Reviewed, UniProtKB/Swiss-Prot Q88EM0 (DADA1_PSEPK)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase 1 small subunit
    EC=1.4.99.1

Gene names

Name:	dadA1
Synonyms:	dadA-1
Ordered Locus Names:	PP_4434

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity.
Catalytic activity	A D-amino acid + H(2)O + acceptor = a 2-oxo acid + NH(3) + reduced acceptor.
Cofactor	FAD By similarity.
Subunit structure	Heterodimer of a small and a large subunit By similarity.
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 432	432	D-amino acid dehydrogenase 1 small subunit		PRO_0000166140
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q88EM0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4D1B8B220915C751	FASTA	432	47,155
	10 20 30 40 50 60 MRVLILGSGV VGTVSAYYLA REGFEVVVVD RQDGPAMETS FANAGQVSPG YASPWSAPGV 70 80 90 100 110 120 PLKAIRWMLQ QHAPLAIKPT SSLDQYRWMI QMLRNCNPAS YAVNKERMVR ISEYSRDCLD 130 140 150 160 170 180 ELRAETGISY EARQLGTTQL FRTQAQVDNA AKDIAILKQS GVPFELLDRD GIAKAEPALA 190 200 210 220 230 240 SVRDKLAGAL RLPNDQTGDC QMFTRKLAKM AEELGVEFRF GCNIDRLECS GDTISGVWID 250 260 270 280 290 300 GKLEVADQYV LALGSYSPHM LKPLGIQAPI YPLKGYSLTI PITDASMAPS STILDETYKV 310 320 330 340 350 360 AITRFDDRIR VGGMAEIAGF DLSLNARRRD TLELVVGDLY PKGGDLTKAT FWTGLRPATP 370 380 390 400 410 420 DGTPIVGKTK YRNLFLNTGH GTLGWTMSCG SGRLLADLMA GKKPKISAKG LDISRYSNQK 430 EAHNHGNPAT AL

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70011.1.
RefSeq	NP_746547.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1041878.
GenomeReviews	Gene locus PP_4434 in contig AE015451_GR.
KEGG	ppu:PP_4434.
NMPDR	fig|160488.1.peg.4377.
TIGR	PP_4434.
Phylogenomic databases
HOGENOM	Q88EM0.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4434-MON.
Family and domain databases
HAMAP	MF_01202. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR003042. Rng_hydrolase. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProDom	PD000139. FAD_pyr_redox. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

DADA1_PSEPK

Accession

Primary (citable) accession number: Q88EM0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 4, 2008
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)