Q88MX8 (CMOB_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (mo5U34)-methyltransferase
EC=2.1.1.-

Gene names

Name:	cmoB
Ordered Locus Names:	PP_1440

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	318 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA By similarity. HAMAP-Rule MF_01590
Sequence similarities	Belongs to the methyltransferase superfamily. CmoB family.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	tRNA wobble uridine modification Inferred from electronic annotation. Source: InterPro
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP tRNA (uracil) methyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 318	318	tRNA (mo5U34)-methyltransferase HAMAP-Rule MF_01590		PRO_0000313949

Sequences

	Sequence		Length	Mass (Da)
	Q88MX8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 513CAE5635DA14B2	FASTA	318	36,056
	10 20 30 40 50 60 MIDLSPLVRR LAGTPLASWS QGLQAQLDAK LEKGHGDLDR WRGALEALPA LQPSEIDLVN 70 80 90 100 110 120 GLRLDCDCDD ATRAQMRQAL MGLSPWRKGP FDLFGVHVDT EWRSDWKWSR VGPHLDLQGK 130 140 150 160 170 180 RVLDVGCGNG YYQWRMLGAG ADMVIGVDPN WLFFCQFQAV QQYLPELPAW HLPFALEDLP 190 200 210 220 230 240 ANLEGFDTVF SMGVFYHRRS PIEHLLALKD CLVKGGELVL ETLVIEGDEN QVLVPEDRYA 250 260 270 280 290 300 QMRNVWYLPS VPALARWLRR AGFSDVRCVD VSVTSVEEQR STDWMRYQSL SDFLDPNDHS 310 KTVEGLPAPR RATLLARK

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN67062.1.
RefSeq	NP_743598.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88MX8.
ModBase	Search...
Protein-protein interaction databases
STRING	160488.PP_1440.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN67062; AAN67062; PP_1440.
GeneID	1045833.
KEGG	ppu:PP_1440.
PATRIC	19941102. VBIPsePut30601_1528.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0500.
HOGENOM	HOG000218456.
KO	K15257.
OMA	WRKGPFE.
ProtClustDB	PRK15068.
Enzyme and pathway databases
BioCyc	PPUT160488:GIXO-1441-MONOMER.
Family and domain databases
HAMAP	MF_01590. tRNA_methyltr_CmoB.
InterPro	IPR010017. tRNA_mo5U34_MeTrfase. [Graphical view]
Pfam	PF08003. Methyltransf_9. 1 hit. [Graphical view]
TIGRFAMs	TIGR00452. TIGR00452. 1 hit.
ProtoNet	Search...

Entry information

Entry name

CMOB_PSEPK

Accession

Primary (citable) accession number: Q88MX8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program