5. Polysaccharide Lyase Family 5.
Genome annotation databases
GeneID	1041918.
GenomeReviews	Gene locus PP_1281 in contig AE015451_GR.
KEGG	ppu:PP_1281.
NMPDR	fig|160488.1.peg.1271.
TIGR	PP_1281.
Phylogenomic databases
HOGENOM	Q88ND1.
OMA	Q88ND1. RSKYEGS.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_1281-MON.
Family and domain databases
HAMAP	MF_00557. [Tree]
InterPro	IPR008397. Alginate_lyase. [Graphical view]
Gene3D	G3DSA:1.50.10.110. Alginate_lyase. 1 hit.
Pfam	PF05426. Alginate_lyase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALGL_PSEPK

Accession

Primary (citable) accession number: Q88ND1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)