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Protein

Glutamine--tRNA ligase

Gene

glnS

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei80L-glutamineUniRule annotation1
Binding sitei225L-glutamineUniRule annotation1
Binding sitei244ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi48 – 50ATPUniRule annotation3
Nucleotide bindingi54 – 60ATPUniRule annotation7
Nucleotide bindingi274 – 275ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-3083-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligaseUniRule annotation (EC:6.1.1.18UniRule annotation)
Alternative name(s):
Glutaminyl-tRNA synthetaseUniRule annotation
Short name:
GlnRSUniRule annotation
Gene namesi
Name:glnSUniRule annotation
Ordered Locus Names:PP_2904
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001958441 – 567Glutamine--tRNA ligaseAdd BLAST567

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_2904

Structurei

3D structure databases

ProteinModelPortaliQ88IU5
SMRiQ88IU5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi47 – 57"HIGH" regionUniRule annotationAdd BLAST11
Motifi281 – 285"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CX6 Bacteria
COG0008 LUCA
HOGENOMiHOG000259232
KOiK01886
OMAiVTHSICT

Family and domain databases

Gene3Di1.10.1160.101 hit
2.40.240.103 hits
3.40.50.6202 hits
HAMAPiMF_00126 Gln_tRNA_synth, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR004514 Gln-tRNA-synth
IPR022861 Gln_tRNA_ligase_bac
IPR000924 Glu/Gln-tRNA-synth
IPR020058 Glu/Gln-tRNA-synth_Ib_cat-dom
IPR020059 Glu/Gln-tRNA-synth_Ib_codon-bd
IPR020061 Glu_tRNA_lig_a-bdl
IPR020056 Rbsml_L25/Gln-tRNA_synth_N
IPR011035 Ribosomal_L25/Gln-tRNA_synth
IPR014729 Rossmann-like_a/b/a_fold
PfamiView protein in Pfam
PF00749 tRNA-synt_1c, 1 hit
PF03950 tRNA-synt_1c_C, 1 hit
PRINTSiPR00987 TRNASYNTHGLU
SUPFAMiSSF50715 SSF50715, 1 hit
TIGRFAMsiTIGR00440 glnS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q88IU5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKPTADNAP NAAAKGAPAV PANFLRPIIQ ADLDSGKHSS IVTRFPPEPN
60 70 80 90 100
GYLHIGHAKS ICVNFGLAKE FGGVCHLRFD DTNPAKEDQE YIDAIQSDVK
110 120 130 140 150
WLGFEWGGDV RYASDYFDQL HDWAVELIKR GKAYVCDLTP EQAKEYRGNL
160 170 180 190 200
KEPGKNSPFR ERSVEENLDL FARMKAGEFK DGERVLRAKI DMASPNMNLR
210 220 230 240 250
DPILYRIRHA HHHQTGDKWC IYPNYDFTHG QSDAIEGITH SICTLEFEGH
260 270 280 290 300
RPLYDWFLDN LPVPAHPRQY EFSRLNLNYT ITSKRKLKQL VDEKHVDAWD
310 320 330 340 350
DPRMSTLSGF RRRGYTPASI RNFCEMIGTN RSDGVVDMSM LEFSIRDDLD
360 370 380 390 400
RTAPRAMCVL RPLKVVITNY PEGQVEQLEL PRHPKEDMGV RVLPFSRELY
410 420 430 440 450
IDRDDFMEEP PKGYKRLEPA GEVRLRGSYV IRADEAIKDA DGNIVELRCS
460 470 480 490 500
YDPDTLGKNP EGRKVKGVIH WVPAEGSVEC EVRLYDRLFR SPNPEKTEEG
510 520 530 540 550
GSFLDNINPA SLQVLSGCRA EPSLAQAQPE DRFQFEREGY FCADLKDSQP
560
GRPVFNRTVT LRDSWGS
Length:567
Mass (Da):64,655
Last modified:June 1, 2003 - v1
Checksum:iA6BAA822F3AD97AF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN68512.1
RefSeqiNP_745048.1, NC_002947.4
WP_010953806.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN68512; AAN68512; PP_2904
GeneIDi1042815
KEGGippu:PP_2904
PATRICifig|160488.4.peg.3078

Similar proteinsi

Entry informationi

Entry nameiSYQ_PSEPK
AccessioniPrimary (citable) accession number: Q88IU5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: February 28, 2018
This is version 95 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome