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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei207 – 2071ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-1351-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:PP_1311
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Tryptophan--tRNA ligasePRO_0000136663Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_1311.

Structurei

3D structure databases

ProteinModelPortaliQ88NA1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 199"HIGH" region
Motifi204 – 2085"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88NA1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRILTGIT TTGTPHLGNY AGAIRPAIRA SQQPGVDSFY FLADYHALIK
60 70 80 90 100
CDDPLRIQRS RLEIAATWLA GGLDPDKVTF YRQSDIPEIP ELTWLLTCVA
110 120 130 140 150
AKGLLNRAHA YKASVDKNVE NGEDPDAGVT MGLFSYPVLM AADILMFNAN
160 170 180 190 200
KVPVGRDQIQ HVEMARDIGQ RFNHLFGQGK DFFALPEAVI EESVATLPGL
210 220 230 240 250
DGRKMSKSYD NTIPLFTSAK DMKDAISRIV TDSRAPGEAK DPDNAHLFTL
260 270 280 290 300
YQAFSTPEQC AGFREELLQG LGWGDAKQRL FQLLDGQLAE KREYYHQLIA
310 320 330 340 350
RPADLEDILL AGAAKARKIA TPFLEQLREA VGLRSFRSSV QATAEVKKKA
360 370 380 390 400
TKSARFVSFR DEDGSFRFRL LAADGEQLLL SRSFADGKSA GAVSKQLQQG
410 420 430 440
GEADVRIEGL SFGLWLNGEQ VADGPQFDNA EARDAAIQSL NEALAPQQD
Length:449
Mass (Da):49,298
Last modified:June 1, 2003 - v1
Checksum:i2A1135E6EF4F1E7C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66935.1.
RefSeqiNP_743471.1. NC_002947.3.
WP_010952434.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN66935; AAN66935; PP_1311.
GeneIDi1041624.
KEGGippu:PP_1311.
PATRICi19940823. VBIPsePut30601_1390.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN66935.1.
RefSeqiNP_743471.1. NC_002947.3.
WP_010952434.1. NC_002947.3.

3D structure databases

ProteinModelPortaliQ88NA1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_1311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN66935; AAN66935; PP_1311.
GeneIDi1041624.
KEGGippu:PP_1311.
PATRICi19940823. VBIPsePut30601_1390.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-1351-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiSYW_PSEPK
AccessioniPrimary (citable) accession number: Q88NA1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 24, 2003
Last sequence update: June 1, 2003
Last modified: April 29, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.