Q88E47 (HGD_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Homogentisate 1,2-dioxygenase
EC=1.13.11.5

Alternative name(s):
Homogentisate oxygenase
Homogentisic acid oxidase
Homogentisicase

Gene names

Name:	hmgA
Ordered Locus Names:	PP_4621

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas › Pseudomonas putida group › Pseudomonas putida

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Homogentisate + O2 = 4-maleylacetoacetate. HAMAP-Rule MF_00334
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetate and fumarate from L-phenylalanine: step 4/6. HAMAP-Rule MF_00334
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homogentisate 1,2-dioxygenase HAMAP-Rule MF_00334		PRO_0000220250
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	337	1	Iron By similarity
	Metal binding	367	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88E47 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: 6D5F4C7F91BE4512	FASTA	433	48,011
	10 20 30 40 50 60 MNRDTSPDLH YLSGFGNEFA SEALPGALPV GQNSPQKAPY GLYAELLSGT AFTMARSELR 70 80 90 100 110 120 RTWLYRIRPS ALHPRFERLA RQPLGGPLGG INPNRLRWSP QPIPAEPTDF IEGWLPMAAN 130 140 150 160 170 180 AGAEKPAGVS IYIYRANRSM ERVFFNADGE LLLVPEQGRL RIATELGVME VEPLEIAVIP 190 200 210 220 230 240 RGMKFRVELL DGQARGYIAE NHGAPLRLPD LGPIGSNGLA NPRDFLTPVA HYEEAEGPVQ 250 260 270 280 290 300 LVQKFLGEHW ACELQHSPLD VVAWHGSNVP YKYDLRRFNT IGTVSFDHPD PSIFTVLTSP 310 320 330 340 350 360 TSVHGMANMD FVIFPPRWMV AENTFRPPWF HRNLMNEFMG LINGAYDAKA EGFLPGGASL 370 380 390 400 410 420 HGVMSAHGPD AETCEKAIAA DLAPHKIDNT MAFMFETSQV LRPSLQALEC PQLQADYDSC 430 WATLPSTFNP NRR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70194.1.
RefSeq	NP_746730.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88E47.
ModBase	Search...
Protein-protein interaction databases
STRING	160488.PP_4621.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN70194; AAN70194; PP_4621.
GeneID	1041590.
KEGG	ppu:PP_4621.
PATRIC	19947980. VBIPsePut30601_4927.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3508.
HOGENOM	HOG000139824.
KO	K00451.
OMA	AVTKWGL.
ProtClustDB	PRK05341.
Enzyme and pathway databases
BioCyc	PPUT160488:GIXO-4622-MONOMER.
UniPathway	UPA00139; UER00339.
Family and domain databases
Gene3D	2.60.120.10. 2 hits.
HAMAP	MF_00334. Homogentis_dioxygen.
InterPro	IPR005708. Homogentis_dOase. IPR022950. Homogentis_dOase_bac. IPR014710. RmlC-like_jellyroll. IPR011051. RmlC_Cupin. [Graphical view]
PANTHER	PTHR11056. PTHR11056. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
SUPFAM	SSF51182. RmlC_like_cupin. 1 hit.
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEPK

Accession

Primary (citable) accession number: Q88E47

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program