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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei143L-tryptophanUniRule annotation1
Binding sitei197ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 12ATPUniRule annotation3
Nucleotide bindingi18 – 19ATPUniRule annotation2
Nucleotide bindingi155 – 157ATPUniRule annotation3
Nucleotide bindingi204 – 208ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1398-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:PP_1311
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366631 – 449Tryptophan--tRNA ligaseAdd BLAST449

Proteomic databases

PRIDEiQ88NA1

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_1311

Structurei

3D structure databases

ProteinModelPortaliQ88NA1
SMRiQ88NA1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 19"HIGH" regionUniRule annotation9
Motifi204 – 208"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059939
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
IPR036913 YegP-like_sf
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
SUPFAMiSSF160113 SSF160113, 1 hit
TIGRFAMsiTIGR00233 trpS, 1 hit

Sequencei

Sequence statusi: Complete.

Q88NA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRILTGIT TTGTPHLGNY AGAIRPAIRA SQQPGVDSFY FLADYHALIK
60 70 80 90 100
CDDPLRIQRS RLEIAATWLA GGLDPDKVTF YRQSDIPEIP ELTWLLTCVA
110 120 130 140 150
AKGLLNRAHA YKASVDKNVE NGEDPDAGVT MGLFSYPVLM AADILMFNAN
160 170 180 190 200
KVPVGRDQIQ HVEMARDIGQ RFNHLFGQGK DFFALPEAVI EESVATLPGL
210 220 230 240 250
DGRKMSKSYD NTIPLFTSAK DMKDAISRIV TDSRAPGEAK DPDNAHLFTL
260 270 280 290 300
YQAFSTPEQC AGFREELLQG LGWGDAKQRL FQLLDGQLAE KREYYHQLIA
310 320 330 340 350
RPADLEDILL AGAAKARKIA TPFLEQLREA VGLRSFRSSV QATAEVKKKA
360 370 380 390 400
TKSARFVSFR DEDGSFRFRL LAADGEQLLL SRSFADGKSA GAVSKQLQQG
410 420 430 440
GEADVRIEGL SFGLWLNGEQ VADGPQFDNA EARDAAIQSL NEALAPQQD
Length:449
Mass (Da):49,298
Last modified:June 1, 2003 - v1
Checksum:i2A1135E6EF4F1E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN66935.1
RefSeqiNP_743471.1, NC_002947.4
WP_010952434.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN66935; AAN66935; PP_1311
GeneIDi1041624
KEGGippu:PP_1311
PATRICifig|160488.4.peg.1390

Similar proteinsi

Entry informationi

Entry nameiSYW_PSEPK
AccessioniPrimary (citable) accession number: Q88NA1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 92 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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