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Q88FQ4 (RNZ_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribonuclease Z
Short name=RNase Z
EC=3.1.26.11
Alternative name(s):
tRNA 3 endonuclease
tRNase Z
Gene names
Name:rnz
Ordered Locus Names:PP_4033
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas ›

Protein attributes

Sequence length319 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA By similarity. HAMAP-Rule MF_01818

Catalytic activity

Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule. HAMAP-Rule MF_01818

Cofactor

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the RNase Z family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandMetal-binding
Zinc
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtRNA 3'-trailer cleavage

Inferred from electronic annotation. Source: InterPro

   Molecular_function3'-tRNA processing endoribonuclease activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 319319Ribonuclease Z HAMAP-Rule MF_01818
PRO_0000155887

Sites

Active site661Proton acceptor Potential
Metal binding621Zinc 1; catalytic By similarity
Metal binding641Zinc 1; catalytic By similarity
Metal binding661Zinc 2; catalytic By similarity
Metal binding671Zinc 2; catalytic By similarity
Metal binding1391Zinc 1; catalytic By similarity
Metal binding2091Zinc 1; catalytic By similarity
Metal binding2091Zinc 2; catalytic By similarity
Metal binding2681Zinc 2; catalytic By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88FQ4 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: DAC1D0EC0F14C87A

FASTA31934,917
        10         20         30         40         50         60 
MDLLFLGTSA GVPTKARNVS ATAVIEASGS HWYLVDCGEG TQHRLLHTPL SIRDLRAIFI 

        70         80         90        100        110        120 
THVHGDHCFG LPGLLASAGM SGRTQPLEVI LPAVLHDWVR QGLVASDTFL PFELRLLPVE 

       130        140        150        160        170        180 
ELIAWRSETL QVTTVQLSHR VPSVGFVFTE INPEPRLDIQ RLDAEGITRG PLWGELAKGL 

       190        200        210        220        230        240 
TVTYDGQLLN GNDYLRPSRP PRRVIVCGDN DKPELLAAVA RGADVLVHEA TFTQAVVERT 

       250        260        270        280        290        300 
GGTFGHSTAA EVARFAEAAG VRNLVLTHFS ARYQNDPRRS PHIDNVRDEA LAHYSGQLTL 

       310 
AQDLQRYHLG RNGLLEASA

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE015451 Genomic DNA. Translation: AAN69625.1.
RefSeqNP_746161.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88FQ4.
ModBaseSearch...

Protein-protein interaction databases

STRING160488.PP_4033.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN69625; AAN69625; PP_4033.
GeneID1043330.
KEGGppu:PP_4033.
PATRIC19946684. VBIPsePut30601_4286.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1234.
HOGENOMHOG000272419.
KOK00784.
OMAEGTQHQF.
ProtClustDBCLSK909513.

Enzyme and pathway databases

BioCycPPUT160488:GIXO-4034-MONOMER.

Family and domain databases

HAMAPMF_01818. RNase_Z/BN.
InterProIPR001279. Beta-lactamas-like.
IPR013471. RNase_Z.
[Graphical view]
SMARTSM00849. Lactamase_B. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRNZ_PSEPK
AccessionPrimary (citable) accession number: Q88FQ4
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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