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Q88MI2

- GLND_PSEPK

UniProt

Q88MI2 - GLND_PSEPK

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Protein

Bifunctional uridylyltransferase/uridylyl-removing enzyme

Gene
glnD, PP_1589
Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Modifies, by uridylylation and deuridylylation, the PII regulatory proteins (GlnB and homologs), in response to the nitrogen status of the cell that GlnD senses through the glutamine level. Under low glutamine levels, catalyzes the conversion of the PII proteins and UTP to PII-UMP and PPi, while under higher glutamine levels, GlnD hydrolyzes PII-UMP to PII and UMP (deuridylylation). Thus, controls uridylylation state and activity of the PII proteins, and plays an important role in the regulation of nitrogen assimilation and metabolism By similarity.UniRule annotation

Catalytic activityi

UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII].UniRule annotation
Uridylyl-[protein-PII] + H2O = UMP + [protein-PII].UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Uridylyltransferase (UTase) activity is inhibited by glutamine, while glutamine activates uridylyl-removing (UR) activity By similarity.UniRule annotation

GO - Molecular functioni

  1. [protein-PII] uridylyltransferase activity Source: UniProtKB-HAMAP
  2. amino acid binding Source: InterPro
  3. metal ion binding Source: InterPro
  4. phosphoric diester hydrolase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. nitrogen compound metabolic process Source: InterPro
  2. regulation of nitrogen utilization Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-1630-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional uridylyltransferase/uridylyl-removing enzyme
Short name:
UTase/UR
Alternative name(s):
Bifunctional [protein-PII] modification enzyme
Bifunctional nitrogen sensor protein
Including the following 2 domains:
[Protein-PII] uridylyltransferase (EC:2.7.7.59)
Short name:
PII uridylyltransferase
Short name:
UTase
[Protein-PII]-UMP uridylyl-removing enzyme (EC:3.1.4.-)
Short name:
UR
Gene namesi
Name:glnD
Ordered Locus Names:PP_1589
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
ProteomesiUP000000556: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 900900Bifunctional uridylyltransferase/uridylyl-removing enzymeUniRule annotationPRO_0000192754Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_1589.

Structurei

3D structure databases

ProteinModelPortaliQ88MI2.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini481 – 590110HDAdd
BLAST
Domaini706 – 78479ACT 1Add
BLAST
Domaini816 – 90085ACT 2Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 342342UridylyltransferaseUniRule annotationAdd
BLAST
Regioni343 – 705363Uridylyl-removingUniRule annotationAdd
BLAST

Domaini

Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to have a role in glutamine sensing By similarity.UniRule annotation

Sequence similaritiesi

Belongs to the GlnD family.
Contains 2 ACT domains.
Contains 1 HD domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG2844.
HOGENOMiHOG000261778.
KOiK00990.
OMAiHHLLMSV.
OrthoDBiEOG6CCH44.

Family and domain databases

Gene3Di1.10.3210.10. 1 hit.
HAMAPiMF_00277. PII_uridylyl_transf.
InterProiIPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view]
PfamiPF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view]
PIRSFiPIRSF006288. PII_uridyltransf. 1 hit.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01693. UTase_glnD. 1 hit.
PROSITEiPS51671. ACT. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88MI2-1 [UniParc]FASTAAdd to Basket

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MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRLAGEVLD KRFRAGSDIR    50
PLIEARAWLV DNILQQAWNQ FDWGDQSGIA LVAVGGYGRG ELHPHSDIDL 100
LILLGAAEHE QYRDAIERFL TLLWDIGLEV GQSVRTVDEC AEQARADLTV 150
ITNLMESRTI AGPEALRQRM LEVTSTAHMW PSKEFFLAKR AELKARHHKY 200
NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL AGEGFLLESE 250
NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIATL LGYSDENPKR 300
AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADEESGST QPLNARFRLH 350
DGYIEATHPN VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI 400
DDTFRTDIRN TSLFIELFKC EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ 450
MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV SEKFPLASKL MGRLPKPELI 500
YLAGLYHDIG KGRQGDHSEI GAVDAQKFCE RHQLPAWDSR LIVWLVQNHL 550
VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 600
SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSSAL DILVREGTDP 650
DDVEQLWAQL GDDYFLKHTA ADVAWHTDAI LQQPADGGPL VLIKETTQRE 700
FEGGTQIFIY APDQHDFFAV TVAAMSQLNL NIHDARIITS SSQFTLDTYI 750
VLDNDGGSIG DNPQRVKQIR DGLTEALRNP EDYPTIIQRR VPRQLKHFDF 800
PPQVTILNDA QRPVTILEIT APDRPGLLAR LGRIFLEFDL SLQNAKIATL 850
GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQG SDTSQTRVTF 900
Length:900
Mass (Da):102,621
Last modified:June 1, 2003 - v1
Checksum:i92BE2D95BB745A7A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN67210.1.
RefSeqiNP_743746.1. NC_002947.3.

Genome annotation databases

EnsemblBacteriaiAAN67210; AAN67210; PP_1589.
GeneIDi1045160.
KEGGippu:PP_1589.
PATRICi19941404. VBIPsePut30601_1680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AE015451 Genomic DNA. Translation: AAN67210.1 .
RefSeqi NP_743746.1. NC_002947.3.

3D structure databases

ProteinModelPortali Q88MI2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 160488.PP_1589.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAN67210 ; AAN67210 ; PP_1589 .
GeneIDi 1045160.
KEGGi ppu:PP_1589.
PATRICi 19941404. VBIPsePut30601_1680.

Phylogenomic databases

eggNOGi COG2844.
HOGENOMi HOG000261778.
KOi K00990.
OMAi HHLLMSV.
OrthoDBi EOG6CCH44.

Enzyme and pathway databases

BioCyci PPUT160488:GIXO-1630-MONOMER.

Family and domain databases

Gene3Di 1.10.3210.10. 1 hit.
HAMAPi MF_00277. PII_uridylyl_transf.
InterProi IPR002912. ACT_dom.
IPR010043. GlnD_Uridyltrans.
IPR003607. HD/PDEase_dom.
IPR006674. HD_domain.
IPR002934. Nucleotidyltransferase.
IPR013546. PII_UdlTrfase/GS_AdlTrfase.
[Graphical view ]
Pfami PF01842. ACT. 1 hit.
PF08335. GlnD_UR_UTase. 1 hit.
PF01966. HD. 1 hit.
PF01909. NTP_transf_2. 1 hit.
[Graphical view ]
PIRSFi PIRSF006288. PII_uridyltransf. 1 hit.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01693. UTase_glnD. 1 hit.
PROSITEi PS51671. ACT. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: KT2440.

Entry informationi

Entry nameiGLND_PSEPK
AccessioniPrimary (citable) accession number: Q88MI2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 7, 2003
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi