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Q88NA1 (SYW_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:PP_1311
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136663

Regions

Motif11 – 199"HIGH" region HAMAP-Rule MF_00140
Motif204 – 2085"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2071ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88NA1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2A1135E6EF4F1E7C

FASTA44949,298
        10         20         30         40         50         60 
MTTRILTGIT TTGTPHLGNY AGAIRPAIRA SQQPGVDSFY FLADYHALIK CDDPLRIQRS 

        70         80         90        100        110        120 
RLEIAATWLA GGLDPDKVTF YRQSDIPEIP ELTWLLTCVA AKGLLNRAHA YKASVDKNVE 

       130        140        150        160        170        180 
NGEDPDAGVT MGLFSYPVLM AADILMFNAN KVPVGRDQIQ HVEMARDIGQ RFNHLFGQGK 

       190        200        210        220        230        240 
DFFALPEAVI EESVATLPGL DGRKMSKSYD NTIPLFTSAK DMKDAISRIV TDSRAPGEAK 

       250        260        270        280        290        300 
DPDNAHLFTL YQAFSTPEQC AGFREELLQG LGWGDAKQRL FQLLDGQLAE KREYYHQLIA 

       310        320        330        340        350        360 
RPADLEDILL AGAAKARKIA TPFLEQLREA VGLRSFRSSV QATAEVKKKA TKSARFVSFR 

       370        380        390        400        410        420 
DEDGSFRFRL LAADGEQLLL SRSFADGKSA GAVSKQLQQG GEADVRIEGL SFGLWLNGEQ 

       430        440 
VADGPQFDNA EARDAAIQSL NEALAPQQD 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015451 Genomic DNA. Translation: AAN66935.1.
RefSeqNP_743471.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88NA1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160488.PP_1311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN66935; AAN66935; PP_1311.
GeneID1041624.
KEGGppu:PP_1311.
PATRIC19940823. VBIPsePut30601_1390.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0180.
HOGENOMHOG000059939.
KOK01867.
OMALLNCYTY.
OrthoDBEOG686NJQ.

Enzyme and pathway databases

BioCycPPUT160488:GIXO-1351-MONOMER.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYW_PSEPK
AccessionPrimary (citable) accession number: Q88NA1
Entry history
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries