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Protein

Urocanate hydratase

Gene

hutU

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate = urocanate + H2O.UniRule annotation

Cofactori

NAD+UniRule annotationNote: Binds 1 NAD+ per subunit.UniRule annotation

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 2 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei411UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Names & Taxonomyi

Protein namesi
Recommended name:
Urocanate hydrataseUniRule annotation (EC:4.2.1.49UniRule annotation)
Short name:
UrocanaseUniRule annotation
Alternative name(s):
Imidazolonepropionate hydrolaseUniRule annotation
Gene namesi
Name:hutUUniRule annotation
Ordered Locus Names:PP_5033
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002073451 – 557Urocanate hydrataseAdd BLAST557

Interactioni

Protein-protein interaction databases

STRINGi160488.PP_5033.

Structurei

3D structure databases

ProteinModelPortaliQ88CZ6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the urocanase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.
HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q88CZ6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDNNKYRDV EIRAPRGNKL TAKSWLTEAP LRMLMNNLDP QVAENPKELV
60 70 80 90 100
VYGGIGRAAR NWACYDKIVE TLTRLEDDET LLVQSGKPVG VFKTHSNAPR
110 120 130 140 150
VLIANSNLVP HWANWEHFNE LDAKGLAMYG QMTAGSWIYI GSQGIVQGTY
160 170 180 190 200
ETFVEAGRQH YGGSLKGKWV LTAGLGGMGG AQPLAATLAG ACSLNIECQQ
210 220 230 240 250
SRIDFRLETR YVDEQATDLD DALARIAKYT AEGKAISIAL HGNAAEILPE
260 270 280 290 300
LVKRGVRPDM VTDQTSAHDP LNGYLPAGWT WEQYRDRAQT EPAAVVKAAK
310 320 330 340 350
QSMAVHVQAM LDFQKQGIPT FDYGNNIRQM AKEEGVANAF DFPGFVPAYI
360 370 380 390 400
RPLFCRGVGP FRWAALSGEA EDIYKTDAKV KELIPDDAHL HRWLDMARER
410 420 430 440 450
ISFQGLPARI CWVGLGLRAK LGLAFNEMVR SGELSAPVVI GRDHLDSGSV
460 470 480 490 500
SSPNRETEAM RDGSDAVSDW PLLNALLNTA GGATWVSLHH GGGVGMGFSQ
510 520 530 540 550
HSGMVIVCDG TDEAAERIAR VLTNDPGTGV MRHADAGYDI AIDCAKEQGL

DLPMITG
Length:557
Mass (Da):60,718
Last modified:June 1, 2003 - v1
Checksum:i7605B25E4C2CBC31
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN70598.1.
RefSeqiNP_747134.1. NC_002947.4.
WP_004577162.1. NC_002947.4.

Genome annotation databases

GeneIDi1042837.
KEGGippu:PP_5033.
PATRICi19948890. VBIPsePut30601_5374.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA. Translation: AAN70598.1.
RefSeqiNP_747134.1. NC_002947.4.
WP_004577162.1. NC_002947.4.

3D structure databases

ProteinModelPortaliQ88CZ6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160488.PP_5033.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1042837.
KEGGippu:PP_5033.
PATRICi19948890. VBIPsePut30601_5374.

Phylogenomic databases

eggNOGiENOG4105CGP. Bacteria.
COG2987. LUCA.
HOGENOMiHOG000237606.
KOiK01712.
OMAiVSFHHGG.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00550.

Family and domain databases

HAMAPiMF_00577. HutU. 1 hit.
InterProiIPR023637. Urocanase.
IPR023636. Urocanase_CS.
[Graphical view]
PfamiPF01175. Urocanase. 1 hit.
[Graphical view]
PIRSFiPIRSF001423. Urocanate_hydrat. 1 hit.
SUPFAMiSSF111326. SSF111326. 1 hit.
TIGRFAMsiTIGR01228. hutU. 1 hit.
PROSITEiPS01233. UROCANASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHUTU_PSEPK
AccessioniPrimary (citable) accession number: Q88CZ6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: June 1, 2003
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.