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Q88N31 (TTGB_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Probable efflux pump membrane transporter TtgB
Gene names
Name:ttgB
Ordered Locus Names:PP_1385
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length1050 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Probable membrane transporter component of the TtgABC efflux pump with unknown specificity.

Subcellular location

Cell inner membrane; Multi-pass membrane protein Potential.

Sequence similarities

Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family. [View classification]

Caution

There are 4 nearly identical operons in various strains of P.putida. The ttgABC operon of strain DOT-T1E and the mepABC operon of strain KT2442-TOL function in solvent and antibiotic efflux; however in strain S12 the arpABC operon functions only in antibiotic efflux. This may be due to different protein expression levels. In KT2400 this operon does not seem to function in toluene efflux.

Ontologies

Keywords
   Biological processTransport
   Cellular componentCell inner membrane
Cell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functiontransporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 10501050Probable efflux pump membrane transporter TtgB
PRO_0000161848

Regions

Transmembrane10 – 3021Helical; Potential
Transmembrane339 – 35921Helical; Potential
Transmembrane370 – 39021Helical; Potential
Transmembrane393 – 41321Helical; Potential
Transmembrane440 – 46021Helical; Potential
Transmembrane472 – 49221Helical; Potential
Transmembrane539 – 55921Helical; Potential
Transmembrane871 – 89121Helical; Potential
Transmembrane893 – 91321Helical; Potential
Transmembrane923 – 94321Helical; Potential
Transmembrane972 – 99221Helical; Potential
Transmembrane1004 – 102421Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q88N31 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: B2F0872BE50A0E53

FASTA1,050112,836
        10         20         30         40         50         60 
MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT 

        70         80         90        100        110        120 
VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ 

       130        140        150        160        170        180 
EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA 

       190        200        210        220        230        240 
QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL 

       250        260        270        280        290        300 
QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL 

       310        320        330        340        350        360 
DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ 

       370        380        390        400        410        420 
NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM 

       430        440        450        460        470        480 
SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL 

       490        500        510        520        530        540 
SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGAILRNKVP 

       550        560        570        580        590        600 
FLLAYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK 

       610        620        630        640        650        660 
DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA 

       670        680        690        700        710        720 
MVFAFAPPAV LELGNATGFD VFLQDRGGVG HEKLMEARNQ FLAKAAQSKI LSAVRPNGLN 

       730        740        750        760        770        780 
DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS 

       790        800        810        820        830        840 
PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA 

       850        860        870        880        890        900 
EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV 

       910        920        930        940        950        960 
LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA 

       970        980        990       1000       1010       1020 
AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP 

      1030       1040       1050 
LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ

« Hide

References

« Hide 'large scale' references
[1]"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440."
Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. expand/collapse author list , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.
Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: KT2440.
[2]"Comparative genomic analysis of solvent extrusion pumps in Pseudomonas strains exhibiting different degrees of solvent tolerance."
Segura A., Rojas A., Hurtado A., Huertas M.J., Ramos J.L.
Extremophiles 7:371-376(2003) [PubMed: 12743835] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015451 Genomic DNA. Translation: AAN67008.1.
RefSeqNP_743544.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88N31.
SMRQ88N31. Positions 4-1016.
ModBaseSearch...

Protein-protein interaction databases

STRINGQ88N31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1041744.
GenomeReviewsGene locus PP_1385 in contig AE015451_GR.
KEGGppu:PP_1385.
NMPDRfig|160488.1.peg.1374.
PATRIC19940981. VBIPsePut30601_1469.
TIGRPP_1385.

Phylogenomic databases

eggNOGCOG0841.
HOGENOMHBG293864.
OMAWVPLFFV.
PhylomeDBQ88N31.
ProtClustDBCLSK865853.

Enzyme and pathway databases

BioCycPPUT160488:PP_1385-MONOMER.

Family and domain databases

InterProIPR001036. Acrflvin-R.
IPR004764. HAE1.
[Graphical view]
KOK03296.
PfamPF00873. ACR_tran. 1 hit.
[Graphical view]
PRINTSPR00702. ACRIFLAVINRP.
TIGRFAMsTIGR00915. 2A0602. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTTGB_PSEPK
AccessionPrimary (citable) accession number: Q88N31
Entry history
Integrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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