Reviewed, UniProtKB/Swiss-Prot Q88DT3 (LLDD_PSEPK)

Names and origin

Protein names

Recommended name:
    L-lactate dehydrogenase [cytochrome]
    EC=1.1.2.3

Gene names

Name:	lldD
Ordered Locus Names:	PP_4736

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	381 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c + 2 H+. HAMAP MF_01559
Cofactor	FMN By similarity. HAMAP MF_01559
Subunit structure	Homotetramer By similarity. HAMAP MF_01559
Sequence similarities	Belongs to the FMN-dependent alpha-hydroxy acid dehydrogenase family. Contains 1 FMN hydroxy acid dehydrogenase domain.

Ontologies

Keywords
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lactate metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FMN binding Inferred from electronic annotation. Source: InterPro L-lactate dehydrogenase (cytochrome) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 381	381	L-lactate dehydrogenase [cytochrome] HAMAP MF_01559		PRO_0000206342
Regions
	Domain	1 – 380	380	FMN hydroxy acid dehydrogenase
	Nucleotide binding	306 – 330	25	FMN By similarity
Sites
	Active site	275	1	Proton acceptor By similarity
	Binding site	24	1	Substrate Potential
	Binding site	106	1	FMN By similarity
	Binding site	127	1	FMN By similarity
	Binding site	129	1	Substrate By similarity
	Binding site	155	1	FMN By similarity
	Binding site	164	1	Substrate By similarity
	Binding site	251	1	FMN By similarity
	Binding site	278	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q88DT3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4EE62A72CB973A68	FASTA	381	41,392
	10 20 30 40 50 60 MIISASTDYR AAAQRKLPPF LFHYADGGAY AEHTLRHNVS DLAGIALRQR VLNNMSELSL 70 80 90 100 110 120 ETKLFDETLS MPVALAPVGL TGMYARRGEV QAARAAAAHG IPFTMSTVSV CPIEEVAPAI 130 140 150 160 170 180 NRPMWFQLYV LKDRGFMRNA LERAKAAGVK TLVFTVDMPV PGARYRDAHS GMSGKNGPLR 190 200 210 220 230 240 RVLQAMTHPE WAWDVGVMGR PHDLGNISKY RGNPTGLADY IGWLGNNFDP SISWKDLEWI 250 260 270 280 290 300 REFWDGPMII KGILDADDAR DAVKFGADGI VVSNHGGRQL DGVLSSARAL PAIADAVKGD 310 320 330 340 350 360 LKILADSGIR SGLDVVRMIA LGADTVLIGR AFLYALAVHG QAGVKNLLEL FEKEMRVAMV 370 380 LTGAKSISEI TRDSLVRELG A

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70308.1.
RefSeq	NP_746844.1.
3D structure databases
SMR	Q88DT3. Positions 1-378.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88DT3.
Genome annotation databases
GeneID	1043044.
GenomeReviews	Gene locus PP_4736 in contig AE015451_GR.
KEGG	ppu:PP_4736.
NMPDR	fig|160488.1.peg.4674.
TIGR	PP_4736.
Phylogenomic databases
eggNOG	COG1304.
HOGENOM	HBG517781.
OMA	MSTLATC.
PhylomeDB	Q88DT3.
Family and domain databases
HAMAP	MF_01559. L-lact_dehydr. [Tree]
InterPro	IPR012133. a-Hydoxy_acid_DH_FMN. IPR013785. Aldolase_TIM. IPR000262. FMN-dep_DH. IPR008259. FMN_hydac_DH_AS. IPR020920. L-lactate_DHase_cyt. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01070. FMN_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000138. Al-hdrx_acd_dh. 1 hit.
PROSITE	PS00557. FMN_HYDROXY_ACID_DH_1. 1 hit. PS51349. FMN_HYDROXY_ACID_DH_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LLDD_PSEPK

Accession

Primary (citable) accession number: Q88DT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 10, 2006
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)