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Q88FN1 (GLGB_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
1,4-alpha-glucan branching enzyme GlgB

EC=2.4.1.18
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE
Gene names
Name:glgB
Ordered Locus Names:PP_4058
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length736 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP-Rule MF_00685

Catalytic activity

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP-Rule MF_00685

Pathway

Glycan biosynthesis; glycogen biosynthesis. HAMAP-Rule MF_00685

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00685

Sequence similarities

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 7367361,4-alpha-glucan branching enzyme GlgB HAMAP-Rule MF_00685
PRO_0000188731

Sites

Active site4171Nucleophile By similarity
Active site4701Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88FN1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: C43AFBAEE689D94E

FASTA73682,877
        10         20         30         40         50         60 
MNVTTRENGG LRQRDLDALA RAEHADPFAV LGPHGDGAGG QVVRAFLPNA LKVRIQARDD 

        70         80         90        100        110        120 
GRVLAEMEQG SLPGLFSAHL DKAQPYQLHI VWAGGEQVTE DPYSFGPQLG DMDLHLFAEG 

       130        140        150        160        170        180 
NHRDLSGRFG AQPTQVDGID GVCFSVWAPN ARRVSVVGDF NNWDGRRHPM RLRHGAGVWE 

       190        200        210        220        230        240 
LFIPRLGVGE TYKFEVLGKD GILPLKADPL ARATELPPST ASKVAGELSH AWQDHDWMAQ 

       250        260        270        280        290        300 
RAQRHAYNAP LSIYELHPGS WRCELDEAGE IGRFYNWREL AERLVPYVQE LGFTHIELLP 

       310        320        330        340        350        360 
IMEHPFGGSW GYQPLSMFAP TSRYGSAEDF AAFIDACHQG GIGVLLDWVP AHFPTDEHGL 

       370        380        390        400        410        420 
ARFDGTALYE YDNPLEGYHQ DWNTLIYNLG RNEVRGFMMA SALHWLKHFH IDGLRVDAVA 

       430        440        450        460        470        480 
SMLYRDYSRK AGEWVPNRHG GRENLEAIDF IRHLNGVAAH EAPGALIIAE ESTAWPGVSQ 

       490        500        510        520        530        540 
PTQQGGLGFA YKWNMGWMHD TLHYIQNDPV HRTYHHNEMS FGLIYAYSEH FILPISHDEV 

       550        560        570        580        590        600 
VHGKHSLIDK MPGDRWQKFA NLRAYLTFMW AHPGKKLLFM GCEFGQWREW NHDAELDWYL 

       610        620        630        640        650        660 
LQYPEHQGVQ RLVGDLNRLY REEPALHEQD CQPQGFQWLI GDDAQNSVYA WLRWSSSGEP 

       670        680        690        700        710        720 
VLVVANFTPV PREGYRIGVP FGERWQELLN SDAELYAGSN VGNLGAVASD EVASHGQPLS 

       730 
LALNLPPLGV LIMKPA 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015451 Genomic DNA. Translation: AAN69648.1.
RefSeqNP_746184.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88FN1.
SMRQ88FN1. Positions 124-735.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160488.PP_4058.

Protein family/group databases

CAZyCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN69648; AAN69648; PP_4058.
GeneID1044393.
KEGGppu:PP_4058.
PATRIC19946740. VBIPsePut30601_4314.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0296.
HOGENOMHOG000283037.
KOK00700.
OMAGELSHAW.
OrthoDBEOG6JX7GT.

Enzyme and pathway databases

BioCycPPUT160488:GIXO-4137-MONOMER.
UniPathwayUPA00164.

Family and domain databases

Gene3D2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPMF_00685. GlgB.
InterProIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFPIRSF000463. GlgB. 1 hit.
SUPFAMSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 2 hits.
TIGRFAMsTIGR01515. branching_enzym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGLGB_PSEPK
AccessionPrimary (citable) accession number: Q88FN1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 15, 2003
Last sequence update: June 1, 2003
Last modified: June 11, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries