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Q88IU5 (SYQ_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:PP_2904
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length567 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 567567Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_0000195844

Regions

Motif47 – 5711"HIGH" region HAMAP-Rule MF_00126
Motif281 – 2855"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2841ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88IU5 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A6BAA822F3AD97AF

FASTA56764,655
        10         20         30         40         50         60 
MSKPTADNAP NAAAKGAPAV PANFLRPIIQ ADLDSGKHSS IVTRFPPEPN GYLHIGHAKS 

        70         80         90        100        110        120 
ICVNFGLAKE FGGVCHLRFD DTNPAKEDQE YIDAIQSDVK WLGFEWGGDV RYASDYFDQL 

       130        140        150        160        170        180 
HDWAVELIKR GKAYVCDLTP EQAKEYRGNL KEPGKNSPFR ERSVEENLDL FARMKAGEFK 

       190        200        210        220        230        240 
DGERVLRAKI DMASPNMNLR DPILYRIRHA HHHQTGDKWC IYPNYDFTHG QSDAIEGITH 

       250        260        270        280        290        300 
SICTLEFEGH RPLYDWFLDN LPVPAHPRQY EFSRLNLNYT ITSKRKLKQL VDEKHVDAWD 

       310        320        330        340        350        360 
DPRMSTLSGF RRRGYTPASI RNFCEMIGTN RSDGVVDMSM LEFSIRDDLD RTAPRAMCVL 

       370        380        390        400        410        420 
RPLKVVITNY PEGQVEQLEL PRHPKEDMGV RVLPFSRELY IDRDDFMEEP PKGYKRLEPA 

       430        440        450        460        470        480 
GEVRLRGSYV IRADEAIKDA DGNIVELRCS YDPDTLGKNP EGRKVKGVIH WVPAEGSVEC 

       490        500        510        520        530        540 
EVRLYDRLFR SPNPEKTEEG GSFLDNINPA SLQVLSGCRA EPSLAQAQPE DRFQFEREGY 

       550        560 
FCADLKDSQP GRPVFNRTVT LRDSWGS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015451 Genomic DNA. Translation: AAN68512.1.
RefSeqNP_745048.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88IU5.
SMRQ88IU5. Positions 23-564.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160488.PP_2904.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN68512; AAN68512; PP_2904.
GeneID1042815.
KEGGppu:PP_2904.
PATRIC19944266. VBIPsePut30601_3078.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OMASREIYID.
OrthoDBEOG6DRPF7.

Enzyme and pathway databases

BioCycPPUT160488:GIXO-2980-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_PSEPK
AccessionPrimary (citable) accession number: Q88IU5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries