Reviewed, UniProtKB/Swiss-Prot Q88D35 (GSHB_PSEPK)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	PP_4993

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	317 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 317	317	Glutathione synthetase HAMAP MF_00162		PRO_0000197477
Regions
	Domain	125 – 311	187	ATP-grasp
	Nucleotide binding	152 – 208	57	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88D35-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 9A731692A21C6E4F	FASTA	317	35,255
	10 20 30 40 50 60 MSVRLGIVMD PIASISYKKD SSLAMLLAAQ ARGWSLFYME QQDLYQGEGK ARARMRPLKV 70 80 90 100 110 120 FADPARWFEL GEEQDSPLAE LDVILMRKDP PFDMEFVYST YLLEQAENDG VLVVNRPQSL 130 140 150 160 170 180 RDCNEKMFAT LFPQCTTPTL VSRRPDIIRE FTAKHADVIL KPLDGMGGTS IFRHRAGDPN 190 200 210 220 230 240 LSVILETLTA LGTQQIMAQA YLPAIKDGDK RILMIDGEPV DYCLARIPAS GETRGNLAAG 250 260 270 280 290 300 GRGEARPLTE RDRWIAAQVG PTLREKGLLF VGLDVIGDYL TEINVTSPTC IREIDAAYNT 310 DIGGKLMDAI DRKLKAR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70559.1.
RefSeq	NP_747095.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSH based on UniProtKB P04425.
ModBase	Search...
Genome annotation databases
GeneID	1042200.
GenomeReviews	Gene locus PP_4993 in contig AE015451_GR.
KEGG	ppu:PP_4993.
NMPDR	fig|160488.1.peg.4925.
TIGR	PP_4993.
Phylogenomic databases
HOGENOM	Q88D35.
OMA	Q88D35. VRNAPEK.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_4993-MON.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_PSEPK

Accession

Primary (citable) accession number: Q88D35

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)