Reviewed, UniProtKB/Swiss-Prot Q88E47 (HGD_PSEPK)

Names and origin

Protein names

Recommended name:
    Homogentisate 1,2-dioxygenase
    EC=1.13.11.5
Alternative name(s):
    Homogentisicase
    Homogentisate oxygenase
    Homogentisic acid oxidase

Gene names

Name:	hmgA
Ordered Locus Names:	PP_4621

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	433 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Homogentisate + O(2) = 4-maleylacetoacetate.
Cofactor	Iron By similarity.
Pathway	Amino-acid degradation; L-phenylalanine degradation; acetoacetic acid and fumarate from L-phenylalanine: step 4/6.
Sequence similarities	Belongs to the homogentisate dioxygenase family.

Ontologies

Keywords
Biological process	Phenylalanine catabolism Tyrosine catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-phenylalanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW tyrosine catabolic process Inferred from electronic annotation. Source: HAMAP
Molecular function	homogentisate 1,2-dioxygenase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 433	433	Homogentisate 1,2-dioxygenase		PRO_0000220250
Sites
	Metal binding	331	1	Iron By similarity
	Metal binding	337	1	Iron By similarity
	Metal binding	367	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88E47-1 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: 6D5F4C7F91BE4512	FASTA	433	48,011
	10 20 30 40 50 60 MNRDTSPDLH YLSGFGNEFA SEALPGALPV GQNSPQKAPY GLYAELLSGT AFTMARSELR 70 80 90 100 110 120 RTWLYRIRPS ALHPRFERLA RQPLGGPLGG INPNRLRWSP QPIPAEPTDF IEGWLPMAAN 130 140 150 160 170 180 AGAEKPAGVS IYIYRANRSM ERVFFNADGE LLLVPEQGRL RIATELGVME VEPLEIAVIP 190 200 210 220 230 240 RGMKFRVELL DGQARGYIAE NHGAPLRLPD LGPIGSNGLA NPRDFLTPVA HYEEAEGPVQ 250 260 270 280 290 300 LVQKFLGEHW ACELQHSPLD VVAWHGSNVP YKYDLRRFNT IGTVSFDHPD PSIFTVLTSP 310 320 330 340 350 360 TSVHGMANMD FVIFPPRWMV AENTFRPPWF HRNLMNEFMG LINGAYDAKA EGFLPGGASL 370 380 390 400 410 420 HGVMSAHGPD AETCEKAIAA DLAPHKIDNT MAFMFETSQV LRPSLQALEC PQLQADYDSC 430 WATLPSTFNP NRR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70194.1.
RefSeq	NP_746730.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1041590.
GenomeReviews	Gene locus PP_4621 in contig AE015451_GR.
KEGG	ppu:PP_4621.
NMPDR	fig|160488.1.peg.4560.
TIGR	PP_4621.
Phylogenomic databases
HOGENOM	Q88E47.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4621-MON.
Family and domain databases
HAMAP	MF_00334. [Tree]
InterPro	IPR005708. Homogentis_dOase. [Graphical view]
PANTHER	PTHR11056. Homogentis_dOase. 1 hit.
Pfam	PF04209. HgmA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01015. hmgA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HGD_PSEPK

Accession

Primary (citable) accession number: Q88E47

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	November 4, 2008
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)