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Protein

NADPH-dependent 7-cyano-7-deazaguanine reductase

Gene

queF

Organism
Pseudomonas putida (strain KT2440)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1).UniRule annotation

Catalytic activityi

7-aminomethyl-7-carbaguanine + 2 NADP+ = 7-cyano-7-carbaguanine + 2 NADPH.UniRule annotation

Pathwayi: tRNA-queuosine biosynthesis

This protein is involved in the pathway tRNA-queuosine biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway tRNA-queuosine biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei184 – 1841Thioimide intermediateUniRule annotation
Active sitei191 – 1911Proton donorUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi85 – 862NADPHUniRule annotation
Nucleotide bindingi252 – 2532NADPHUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Queuosine biosynthesis

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciPPUT160488:GIXO-2223-MONOMER.
UniPathwayiUPA00392.

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH-dependent 7-cyano-7-deazaguanine reductaseUniRule annotation (EC:1.7.1.13UniRule annotation)
Alternative name(s):
7-cyano-7-carbaguanine reductaseUniRule annotation
NADPH-dependent nitrile oxidoreductaseUniRule annotation
PreQ(0) reductaseUniRule annotation
Gene namesi
Name:queFUniRule annotation
Ordered Locus Names:PP_2160
OrganismiPseudomonas putida (strain KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 276276NADPH-dependent 7-cyano-7-deazaguanine reductasePRO_0000163046Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_2160.

Structurei

3D structure databases

ProteinModelPortaliQ88KX9.
SMRiQ88KX9. Positions 16-276.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 853Substrate bindingUniRule annotation
Regioni223 – 2242Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88KX9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHPAAEHSPL GKSSEYIATY SPEQLFPIPR TAKWAELGVT AQTLPWQGVD
60 70 80 90 100
YWNCFELSWL LPSGKPVVAI GEFAIPADSP NIIESKSFKL YLNSLNQTVF
110 120 130 140 150
TSLGALQVCL EKDLSAAAGK PVGVKVRTLA EVEAQGVVAL PGQCIDALDV
160 170 180 190 200
AISNYEQPQP ELLRCNPERV VEETLHSHLL KSNCPVTGQP DWGSVVVQYK
210 220 230 240 250
GRALDHASLL TYLISFRQHA DFHEQCVERI YLDLKNLLQP EHLTVYARYV
260 270
RRGGLDINPY RSTGPISPDN KRLVRQ
Length:276
Mass (Da):30,686
Last modified:June 1, 2003 - v1
Checksum:i04A44D5229CF0CA7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN67773.1.
RefSeqiNP_744309.1. NC_002947.4.
WP_010953147.1. NC_002947.4.

Genome annotation databases

EnsemblBacteriaiAAN67773; AAN67773; PP_2160.
GeneIDi1045018.
KEGGippu:PP_2160.
PATRICi19942642. VBIPsePut30601_2277.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN67773.1.
RefSeqiNP_744309.1. NC_002947.4.
WP_010953147.1. NC_002947.4.

3D structure databases

ProteinModelPortaliQ88KX9.
SMRiQ88KX9. Positions 16-276.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_2160.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN67773; AAN67773; PP_2160.
GeneIDi1045018.
KEGGippu:PP_2160.
PATRICi19942642. VBIPsePut30601_2277.

Phylogenomic databases

eggNOGiENOG4107R1K. Bacteria.
COG0780. LUCA.
COG2904. LUCA.
HOGENOMiHOG000273755.
KOiK06879.
OMAiQCVERIY.

Enzyme and pathway databases

UniPathwayiUPA00392.
BioCyciPPUT160488:GIXO-2223-MONOMER.

Family and domain databases

HAMAPiMF_00817. QueF_type2. 1 hit.
InterProiIPR029500. QueF.
IPR029139. QueF_N.
IPR016428. QueF_type2.
[Graphical view]
PfamiPF14489. QueF. 1 hit.
PF14819. QueF_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004750. Nitrile_oxidored_YqcD_prd. 1 hit.
TIGRFAMsiTIGR03138. QueF. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQUEF_PSEPK
AccessioniPrimary (citable) accession number: Q88KX9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 22, 2005
Last sequence update: June 1, 2003
Last modified: September 7, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.