Q88EI7 (ASTE_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Succinylglutamate desuccinylase
EC=3.5.1.96

Gene names

Name:	astE
Ordered Locus Names:	PP_4475

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Transforms N(2)-succinylglutamate into succinate and glutamate By similarity. HAMAP MF_00767
Catalytic activity	N-succinyl-L-glutamate + H2O = succinate + L-glutamate. HAMAP MF_00767
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00767
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 5/5. HAMAP MF_00767
Sequence similarities	Belongs to the AspA/AstE family. Succinylglutamate desuccinylase subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, acting on ester bonds Inferred from electronic annotation. Source: InterPro succinylglutamate desuccinylase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Succinylglutamate desuccinylase HAMAP MF_00767		PRO_0000174644
Sites
	Active site	215	1	Potential
	Metal binding	59	1	Zinc By similarity
	Metal binding	62	1	Zinc By similarity
	Metal binding	151	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88EI7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C68DDA68D3020FAA	FASTA	335	37,581
	10 20 30 40 50 60 MLALGKLLEL TLTDHEPAEK TQVTPKGARL RWLGEGALEV RPAESDDCGL DLLLSAGIHG 70 80 90 100 110 120 NETAPIELLE RLLHGVANGK IRPRARVLFL FGNPAAIRKG ERFIEQDINR LFNGRHELSS 130 140 150 160 170 180 GFEALRAAEL EQFARVFFSK PGRNRLHYDL HTAIRGSKIE QFALYPYKEG RKHSRRELAR 190 200 210 220 230 240 LAAAGMEAVL LQSKSSITFS AFTYEQLEAE AFTLELGKAR PFGQNEQVNL DKLEERLIRI 250 260 270 280 290 300 IEATEPEDES SLDGLQLFSV SREIIKHSDS FHLHLPADIE NFSELSKGYL LAEDLAEMRW 310 320 330 VVEEEGARII FPNPKVKNGL RAGILIVPDS GQRLG

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70050.1.
RefSeq	NP_746586.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88EI7.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88EI7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1044338.
GenomeReviews	Gene locus PP_4475 in contig AE015451_GR.
KEGG	ppu:PP_4475.
NMPDR	fig|160488.1.peg.4416.
PATRIC	19947648. VBIPsePut30601_4761.
TIGR	PP_4475.
Phylogenomic databases
eggNOG	COG2988.
HOGENOM	HBG298271.
OMA	YLHNDLN.
PhylomeDB	Q88EI7.
ProtClustDB	PRK05324.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4475-MONOMER.
Family and domain databases
HAMAP	MF_00767. Arg_catab_AstE. [Tree]
InterPro	IPR007036. Aste_AspA. IPR016681. SuccinylGlu_desuccinylase. [Graphical view]
KO	K05526.
Pfam	PF04952. AstE_AspA. 1 hit. [Graphical view]
PIRSF	PIRSF017020. AstE. 1 hit.
TIGRFAMs	TIGR03242. Arg_catab_astE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ASTE_PSEPK

Accession

Primary (citable) accession number: Q88EI7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program