Reviewed, UniProtKB/Swiss-Prot Q88MI2 (GLND_PSEPK)

Names and origin

Protein names

Recommended name:
    [Protein-PII] uridylyltransferase
      Short name=PII uridylyl-transferase
    EC=2.7.7.59
Alternative name(s):
    Uridylyl-removing enzyme
    UTase

Gene names

Name:	glnD
Ordered Locus Names:	PP_1589

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	900 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (glnB) regulatory protein By similarity.
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP MF_00277
Sequence similarities	Belongs to the glnD family.

Ontologies

Keywords
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 900	900	[Protein-PII] uridylyltransferase HAMAP MF_00277		PRO_0000192754

Sequences

	Sequence		Length	Mass (Da)
	Q88MI2-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 92BE2D95BB745A7A	FASTA	900	102,621
	10 20 30 40 50 60 MPQVDPELFD RGQFQAELAL KASPIAAFKK AIRLAGEVLD KRFRAGSDIR PLIEARAWLV 70 80 90 100 110 120 DNILQQAWNQ FDWGDQSGIA LVAVGGYGRG ELHPHSDIDL LILLGAAEHE QYRDAIERFL 130 140 150 160 170 180 TLLWDIGLEV GQSVRTVDEC AEQARADLTV ITNLMESRTI AGPEALRQRM LEVTSTAHMW 190 200 210 220 230 240 PSKEFFLAKR AELKARHHKY NDTEYNLEPN VKGSPGGLRD IQTVLWVARR QYGTLNLHAL 250 260 270 280 290 300 AGEGFLLESE NELLASSQDF LWKVRYALHM LAGRAEDRLL FDHQRSIATL LGYSDENPKR 310 320 330 340 350 360 AIEQFMQQYY RVVMSISQLC DLIIQHFEEV ILADEESGST QPLNARFRLH DGYIEATHPN 370 380 390 400 410 420 VFKRTPFAML EIFVLMAQHP EIKGVRADTV RLLREHRHLI DDTFRTDIRN TSLFIELFKC 430 440 450 460 470 480 EIGIHRNLRR MNRYGILGRY LPEFGLIVGQ MQHDLFHIYT VDAHTLNLIK HLRKLQYTPV 490 500 510 520 530 540 SEKFPLASKL MGRLPKPELI YLAGLYHDIG KGRQGDHSEI GAVDAQKFCE RHQLPAWDSR 550 560 570 580 590 600 LIVWLVQNHL VMSTTAQRKD LSDPQVINDF ALHVGDETRL DYLYVLTVAD INATNPSLWN 610 620 630 640 650 660 SWRASLLRQL YTETKRALRR GLENPLDREE QIRQTQSSAL DILVREGTDP DDVEQLWAQL 670 680 690 700 710 720 GDDYFLKHTA ADVAWHTDAI LQQPADGGPL VLIKETTQRE FEGGTQIFIY APDQHDFFAV 730 740 750 760 770 780 TVAAMSQLNL NIHDARIITS SSQFTLDTYI VLDNDGGSIG DNPQRVKQIR DGLTEALRNP 790 800 810 820 830 840 EDYPTIIQRR VPRQLKHFDF PPQVTILNDA QRPVTILEIT APDRPGLLAR LGRIFLEFDL 850 860 870 880 890 900 SLQNAKIATL GERVEDVFFI TDADNQPLSD PQLCSRLQEA IVQQLQAGQG SDTSQTRVTF

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN67210.1.
RefSeq	NP_743746.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1045160.
GenomeReviews	Gene locus PP_1589 in contig AE015451_GR.
KEGG	ppu:PP_1589.
NMPDR	fig|160488.1.peg.1576.
TIGR	PP_1589.
Phylogenomic databases
HOGENOM	Q88MI2.
OMA	Q88MI2. MQHDLFH.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_1589-MON.
Family and domain databases
HAMAP	MF_00277. [Tree]
InterPro	IPR002912. ACT_bd. IPR010043. GlnD_Uridyltrans. IPR003607. Met-dep_phosphohydro_HD. IPR006674. Met-dep_phosphohydro_HD_sub. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. GlnD_Uridyltrans. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_PSEPK

Accession

Primary (citable) accession number: Q88MI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)