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Protein

Fumarate hydratase class II

Gene

fumC

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the TCA cycle. Catalyzes the stereospecific interconversion of fumarate to L-malate.UniRule annotation

Miscellaneous

There are 2 substrate-binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors.UniRule annotation

Catalytic activityi

(S)-malate = fumarate + H2O.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from fumarate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Fumarate hydratase class II (fumC), Fumarate hydratase class II (fumC-I)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from fumarate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei185SubstrateUniRule annotation1
Active sitei186Proton donor/acceptorUniRule annotation1
Active sitei316UniRule annotation1
Binding sitei317SubstrateUniRule annotation1
Sitei329Important for catalytic activityUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-1856-MONOMER
UniPathwayiUPA00223; UER01007

Names & Taxonomyi

Protein namesi
Recommended name:
Fumarate hydratase class IIUniRule annotation (EC:4.2.1.2UniRule annotation)
Short name:
Fumarase CUniRule annotation
Alternative name(s):
Aerobic fumaraseUniRule annotation
Iron-independent fumaraseUniRule annotation
Gene namesi
Name:fumCUniRule annotation
Synonyms:fumC-2
Ordered Locus Names:PP_1755
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001613011 – 464Fumarate hydratase class IIAdd BLAST464

Proteomic databases

PRIDEiQ88M20

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_1755

Structurei

3D structure databases

ProteinModelPortaliQ88M20
SMRiQ88M20
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni96 – 98Substrate bindingUniRule annotation3
Regioni127 – 130Substrate binding (B site)UniRule annotation4
Regioni137 – 139Substrate bindingUniRule annotation3
Regioni322 – 324Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C9Q Bacteria
COG0114 LUCA
HOGENOMiHOG000061736
KOiK01679
OMAiVSFTDNC

Family and domain databases

CDDicd01362 Fumarase_classII, 1 hit
Gene3Di1.10.275.10, 1 hit
HAMAPiMF_00743 FumaraseC, 1 hit
InterProiView protein in InterPro
IPR005677 Fum_hydII
IPR024083 Fumarase/histidase_N
IPR018951 Fumarase_C_C
IPR020557 Fumarate_lyase_CS
IPR000362 Fumarate_lyase_fam
IPR022761 Fumarate_lyase_N
IPR008948 L-Aspartase-like
PANTHERiPTHR11444 PTHR11444, 1 hit
PfamiView protein in Pfam
PF10415 FumaraseC_C, 1 hit
PF00206 Lyase_1, 1 hit
PRINTSiPR00149 FUMRATELYASE
SUPFAMiSSF48557 SSF48557, 1 hit
TIGRFAMsiTIGR00979 fumC_II, 1 hit
PROSITEiView protein in PROSITE
PS00163 FUMARATE_LYASES, 1 hit

Sequencei

Sequence statusi: Complete.

Q88M20-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRIETDSLG PVEVPEDAYW GAQTQRSLIN FAIGKERMPL AVLHALALIK
60 70 80 90 100
KAAARVNDRN GDLPADIARL IEQAADEVLD GQHDDQFPLV VWQTGSGTQS
110 120 130 140 150
NMNVNEVIAG RANELAGKGR GGKAPVHPND HVNRSQSSND CFPTAMHIAA
160 170 180 190 200
AQAVHEKLLP AVTELSSGLA ELSMRHHKLV KTGRTHMMDA TPITFGQEVS
210 220 230 240 250
AFVAQLDYAQ RAIRATLPAV CELAQGGTAV GTGLNAPQGF AEAIAAELAA
260 270 280 290 300
LSGLPFITAP NKFAALAGHE PLTSLAGALK TLAVALMKIA NDLRLLGSGP
310 320 330 340 350
RAGLAEVRLP ANEPGSSIMP GKVNPTQCEA LSMLACQVLG NDAAIGFAAS
360 370 380 390 400
QGHLQLNVFK PVIIHNLLQS IELLADGCRN FQQHCVAGIE PDAEQMAAHL
410 420 430 440 450
ERGLMLVTAL NPHIGYDKAA EIAKKAYSEG TTLREAALAL KYLTNEQFDQ
460
WVRPENMLAP GGKG
Length:464
Mass (Da):49,131
Last modified:June 1, 2003 - v1
Checksum:i3501E4688552BF4D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN67375.1
RefSeqiNP_743911.1, NC_002947.4
WP_010952795.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN67375; AAN67375; PP_1755
GeneIDi1043414
KEGGippu:PP_1755
PATRICifig|160488.4.peg.1850

Similar proteinsi

Entry informationi

Entry nameiFUMC_PSEPK
AccessioniPrimary (citable) accession number: Q88M20
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 98 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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