Reviewed, UniProtKB/Swiss-Prot Q88I82 (DNAE2_PSEPK)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	PP_3119

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subcellular location	CytoplasmProbable.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: InterPro error-prone DNA repair Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1033	1033	Error-prone DNA polymerase		PRO_0000103390

Sequences

	Sequence		Length	Mass (Da)
	Q88I82-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 63F6929BED45D995	FASTA	1,033	115,631
	10 20 30 40 50 60 MAAGLVRMNT PGYAELHCLS NFSFQRGASS ADELFRRARE QGYQALAITD ECTLAGIVRA 70 80 90 100 110 120 WQAAKVHQLR LIVGSEVQLC DGPKLVLLVE NLTGYQNLCA LITRARRRAE KGAYQLFRDD 130 140 150 160 170 180 LLLHHQGLLA LWVAADSGDT ATGAWLRSVF AERLWLAVHL HRGSDDAVRL QRLRALAADV 190 200 210 220 230 240 GIRAVACGDV HMHVRGRRAL QDCMTAIRQH CQVSEAGRFL FANGERHLRS QAQLAELYPL 250 260 270 280 290 300 DLLAETLVIA RRCQFDLSEL NYQYPRELVP EGHTPASWLR ELCEQGMPLR WPDGPSGKVR 310 320 330 340 350 360 DVLAKELGLI EELGYESYFL TVHDIVAFAR SQRILCQGRG SAANSVVCFV LGITELDPMK 370 380 390 400 410 420 HHLLFERFLS RERNEPPDID VDFEHDRREE VIQYVFRRYG RHRAALTAVV NTYHAAGAVR 430 440 450 460 470 480 DVARALGLPA DQVDALAKCC GRWSDRIPDD QRLAEAGFEA GSPSLRRVLV LAGELIGFPR 490 500 510 520 530 540 HLSQHPGGFV ISQQPLDQLV PVENAAMPER TVIQWDKDDL DMVGLLKVDV LALGMLSALR 550 560 570 580 590 600 RCFDQLQHHR GRHLTLATIP SEDPATYAMI SRAETMGVFQ IESRAQMAML PRLRPQKFYD 610 620 630 640 650 660 LVIEVAIVRP GPIQGDMVHP YLRRRLKQEP VTYPSPQLKE VFERTLGVPL FQEQVMELAM 670 680 690 700 710 720 VAADYTPGEA DQLRRSMAAW KRHGGLEPHR ERLVQGMLRN GYTLAFAERI FEQIKGFGSY 730 740 750 760 770 780 GFPESHAASF ALLCYASSWL KCHEPAIFTC ALVNSWPMGF YSPDQLLQEA RRQGIEVRPV 790 800 810 820 830 840 DVCHSDWDCT LEPDAEGTLA IRMGLRLVRG LAEADAKRVQ QARSQRPWRN VEDLCLRAGL 850 860 870 880 890 900 DARARARLAD GGALRALASD RHQARWQVAA VQPQLPLFAD VQALPEEPVQ LPVPTVGEDL 910 920 930 940 950 960 MADYQTLGTT LGPHPLALLR ARLRALGCRS SSELQGVEHG DNIAVAGLVV GRQRPQTASG 970 980 990 1000 1010 1020 VTFVTLEDEH GMVNVVVWRA LAERQRRALV GSQLLKVSGR LEQENGVRHL IARRLEDVSP 1030 LLQGLDVRSR DFH

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN68727.1.
RefSeq	NP_745263.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1043951.
GenomeReviews	Gene locus PP_3119 in contig AE015451_GR.
KEGG	ppu:PP_3119.
NMPDR	fig|160488.1.peg.3093.
TIGR	PP_3119.
Phylogenomic databases
HOGENOM	Q88I82.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_3119-MON.
Family and domain databases
HAMAP	MF_01902. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. PHP_N. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_PSEPK

Accession

Primary (citable) accession number: Q88I82

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)