Q88K84 (AROQ2_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase 2
Short name=3-dehydroquinase 2
EC=4.2.1.10

Alternative name(s):
Type II DHQase 2

Gene names

Name:	aroQ2
Synonyms:	aroQ-2
Ordered Locus Names:	PP_2407

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	149 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a trans-dehydration via an enolate intermediate By similarity. HAMAP MF_00169
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00169
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00169
Subunit structure	Homododecamer By similarity. HAMAP MF_00169
Sequence similarities	Belongs to the type-II 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 149	149	3-dehydroquinate dehydratase 2 HAMAP MF_00169		PRO_0000159921
Regions
	Region	102 – 103	2	Substrate binding By similarity
Sites
	Active site	24	1	Proton acceptor By similarity
	Active site	101	1	Proton donor By similarity
	Binding site	75	1	Substrate By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	88	1	Substrate By similarity
	Binding site	112	1	Substrate By similarity
	Site	19	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88K84 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: D10A4D670DA9D124	FASTA	149	16,211
	10 20 30 40 50 60 MKPLILVLNG PNLNMLGTRE PAQYGHETLA DLAQGCADTA HAHGLEIEFR QTNHEGELID 70 80 90 100 110 120 WIHAARGRCA GIVINPGAWT HTSVAIRDAL VASELPVIEV HLSNVHKREP FRHLSFVSSI 130 140 AVGVICGLGS HGYRMALSHF AELLQERAA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN68019.1.
RefSeq	NP_744555.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88K84.
SMR	Q88K84. Positions 1-146.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88K84.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1045534.
GenomeReviews	Gene locus PP_2407 in contig AE015451_GR.
KEGG	ppu:PP_2407.
PATRIC	19943208. VBIPsePut30601_2550.
TIGR	PP_2407.
Phylogenomic databases
eggNOG	COG0757.
HOGENOM	HOG000217278.
KO	K03786.
OMA	EFRQTNH.
ProtClustDB	PRK13015.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_2407-MONOMER.
Family and domain databases
Gene3D	G3DSA:3.40.50.9100. DHquinase_II. 1 hit.
HAMAP	MF_00169. AroQ. [Tree]
InterPro	IPR001874. DHquinase_II. IPR018509. DHquinase_II_CS. [Graphical view]
PANTHER	PTHR21272. DHquinase_II. 1 hit.
Pfam	PF01220. DHquinase_II. 1 hit. [Graphical view]
PIRSF	PIRSF001399. DHquinase_II. 1 hit.
ProDom	PD004527. DHquinase_II. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF52304. DHquinase_II. 1 hit.
TIGRFAMs	TIGR01088. AroQ. 1 hit.
PROSITE	PS01029. DEHYDROQUINASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROQ2_PSEPK

Accession

Primary (citable) accession number: Q88K84

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	May 16, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program