NADH-quinone oxidoreductase subunit G - Pseudomonas putida (strain KT2440)

Names and origin

Protein names

Recommended name:
    NADH-quinone oxidoreductase subunit G
    EC=1.6.99.5
Alternative name(s):
    NADH dehydrogenase I subunit G
    NDH-1 subunit G

Gene names

Name:	nuoG
Ordered Locus Names:	PP_4124

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Hide | Top

Protein attributes

Sequence length	904 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

Hide | Top

General annotation (Comments)

Function	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity.
Catalytic activity	NADH + quinone = NAD⁺ + quinol.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity. Binds 3 4Fe-4S clusters per subunit By similarity.
Subunit structure	Composed of 13 different subunits. Subunits nuoCD, E, F, and G constitute the peripheral sector of the complex.
Sequence similarities	Belongs to the complex I 75 kDa subunit family. Contains 1 2Fe-2S ferredoxin-type domain.

Hide | Top

Ontologies

Keywords
Ligand	2Fe-2S 4Fe-4S Iron Iron-sulfur Metal-binding NAD Ubiquinone
Molecular function	Oxidoreductase
PTM	Quinone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ATP synthesis coupled electron transport Inferred from electronic annotation. Source: InterPro
Cellular component	membrane Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW NADH dehydrogenase (ubiquinone) activity Inferred from electronic annotation. Source: InterPro electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW quinone binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 904

904

NADH-quinone oxidoreductase subunit G

PRO_0000118551

Regions

Domain

1 – 83

83

2Fe-2S ferredoxin-type

Sites

Metal binding

34

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

45

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

48

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

67

1

Iron-sulfur 1 (2Fe-2S) By similarity

Metal binding

99

1

Iron-sulfur 2 (4Fe-4S); via pros nitrogen By similarity

Metal binding

103

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

106

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

112

1

Iron-sulfur 2 (4Fe-4S) By similarity

Metal binding

151

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

154

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

157

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

201

1

Iron-sulfur 3 (4Fe-4S) By similarity

Metal binding

228

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

231

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

235

1

Iron-sulfur 4 (4Fe-4S) Potential

Metal binding

263

1

Iron-sulfur 4 (4Fe-4S) Potential

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

Q88FH2-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 6A33D03B3292B3F7
Show »

FASTA

904

97,582

        10         20         30         40         50         60 
MATIHVDGKA LEVNGADNLL QACLSLGLDI PYFCWHPALG SVGACRQCAV KQYTDENDTR 

        70         80         90        100        110        120 
GRIVMSCMTP ASDGTWISID DEESKAFRAS VVEWLMTNHP HDCPVCEEGG HCHLQDMTVM 

       130        140        150        160        170        180 
TGHNERRYRF TKRTHQNQDL GPFIAHEMNR CIACYRCVRY YKDYAGGTDL GVYGAHDNVY 

       190        200        210        220        230        240 
FGRVEDGVLE SEFSGNLTEV CPTGVFTDKT HSERYNRKWD MQFAPSICHG CSSGCNISPG 

       250        260        270        280        290        300 
ERYGELRRIE NRFNGSVNQY FLCDRGRFGY GYVNRKDRPR QPQLADGTKL GLDAALDKAA 

       310        320        330        340        350        360 
DLLRGRTIVG IGSPRASLES NYGLRELVGA EYFYSGMEAG ELARVRLALN VLNNSPLPVP 

       370        380        390        400        410        420 
TLRDIEDHDA VFVLGEDLTQ TAARVALAVR QATKGKAEAM AEAMKVQPWL DAAVKNIGQH 

       430        440        450        460        470        480 
ALYPLFIASL AETKLDDVAE ECVHAAPADL ARIGFAVAHA IDPSAPAVAG LDDEAQALAQ 

       490        500        510        520        530        540 
RIADALVAAK RPLVVAGTSL ADPALIEAAA NIAKALKLRE KNGSLSLVVP EANSLGLAML 

       550        560        570        580        590        600 
GGESVDAALD AVISGKADAI VVLENDLYAR VPAAKVDAAL AAAKVVIVAD HSKTATVDRA 

       610        620        630        640        650        660 
HLVLPAASFA EGDGTLVSQE GRAQRFFQVF DPQYLDSSIQ IHEGWRWMHA LRATLLNKPV 

       670        680        690        700        710        720 
DWTQLDHVTS ACAEAAPQLA GIVNAAPSAA FRIKGMKLAR EPLRYSGRTA MRANISVHEP 

       730        740        750        760        770        780 
RTPQDKDTAF AFSMEGYSGS AEPRQQVPFA WSPGWNSPQA WNKFQDEVGG HLRAGDPGVR 

       790        800        810        820        830        840 
LIESQGDRLN WFNAIPGAFN PARGIWTAVP FFHLFGSEES SSRAAPVQER IPAAYVALAK 

       850        860        870        880        890        900 
SEADRLGVND GALLSLNVAG VALRLPLRIN EELGAGLVAL PKGLAGIPPA IFGASVEGLQ 


EAAQ

« Hide

Hide | Top

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440."
Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O.

Fraser C.M.
Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Hide | Top

Cross-references

Sequence databases
	AE015451 Genomic DNA. Translation: AAN69707.1.
RefSeq	NP_746243.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1042269.
GenomeReviews	Gene locus PP_4124 in contig AE015451_GR.
KEGG	ppu:PP_4124.
NMPDR	fig\|160488.1.peg.4073.
TIGR	PP_4124.
Phylogenomic databases
HOGENOM	Q88FH2.
OMA	Q88FH2. EVGGHLR.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4124-MON.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001041. Ferredoxin. IPR006656. Mopterin_OxRdtase. IPR006963. Mopterin_OxRdtase_Fe4S4. IPR000283. NADH_UbQ_OxRdtase_75KDa_su_CS. IPR010228. NADH_UbQ_OxRdtase_Gsu. IPR019574. NADH_UbQ_OxRdtase_Gsu_4Fe4S-bd. [Graphical view]
Pfam	PF00111. Fer2. 1 hit. PF04879. Molybdop_Fe4S4. 1 hit. PF00384. Molybdopterin. 1 hit. PF10588. NADH-G_4Fe-4S_3. 1 hit. [Graphical view]
TIGRFAMs	TIGR01973. NuoG. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. False negative. PS51085. 2FE2S_FER_2. 1 hit. PS00641. COMPLEX1_75K_1. 1 hit. PS00642. COMPLEX1_75K_2. 1 hit. PS00643. COMPLEX1_75K_3. 1 hit. [Graphical view]
ProtoNet	Search...

Hide | Top

Entry information

Entry name

NUOG_PSEPK

Accession

Primary (citable) accession number: Q88FH2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents