Reviewed, UniProtKB/Swiss-Prot Q88EA1 (ACEK_PSEPK)

Names and origin

Protein names

Recommended name:
    Isocitrate dehydrogenase kinase/phosphatase
      Short name=IDH kinase/phosphatase
      Short name=IDHK/P
    EC=2.7.11.5
    EC=3.1.3.-

Gene names

Name:	aceK
Ordered Locus Names:	PP_4565

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	571 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme which can phosphorylate or dephosphorylate isocitrate dehydrogenase (IDH) on a specific serine residue. This is a regulatory mechanism which enables bacteria to bypass the Krebs cycle via the glyoxylate shunt in response to the source of carbon. When bacteria are grown on glucose, IDH is fully active and unphosphorylated, but when grown on acetate or ethanol, the activity of IDH declines drastically concomitant with its phosphorylation By similarity.
Catalytic activity	ATP + [isocitrate dehydrogenase (NADP+)] = ADP + [isocitrate dehydrogenase (NADP+)] phosphate. HAMAP MF_00747
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the aceK family.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Ligand	ATP-binding NADP Nucleotide-binding
Molecular function	Hydrolase Kinase Protein phosphatase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP [isocitrate dehydrogenase (NADP+)] kinase activity Inferred from electronic annotation. Source: HAMAP phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 571	571	Isocitrate dehydrogenase kinase/phosphatase HAMAP MF_00747		PRO_0000057904
Regions
	Nucleotide binding	318 – 324	7	ATP By similarity
Sites
	Active site	374	1	By similarity
	Binding site	339	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88EA1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0A94EE44E65C1298	FASTA	571	65,954
	10 20 30 40 50 60 MSQPWPAVEI ARMILAGFDD YRDHFQRITL GARQRFEQAR WQDIQQAAAA RINLYEEKVA 70 80 90 100 110 120 EVNGWLRQAF AAEVLLDVEQ WPLVKNAYIH LIDPRLDDEL AETWYNSLFC SLFSHDLISD 130 140 150 160 170 180 GCMFIHTTRP SMRGRERAAQ TRTYRLDGSL RNLLRAVFAD YPFDMPYGDL EGDLARLEEQ 190 200 210 220 230 240 LRECLPDWVC KDPALAVELF VPVLYRNKGA YLVGRLYNSD EQWPLVIPLL HREGHGIEAD 250 260 270 280 290 300 ALITDEAEVS IIFSFTRSYF MADVPVPAEF VNFLKRILPG KHIAELYTSI GFYKQGKSEF 310 320 330 340 350 360 YRALINHLAS SDDRFVMAPG VRGMVMSVFT LPGFNTVFKI IKDRFSPSKT VDRATVIDKY 370 380 390 400 410 420 RLVKSVDRVG RMADTQEFAD FRFPRSKFEP DCLAELLEVA PSTVALEGDT VLIRHCWTER 430 440 450 460 470 480 RMTPLNLYLE QATEGQVLEA LEDYGLAIKQ LAAANIFPGD MLLKNFGVTR HGRVVFYDYD 490 500 510 520 530 540 EISFLTEVNF RHIPPPRYPE DEMSGEPWYS IGPHDVFPEE FPPFLFADMG QRRLFSRLHG 550 560 570 ELYDADYWKG LQAAIREGKV IDVFPYRRKA R

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70138.1.
RefSeq	NP_746674.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q88EA1.
Genome annotation databases
GeneID	1042619.
GenomeReviews	Gene locus PP_4565 in contig AE015451_GR.
KEGG	ppu:PP_4565.
NMPDR	fig|160488.1.peg.4504.
TIGR	PP_4565.
Phylogenomic databases
HOGENOM	Q88EA1.
OMA	FYRDFLH.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4565-MON.
Family and domain databases
HAMAP	MF_00747. [Tree]
InterPro	IPR010452. Isocitrate_DH_AceK. [Graphical view]
Pfam	PF06315. AceK. 1 hit. [Graphical view]
PIRSF	PIRSF000719. AceK. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ACEK_PSEPK

Accession

Primary (citable) accession number: Q88EA1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)