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Protein

GDP-mannose 6-dehydrogenase

Gene

algD

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of guanosine diphospho-D-mannose (GDP-D-mannose) to GDP-D-mannuronic acid, a precursor for alginate polymerization. The alginate layer causes a mucoid phenotype and provides a protective barrier against host immune defenses and antibiotics (By similarity).By similarity

Catalytic activityi

GDP-D-mannose + 2 NAD+ + H2O = GDP-D-mannuronate + 2 NADH.

Pathway: alginate biosynthesis

This protein is involved in the pathway alginate biosynthesis, which is part of Glycan biosynthesis.
View all proteins of this organism that are known to be involved in the pathway alginate biosynthesis and in Glycan biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101NAD bindingBy similarity
Sitei11 – 111NAD bindingBy similarity
Sitei30 – 301NAD bindingBy similarity
Sitei35 – 351NAD bindingBy similarity
Sitei86 – 861NAD bindingBy similarity
Sitei161 – 1611NAD bindingBy similarity
Active sitei268 – 2681By similarity
Sitei271 – 2711NAD bindingBy similarity
Sitei331 – 3311NAD bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Alginate biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-1328-MONOMER.
UniPathwayiUPA00286.

Names & Taxonomyi

Protein namesi
Recommended name:
GDP-mannose 6-dehydrogenase (EC:1.1.1.132)
Short name:
GMD
Gene namesi
Name:algD
Ordered Locus Names:PP_1288
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 438438GDP-mannose 6-dehydrogenasePRO_0000074068Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_1288.

Structurei

3D structure databases

ProteinModelPortaliQ88NC4.
SMRiQ88NC4. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
KOiK00066.
OMAiLELNYIE.
OrthoDBiEOG6ZSP7N.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88NC4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRISIFGLGY VGAVCAGCLT ARGHEVIGVD VSSTKIDLIN QGKSPIVEPG
60 70 80 90 100
LEALLQQGIA NGRLRGTTDF AEAIRASDVS MICVGTPSKK NGDLGLEYIE
110 120 130 140 150
SVCREIGYVL RDTTRRHTIV VRSTVLPGTV KNVVIPILED CSGKKAGVDF
160 170 180 190 200
GVAVNPEFLR ESTAIKDYDQ PPMTVIGELD SASGDILQAL YEELDAPIIR
210 220 230 240 250
KPIEVAEMIK YTCNVWHATK VTFANEIGNI AKAVGVDGRE VMDVVCQDKV
260 270 280 290 300
LNLSQYYMRP GFAFGGSCLP KDVRALTYRA ASLDVRAPLL DSLMRSNESQ
310 320 330 340 350
VQNAFELIEA HDKRKVALLG LSFKAGTDDL RESPLVELAE RLIGKGYQLD
360 370 380 390 400
IYDENVQYAR VHGANKDYIE SKIPHVSSLL NANLQQVIDN ADIIVLGNRD
410 420 430
EQFRALALQA PAGKQVIDLV GFMNKPTSTT ARTEGICW
Length:438
Mass (Da):47,728
Last modified:June 1, 2003 - v1
Checksum:i51CEBCDBEE95B748
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66912.1.
RefSeqiNP_743448.1. NC_002947.3.
WP_010952417.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN66912; AAN66912; PP_1288.
GeneIDi1041882.
KEGGippu:PP_1288.
PATRICi19940773. VBIPsePut30601_1365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66912.1.
RefSeqiNP_743448.1. NC_002947.3.
WP_010952417.1. NC_002947.3.

3D structure databases

ProteinModelPortaliQ88NC4.
SMRiQ88NC4. Positions 1-438.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_1288.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN66912; AAN66912; PP_1288.
GeneIDi1041882.
KEGGippu:PP_1288.
PATRICi19940773. VBIPsePut30601_1365.

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
KOiK00066.
OMAiLELNYIE.
OrthoDBiEOG6ZSP7N.

Enzyme and pathway databases

UniPathwayiUPA00286.
BioCyciPPUT160488:GIXO-1328-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR028358. GDPman_DH.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500135. GDPman_DH. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiALGD_PSEPK
AccessioniPrimary (citable) accession number: Q88NC4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: March 4, 2015
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.