Reviewed, UniProtKB/Swiss-Prot Q88EH6 (ACSA1_PSEPK)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase 1
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase 1
    Acyl-activating enzyme 1

Gene names

Name:	acsA1
Synonyms:	acsA
Ordered Locus Names:	PP_4487

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	653 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 653	653	Acetyl-coenzyme A synthetase 1 HAMAP MF_01123		PRO_0000208376
Sites
	Active site	517	1	By similarity
Amino acid modifications
	Modified residue	609	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88EH6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: CE626C86C922E8F4	FASTA	653	71,797
	10 20 30 40 50 60 MSAAPLYPVR PEVAATTLTD EATYKAMYQQ SVINPDGFWR EQAQRIDWIK PFTKVKQTSF 70 80 90 100 110 120 DDHHVDIKWF ADGTLNVSSN CLDRHLEERG DQLAIIWEGD DPSEHRNITY RELHEQVCKF 130 140 150 160 170 180 ANALRGQDVH RGDVVTIYMP MIPEAVVAML ACARIGAIHS VVFGGFSPEA LAGRIIDCKS 190 200 210 220 230 240 KVVITADEGV RGGRRTPLKA NVDLALTNPE TSSVQKIIVC KRTGGDIAWH QHRDIWYEDL 250 260 270 280 290 300 MKVASSHCAP KEMGAEEALF ILYTSGSTGK PKGVLHTTGG YLVYAALTHE RVFDYRPGEV 310 320 330 340 350 360 YWCTADVGWV TGHSYIVYGP LANGATTLLF EGVPNYPDIT RVSKIVDKHK VNILYTAPTA 370 380 390 400 410 420 IRAMMAEGQA AVEGADGSSL RLLGSVGEPI NPEAWNWYYK TVGKERCPIV DTWWQTETGG 430 440 450 460 470 480 ILISPLPGAT GLKPGSATRP FFGVVPALVD NLGNLIDGAA EGNLVILDSW PGQSRSLYGD 490 500 510 520 530 540 HDRFVDTYFK TFRGMYFTGD GARRDEDGYY WITGRVDDVL NVSGHRMGTA EIESAMVAHS 550 560 570 580 590 600 KVAEAAVVGV PHDIKGQGIY VYVTLNAGIE ASEQLRLELK NWVRKEIGPI ASPDVIQWAP 610 620 630 640 650 GLPKTRSGKI MRRILRKIAT GEYDALGDIS TLADPGVVQH LIDTHKAMNL ASA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70062.1.
RefSeq	NP_746598.1.
3D structure databases
HSSP	HSSP built from PDB template 1PG4 based on UniProtKB Q8ZKF6.
ModBase	Search...
Genome annotation databases
GeneID	1042436.
GenomeReviews	Gene locus PP_4487 in contig AE015451_GR.
KEGG	ppu:PP_4487.
NMPDR	fig|160488.1.peg.4428.
TIGR	PP_4487.
Phylogenomic databases
HOGENOM	Q88EH6.
OMA	Q88EH6. ETASEHC.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4487-MON.
Family and domain databases
HAMAP	MF_01123. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA1_PSEPK

Accession

Primary (citable) accession number: Q88EH6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)