UniProtKB - Q88EA7 (DEF2_PSEPK)
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Protein
Peptide deformylase 2
Gene
def2
Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Functioni
Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation
Catalytic activityi
Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation
Cofactori
Fe2+UniRule annotationNote: Binds 1 Fe2+ ion.UniRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 101 | IronUniRule annotation | 1 | |
Metal bindingi | 143 | IronUniRule annotation | 1 | |
Active sitei | 144 | UniRule annotation | 1 | |
Metal bindingi | 147 | IronUniRule annotation | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- peptide deformylase activity Source: UniProtKB-EC
GO - Biological processi
- translation Source: UniProtKB-KW
Keywordsi
Molecular function | Hydrolase |
Biological process | Protein biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | PPUT160488:G1G01-4867-MONOMER. |
Names & Taxonomyi
Protein namesi | Recommended name: Peptide deformylase 2UniRule annotation (EC:3.5.1.88UniRule annotation)Short name: PDF 2UniRule annotation Alternative name(s): Polypeptide deformylase 2UniRule annotation |
Gene namesi | Name:def2UniRule annotation Ordered Locus Names:PP_4559 |
Organismi | Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440) |
Taxonomic identifieri | 160488 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000082821 | 1 – 178 | Peptide deformylase 2Add BLAST | 178 |
Structurei
3D structure databases
ProteinModelPortali | Q88EA7. |
SMRi | Q88EA7. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the polypeptide deformylase family.UniRule annotation
Phylogenomic databases
eggNOGi | ENOG4108RBJ. Bacteria. COG0242. LUCA. |
HOGENOMi | HOG000243508. |
KOi | K01462. |
OMAi | DGFHARV. |
Family and domain databases
CDDi | cd00487. Pep_deformylase. 1 hit. |
Gene3Di | 3.90.45.10. 1 hit. |
HAMAPi | MF_00163. Pep_deformylase. 1 hit. |
InterProi | View protein in InterPro IPR023635. Peptide_deformylase. IPR036821. Peptide_deformylase_sf. |
PANTHERi | PTHR10458. PTHR10458. 1 hit. |
Pfami | View protein in Pfam PF01327. Pep_deformylase. 1 hit. |
PIRSFi | PIRSF004749. Pep_def. 1 hit. |
PRINTSi | PR01576. PDEFORMYLASE. |
SUPFAMi | SSF56420. SSF56420. 1 hit. |
TIGRFAMsi | TIGR00079. pept_deformyl. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q88EA7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRDILKMGD ERLLRIAPPV PEHMLGSAEL QQLIDDMFET MRHVGGVGLA
60 70 80 90 100
APQVGIDLQL VIFGFERSER YPDAEAVPQT ILLNPVITPT SSEVEDGWEG
110 120 130 140 150
CLSVPGLRGV VPRFKHICYQ GIDPQGSPIN RFADGFHARV VQHECDHLIG
160 170
RLYPSRIQDF AKFGYTEVLF PGLEVSED
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE015451 Genomic DNA. Translation: AAN70132.1. |
RefSeqi | NP_746668.1. NC_002947.4. WP_010955231.1. NC_002947.4. |
Genome annotation databases
EnsemblBacteriai | AAN70132; AAN70132; PP_4559. |
GeneIDi | 1046247. |
KEGGi | ppu:PP_4559. |
PATRICi | fig|160488.4.peg.4862. |
Similar proteinsi
Entry informationi
Entry namei | DEF2_PSEPK | |
Accessioni | Q88EA7Primary (citable) accession number: Q88EA7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 27, 2003 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 28, 2018 | |
This is version 90 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |