Reviewed,
UniProtKB/Swiss-Prot Q88N74 (DDLB_PSEPK)
Last modified
February 9, 2010.
Version 51.
History...
Clusters with 100%,
90%,
50% identity |
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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents
Names and origin
| Protein names | Recommended name: D-alanine--D-alanine ligase B EC=6.3.2.4 Alternative name(s): D-alanylalanine synthetase B D-Ala-D-Ala ligase B | ||||
| Gene names |
| ||||
| Organism | Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP] | ||||
| Taxonomic identifier | 160488 [NCBI] | ||||
| Taxonomic lineage | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas |
Protein attributes
| Sequence length | 318 AA. |
| Sequence status | Complete. |
| Protein existence | Inferred from homology. |
General annotation (Comments)
| Function | Cell wall formation By similarity. HAMAP MF_00047 |
| Catalytic activity | ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP MF_00047 |
| Cofactor | Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP MF_00047 |
| Pathway | Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00047 |
| Subcellular location | Cytoplasm By similarity HAMAP MF_00047. |
| Sequence similarities | Belongs to the D-alanine--D-alanine ligase family. Contains 1 ATP-grasp domain. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 318 | 318 | D-alanine--D-alanine ligase B HAMAP MF_00047 | PRO_0000177858 | |||||
Regions | |||||||||
| Domain | 116 – 311 | 196 | ATP-grasp | ||||||
| Nucleotide binding | 142 – 197 | 56 | ATP By similarity | ||||||
Sites | |||||||||
| Metal binding | 265 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 278 | 1 | Magnesium or manganese 1 By similarity | ||||||
| Metal binding | 278 | 1 | Magnesium or manganese 2 By similarity | ||||||
| Metal binding | 280 | 1 | Magnesium or manganese 2 By similarity | ||||||
Sequences
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References
| [1] | "Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. Fraser C.M.Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | AE015451 Genomic DNA. Translation: AAN66962.1. |
| RefSeq | NP_743498.1. |
3D structure databases | |
| SMR | Q88N74. Positions 18-311. |
| ModBase | Search... |
Protein-protein interaction databases | |
| STRING | Q88N74. |
Genome annotation databases | |
| GeneID | 1044021. |
| GenomeReviews | Gene locus PP_1339 in contig AE015451_GR. |
| KEGG | ppu:PP_1339. |
| NMPDR | fig|160488.1.peg.1328. |
| TIGR | PP_1339. |
Phylogenomic databases | |
| eggNOG | COG1181. |
| HOGENOM | HBG427092. |
| OMA | RAFIILH. |
| PhylomeDB | Q88N74. |
Family and domain databases | |
| HAMAP | MF_00047. Dala_Dala_lig. [Tree] |
| InterPro | IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR000291. D-Ala_lig_Van_CS. IPR005905. D_ala_D_ala. IPR011095. Dala_Dala_lig_C. IPR011127. Dala_Dala_lig_N. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. [Graphical view] |
| Gene3D | G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit. |
| Pfam | PF07478. Dala_Dala_lig_C. 1 hit. PF01820. Dala_Dala_lig_N. 1 hit. [Graphical view] |
| TIGRFAMs | TIGR01205. D_ala_D_alaTIGR. 1 hit. |
| PROSITE | PS50975. ATP_GRASP. 1 hit. PS00843. DALA_DALA_LIGASE_1. 1 hit. PS00844. DALA_DALA_LIGASE_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Entry information
| Entry name | DDLB_PSEPK | ||||||||
| Accession | Primary (citable) accession number: Q88N74 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes) | ||||||||
Relevant documents
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

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