Reviewed, UniProtKB/Swiss-Prot Q88LX0 (KDSA2_PSEPK)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase 2
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase 2
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase 2
    KDO-8-phosphate synthetase 2
      Short name=KDO 8-P synthase 2
      Short name=KDOPS 2

Gene names

Name:	kdsA2
Synonyms:	kdsA-2
Ordered Locus Names:	PP_1807

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	280 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity HAMAP MF_00056.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 280	280	2-dehydro-3-deoxyphosphooctonate aldolase 2 HAMAP MF_00056		PRO_0000187152

Sequences

	Sequence		Length	Mass (Da)
	Q88LX0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 1300418DC9803F4E	FASTA	280	30,469
	10 20 30 40 50 60 MIKINSTIEC SNSAPFVLFG GINVLESEDL ALTACAEYVR VTQKLGIPYV FKASFDKANR 70 80 90 100 110 120 SSIHSYRGPG MEEGLRIFEK VKAEFGVPII TDVHEIYQTA PVAEVVDVLQ LPAFLARQTD 130 140 150 160 170 180 LVVALAKTGK PVNIKKPQFL SPSQMQNIVH KFKEAGNDQL ILCDRGTCMG YDNLIVDMLG 190 200 210 220 230 240 FGVMKRTCQD LPIIFDVTHA LQNRDPSGAA SGGRREQVVE LARAGMGVGL AGLFLEAHPN 250 260 270 280 PDQAKCDGPS ALPLDKLEPF LAQIKALDDL VKSFPQLTIA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN67426.1.
RefSeq	NP_743962.1.
3D structure databases
SMR	Q88LX0. Positions 8-277.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88LX0.
Genome annotation databases
GeneID	1043295.
GenomeReviews	Gene locus PP_1807 in contig AE015451_GR.
KEGG	ppu:PP_1807.
NMPDR	fig|160488.1.peg.1792.
TIGR	PP_1807.
Phylogenomic databases
eggNOG	COG2877.
HOGENOM	HBG575990.
OMA	QLILCDR.
PhylomeDB	Q88LX0.
Family and domain databases
HAMAP	MF_00056. KDO8P_synth. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA2_PSEPK

Accession

Primary (citable) accession number: Q88LX0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)