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Reviewed, UniProtKB/Swiss-Prot Q88D35 (GSHB_PSEPK)

Last modified June 16, 2009. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase
Gene names
Name: gshB
Ordered Locus Names: PP_4993
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length317 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 317317Glutathione synthetase HAMAP MF_00162
PRO_0000197477

Regions

Domain125 – 311187ATP-grasp
Nucleotide binding152 – 20857ATP By similarity

Sites

Metal binding2821Magnesium or manganese By similarity
Metal binding2841Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88D35-1 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 9A731692A21C6E4F

FASTA31735,255
        10         20         30         40         50         60 
MSVRLGIVMD PIASISYKKD SSLAMLLAAQ ARGWSLFYME QQDLYQGEGK ARARMRPLKV 

        70         80         90        100        110        120 
FADPARWFEL GEEQDSPLAE LDVILMRKDP PFDMEFVYST YLLEQAENDG VLVVNRPQSL 

       130        140        150        160        170        180 
RDCNEKMFAT LFPQCTTPTL VSRRPDIIRE FTAKHADVIL KPLDGMGGTS IFRHRAGDPN 

       190        200        210        220        230        240 
LSVILETLTA LGTQQIMAQA YLPAIKDGDK RILMIDGEPV DYCLARIPAS GETRGNLAAG 

       250        260        270        280        290        300 
GRGEARPLTE RDRWIAAQVG PTLREKGLLF VGLDVIGDYL TEINVTSPTC IREIDAAYNT 

       310 
DIGGKLMDAI DRKLKAR 

« Hide

Cross-references

Sequence databases

AE015451 Genomic DNA. Translation: AAN70559.1.
RefSeqNP_747095.1.

3D structure databases

HSSPHSSP built from PDB template 1GSH based on UniProtKB P04425.
ModBaseSearch...

Genome annotation databases

GeneID1042200.
GenomeReviewsGene locus PP_4993 in contig AE015451_GR.
KEGGppu:PP_4993.
NMPDRfig|160488.1.peg.4925.
TIGRPP_4993.

Phylogenomic databases

HOGENOMQ88D35.
OMAQ88D35. VRNAPEK.

Enzyme and pathway databases

BioCycPPUT160488:PP_4993-MON.

Family and domain databases

HAMAPMF_00162.
[Tree]
InterProIPR011761. ATP-grasp.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP_bd.
IPR004215. GSHS_N.
IPR013817. Pre-ATP_grasp.
[Graphical view]
Gene3DG3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_PSEPK
AccessionPrimary (citable) accession number: Q88D35
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents