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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei184Thioimide intermediateUniRule annotation1
Active sitei191Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 86NADPHUniRule annotation2
Nucleotide bindingi252 – 253NADPHUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processQueuosine biosynthesis
LigandNADP

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-2301-MONOMER
UniPathwayiUPA00392

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:PP_2160
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001630461 – 276NADPH-dependent 7-cyano-7-deazaguanine reductaseAdd BLAST276

Proteomic databases

PRIDEiQ88KX9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_2160

Structurei

3D structure databases

ProteinModelPortaliQ88KX9
SMRiQ88KX9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni83 – 85Substrate bindingUniRule annotation3
Regioni223 – 224Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1K Bacteria
COG0780 LUCA
COG2904 LUCA
HOGENOMiHOG000273755
KOiK06879
OMAiQCVERIY

Family and domain databases

HAMAPiMF_00817 QueF_type2, 1 hit
InterProiView protein in InterPro
IPR029500 QueF
IPR029139 QueF_N
IPR016428 QueF_type2
PfamiView protein in Pfam
PF14489 QueF, 1 hit
PF14819 QueF_N, 1 hit
PIRSFiPIRSF004750 Nitrile_oxidored_YqcD_prd, 1 hit
TIGRFAMsiTIGR03138 QueF, 1 hit

Sequencei

Sequence statusi: Complete.

Q88KX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPAAEHSPL GKSSEYIATY SPEQLFPIPR TAKWAELGVT AQTLPWQGVD
60 70 80 90 100
YWNCFELSWL LPSGKPVVAI GEFAIPADSP NIIESKSFKL YLNSLNQTVF
110 120 130 140 150
TSLGALQVCL EKDLSAAAGK PVGVKVRTLA EVEAQGVVAL PGQCIDALDV
160 170 180 190 200
AISNYEQPQP ELLRCNPERV VEETLHSHLL KSNCPVTGQP DWGSVVVQYK
210 220 230 240 250
GRALDHASLL TYLISFRQHA DFHEQCVERI YLDLKNLLQP EHLTVYARYV
260 270
RRGGLDINPY RSTGPISPDN KRLVRQ
Length:276
Mass (Da):30,686
Last modified:June 1, 2003 - v1
Checksum:i04A44D5229CF0CA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN67773.1
RefSeqiNP_744309.1, NC_002947.4
WP_010953147.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN67773; AAN67773; PP_2160
GeneIDi1045018
KEGGippu:PP_2160
PATRICifig|160488.4.peg.2277

Similar proteinsi

Entry informationi

Entry nameiQUEF_PSEPK
AccessioniPrimary (citable) accession number: Q88KX9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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