Reviewed, UniProtKB/Swiss-Prot Q88EI4 (ASTD_PSEPK)

Names and origin

Protein names

Recommended name:
    N-succinylglutamate 5-semialdehyde dehydrogenase
    EC=1.2.1.71
Alternative name(s):
    Succinylglutamic semialdehyde dehydrogenase
      Short name=SGSD

Gene names

Name:	astD
Ordered Locus Names:	PP_4478

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NAD-dependent reduction of succinylglutamate semialdehyde into succinylglutamate By similarity.
Catalytic activity	N-succinyl-L-glutamate 5-semialdehyde + NAD(+) + H(2)O = N-succinyl-L-glutamate + NADH.
Pathway	Amino-acid degradation; L-arginine degradation via AST pathway; L-glutamate and succinate from L-arginine: step 4/5.
Sequence similarities	Belongs to the aldehyde dehydrogenase family. AstD subfamily.

Ontologies

Keywords
Biological process	Arginine metabolism
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine catabolic process to glutamate Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	succinylglutamate-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	N-succinylglutamate 5-semialdehyde dehydrogenase		PRO_0000262416
Regions
	Nucleotide binding	221 – 226	6	NAD By similarity
Sites
	Active site	244	1	By similarity
	Active site	278	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88EI4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E681006D4C5FCE8A	FASTA	487	51,330
	10 20 30 40 50 60 MTTHYIAGNW QAGQGETLQS LNPVTQAVIW QGQGADASQV DAAVQAARQA FPAWAQLSLE 70 80 90 100 110 120 ARIDVLEKFA AQLKVHAEAM AQCIGEETGK PLWESATEVT SMINKVAISV QSYRERTGEK 130 140 150 160 170 180 SGPLADATAV LRHKPHGVVA VFGPYNFPGH LPNGHIVPAL LAGNCVVFKP SELTPKVAEL 190 200 210 220 230 240 TVNCWIAAGL PAGVLNLVQG ARETGVALAA NPGIDGLFFT GSSRTGNLLH QQFAGRPDKI 250 260 270 280 290 300 LALEMGGNNP LVVDEVKDLD AAVYTIIQSA FISAGQRCTC ARRLLVPQGA WGDALIARLV 310 320 330 340 350 360 EVCKTITVGA FDEQPAPFMG SVISLQAARA LLAAQVELAA KGGVKLLEMT QPQADAALLT 370 380 390 400 410 420 PGIVDVTAVA DRPDEEFFGP LLQVIRYADF DAAIDEANNT QYGLAAGLLS DSRARYQYFW 430 440 450 460 470 480 LRSRAGIVNW NKQLTGAASS APFGGVGASG NHRASAYYAA DYCAYPVASL ETASLALPAT LTPGVTL

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70053.1.
RefSeq	NP_746589.1.
3D structure databases
HSSP	HSSP built from PDB template 1O9J based on UniProtKB Q28399.
ModBase	Search...
Genome annotation databases
GeneID	1043982.
GenomeReviews	Gene locus PP_4478 in contig AE015451_GR.
KEGG	ppu:PP_4478.
NMPDR	fig|160488.1.peg.4419.
TIGR	PP_4478.
Phylogenomic databases
HOGENOM	Q88EI4.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4478-MON.
Family and domain databases
HAMAP	MF_01174. [Tree]
InterPro	IPR016160. Ald_DHase_CS. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR017649. SuccinylGlu_semiald_DH_AstD. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASTD_PSEPK

Accession

Primary (citable) accession number: Q88EI4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)