Reviewed, UniProtKB/Swiss-Prot Q88E20 (RLMG_PSEPK)

Names and origin

Protein names

Recommended name:
    Ribosomal RNA large subunit methyltransferase G
    EC=2.1.1.52
Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase rlmG
    23S rRNA m2G1835 methyltransferase

Gene names

Name:	rlmG
Ordered Locus Names:	PP_4648

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates the guanosine in position 1835 (m2G1835) of 23S rRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine + rRNA containing N(2)-methylguanine. HAMAP MF_01859
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmG family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA methylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	rRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	Ribosomal RNA large subunit methyltransferase G HAMAP MF_01859		PRO_0000366481

Sequences

	Sequence		Length	Mass (Da)
	Q88E20-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: FF8BD908C3246D53	FASTA	374	40,473
	10 20 30 40 50 60 MPLLTTPYAE LDLIRQPEQA NDPLQAFDAA DEYLLAQLHD QAPDANCRVL VLNDSFGALA 70 80 90 100 110 120 ASLAGQLQVV SSGDSHLGHL ALEKNLARNG LPFDSVPFVP ASEHWQGPFD RVLVRVPKTL 130 140 150 160 170 180 ALLEEQLIRL QGQLAPGAQV IAGAMIKHLP RAAGDLMEKY IGPVQASLAL KKARLLTATV 190 200 210 220 230 240 AERPLAKSPY PSCYRLDAPA LDLVNHANVF CREGLDIGTR AFLPHLPRNL GRARVADLGC 250 260 270 280 290 300 GNGVLAIASA LANPEAEYTL VDESYMAVQS AQENWLAALG ERPATFLAAD GLAGLEKQSL 310 320 330 340 350 360 DVVLCNPPFH QQQVVGDFLA WRMFQQAREA LVVGGALYIV GNRHLGYHSK LARLFRGVEQ 370 VAATPKFVIL KARK

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70221.1.
RefSeq	NP_746757.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q88E20.
Genome annotation databases
GeneID	1046256.
GenomeReviews	Gene locus PP_4648 in contig AE015451_GR.
KEGG	ppu:PP_4648.
NMPDR	fig|160488.1.peg.4587.
TIGR	PP_4648.
Phylogenomic databases
HOGENOM	Q88E20.
OMA	NRHLGYH.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4648-MON.
Family and domain databases
HAMAP	MF_01859. [Tree]
InterPro	IPR017237. rRNA_m2G-MeTrfase_RsmD. IPR007848. Small_mtfrase. [Graphical view]
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037565. RRNA_m2G_Mtase_RsmD_prd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMG_PSEPK

Accession

Primary (citable) accession number: Q88E20

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)