Reviewed, UniProtKB/Swiss-Prot Q88DW6 (ACSA2_PSEPK)

Names and origin

Protein names

Recommended name:
    Acetyl-coenzyme A synthetase 2
    EC=6.2.1.1
Alternative name(s):
    Acetate--CoA ligase 2
    Acyl-activating enzyme 2

Gene names

Name:	acsA2
Synonyms:	acsB
Ordered Locus Names:	PP_4702

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	644 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity. HAMAP MF_01123
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	AMP binding Inferred from electronic annotation. Source: InterPro ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 644	644	Acetyl-coenzyme A synthetase 2 HAMAP MF_01123		PRO_0000208377
Sites
	Active site	513	1	By similarity
Amino acid modifications
	Modified residue	605	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88DW6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 82D98FD672B86A9A	FASTA	644	71,251
	10 20 30 40 50 60 MFDIRKYPQA LAVSQSAALT PEDYRRLYRQ SVEDPDTFWA EQAKRLDWIK PWSSVQQCDL 70 80 90 100 110 120 HTGKARWFDG AQLNVSYNCI DRHLAQRGEQ TALLWEGDDP KDSKAITYRE LHRQVCRLAN 130 140 150 160 170 180 AMKARGVKKG DRVSIYMPMI PEAAFAMLAC TRIGAIHSVV FGGFSPDALR DRILDADCRT 190 200 210 220 230 240 VITADEGVRG GKRIPLKQNV DKALASCPAV SSVLVVRRTG GDVAWTEGRD LWYHEATKDA 250 260 270 280 290 300 GDDCPPEPME AEDPLFILYT SGSTGKPKGV LHTTGGYLLQ ATMTFKVVFD YRDGEVFWCT 310 320 330 340 350 360 ADVGWVTGHS YIVYGPLANG AISLMFEGVP NYPDTSRFWQ VVDKHQVNIF YTAPTALRAL 370 380 390 400 410 420 MREGSAPLQS TSRKSLRLLG SVGEPINPEA WEWYFEEVGQ KRCPIVDTWW QTETGGIMLT 430 440 450 460 470 480 PLPGTQSLKP GCATQPMFGV QPVLLDEKGK LIEGPGAGVL AIKASWPGQI RSVYGDHQRM 490 500 510 520 530 540 VDTYFKPLPG YYFTGDGARR DADGDYWITG RIDDVINVSG HRIGTAEVES ALVLHDSVAE 550 560 570 580 590 600 AAVVGYPHDL KGQGVYAFVT TMNGVTPDDT LKAELLALVS KEIGSFAKPE LIQWAPALPK 610 620 630 640 TRSGKIMRRI LRKIACNELE NLGDTSTLAD PSVVQGLIDK RLNQ

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70275.1.
RefSeq	NP_746811.1.
3D structure databases
SMR	Q88DW6. Positions 13-641.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88DW6.
Genome annotation databases
GeneID	1041843.
GenomeReviews	Gene locus PP_4702 in contig AE015451_GR.
KEGG	ppu:PP_4702.
NMPDR	fig|160488.1.peg.4641.
TIGR	PP_4702.
Phylogenomic databases
eggNOG	COG0365.
HOGENOM	HBG547964.
OMA	HQRMVDT.
PhylomeDB	Q88DW6.
Family and domain databases
HAMAP	MF_01123. Ac_CoA_synth. [Tree]
InterPro	IPR011904. Ac_CoA_lig_AcsA. IPR020845. AMP-binding_CS. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA2_PSEPK

Accession

Primary (citable) accession number: Q88DW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)