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Protein

4-oxalmesaconate hydratase

Gene

galB

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxalomesaconic acid enol (OMAenol) to 4-carboxy-4-hydroxy-2-oxoadipic acid (CHA). Mediates the third step of gallate degradation pathway.1 Publication

Catalytic activityi

2-hydroxy-4-oxobutane-1,2,4-tricarboxylate = (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate + H2O.1 Publication

Cofactori

Zn2+Note: Binds 1 zinc ion per subunit.

Kineticsi

  1. KM=85 µM for (1E,3E)-4-hydroxybuta-1,3-diene-1,2,4-tricarboxylate1 Publication
  1. Vmax=76 µmol/min/mg enzyme1 Publication

pH dependencei

Optimum pH is 7.0.1 Publication

Temperature dependencei

Optimum temperature is 30 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28ZincBy similarity1
Metal bindingi31ZincBy similarity1
Metal bindingi141ZincBy similarity1

GO - Molecular functioni

  • 4-oxalmesaconate hydratase activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • aromatic compound catabolic process Source: UniProtKB-KW
  • gallate catabolic process Source: UniProtKB
  • metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16015.

Names & Taxonomyi

Protein namesi
Recommended name:
4-oxalmesaconate hydratase (EC:4.2.1.83)
Short name:
OMA hydratase
Alternative name(s):
Gallate degradation protein B
Gene namesi
Name:galB
Ordered Locus Names:PP_2515
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Lacks 4-oxalmesaconate hydratase activity.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004184971 – 2584-oxalmesaconate hydrataseAdd BLAST258

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_2515.

Structurei

Secondary structure

1258
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi21 – 28Combined sources8
Helixi31 – 45Combined sources15
Beta strandi49 – 55Combined sources7
Turni59 – 61Combined sources3
Helixi64 – 68Combined sources5
Helixi74 – 92Combined sources19
Beta strandi94 – 98Combined sources5
Beta strandi103 – 105Combined sources3
Helixi110 – 123Combined sources14
Beta strandi128 – 130Combined sources3
Helixi140 – 156Combined sources17
Beta strandi172 – 175Combined sources4
Turni180 – 184Combined sources5
Beta strandi188 – 192Combined sources5
Helixi194 – 196Combined sources3
Helixi197 – 204Combined sources8
Helixi211 – 230Combined sources20
Beta strandi243 – 250Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CGZX-ray2.10A/B20-258[»]
ProteinModelPortaliQ88JX8.
SMRiQ88JX8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MshB deacetylase family.Curated

Phylogenomic databases

eggNOGiCOG2120. LUCA.
HOGENOMiHOG000266854.
KOiK16515.
OMAiHAADFVW.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.

Sequencei

Sequence statusi: Complete.

Q88JX8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSCAHPHCR SQRNMNTPQK SALVVSAHSA DFVWRAGGAI ALHAEQGYAM
60 70 80 90 100
HVVCLSFGER GESAKLWRKG EMTEAKVKDA RREEAMAAAE ILGASVEFFD
110 120 130 140 150
IGDYPMRADK DTLFRLADVY RRVQPEFVLS HSLKDPYNYD HPLAMHLAQE
160 170 180 190 200
ARIIAQAEGY KPGEKIVGAP PVYAFEPHQP EQCEWRPDTF LDITSVWDKK
210 220 230 240 250
YAAIQCMAGQ EHLWEYYTRV ALQRGVQAKR NVGITSARNI VYAEGLQSVF

PRVTENLA
Length:258
Mass (Da):29,071
Last modified:June 1, 2003 - v1
Checksum:i40D2E5E9190C4A24
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN68127.1.
RefSeqiNP_744663.1. NC_002947.4.
WP_010953449.1. NC_002947.4.

Genome annotation databases

GeneIDi1045763.
KEGGippu:PP_2515.
PATRICi19943452. VBIPsePut30601_2670.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN68127.1.
RefSeqiNP_744663.1. NC_002947.4.
WP_010953449.1. NC_002947.4.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5CGZX-ray2.10A/B20-258[»]
ProteinModelPortaliQ88JX8.
SMRiQ88JX8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_2515.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1045763.
KEGGippu:PP_2515.
PATRICi19943452. VBIPsePut30601_2670.

Phylogenomic databases

eggNOGiCOG2120. LUCA.
HOGENOMiHOG000266854.
KOiK16515.
OMAiHAADFVW.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-16015.

Family and domain databases

Gene3Di3.40.50.10320. 1 hit.
InterProiIPR003737. GlcNAc_PI_deacetylase-related.
IPR024078. LmbE-like_dom.
[Graphical view]
PANTHERiPTHR12993. PTHR12993. 1 hit.
PfamiPF02585. PIG-L. 1 hit.
[Graphical view]
SUPFAMiSSF102588. SSF102588. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGALB_PSEPK
AccessioniPrimary (citable) accession number: Q88JX8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 5, 2012
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.