Reviewed, UniProtKB/Swiss-Prot Q88CW6 (BETA_PSEPK)

Names and origin

Protein names

Recommended name:
    Choline dehydrogenase
      Short name=CHD
      Short name=CDH
    EC=1.1.99.1

Gene names

Name:	betA
Ordered Locus Names:	PP_5064

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Can catalyze the oxidation of choline to betaine aldehyde and betaine aldehyde to glycine betaine By similarity.
Catalytic activity	Choline + acceptor = betaine aldehyde + reduced acceptor.
Cofactor	FAD By similarity.
Pathway	Amine and polyamine biosynthesis; betaine biosynthesis via choline pathway; betaine aldehyde from choline (cytochrome c reductase route): step 1/1.
Sequence similarities	Belongs to the GMC oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cellular alcohol metabolic process Inferred from electronic annotation. Source: InterPro glycine betaine biosynthetic process from choline Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	FAD binding Inferred from electronic annotation. Source: InterPro choline dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Choline dehydrogenase		PRO_0000205591
Regions
	Nucleotide binding	6 – 35	30	FAD Probable
Sites
	Active site	475	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88CW6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 11C36EEBD7D6EF86	FASTA	565	62,545
	10 20 30 40 50 60 MSQEFDYIIV GAGSAGNTLA TRLTEDAGVT VLLLEAGGPD YRFDFRTQMP AALAFPLQGR 70 80 90 100 110 120 RYNWAYETDP EPYMDGRRME CGRGKGLGGS SLINGMCYIR GNAMDFDGWA ELPGLEDWTY 130 140 150 160 170 180 LDCLPYFRKA ETRDIGPNDY HGGEGPVSVT TPKAGNNPLF HAMVEAGVQA GYPRTEDLNG 190 200 210 220 230 240 YQQEGFGPMD RTVTKNGRRS STARGYLDQA KKRPNLTIVT HALTDRVLFD GKRAIGVTYL 250 260 270 280 290 300 VGDSEERVEA RARKEVIVSS GAIASPQLLQ RSGVGPRALL ESLDIPVVHD LPGVGENLQD 310 320 330 340 350 360 HLELYLQYAC TQPVSLYPSL LWWNQPAIGA EWMFNGTGIG ASNQFEAGGF IRTRPEFKWP 370 380 390 400 410 420 NIQYHFLPVA INYNGSNGVK EHGFQAHMGS MRSPARGRIQ VKSKDPRQHP SILFNYMSTE 430 440 450 460 470 480 QDWQEFRDGI RLTREIMAQP ALDPYRGREI SPGADVQTDE QLDKFIREHA ETAFHPSCSC 490 500 510 520 530 540 KMGTDDMAVV DGEGRVHGMK GLRVVDASIM PLIITGNLNA TTIMIAEKIS DKIRGRKPLP 550 560 RSTAKYYVAG DAPVKGKPMR EVKQG

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE015451 Genomic DNA. Translation: AAN70629.1.
RefSeq	NP_747165.1.
3D structure databases
HSSP	HSSP built from PDB template 1JU2 based on UniProtKB Q945K2.
ModBase	Search...
Genome annotation databases
GeneID	1043008.
GenomeReviews	Gene locus PP_5064 in contig AE015451_GR.
KEGG	ppu:PP_5064.
NMPDR	fig|160488.1.peg.4995.
TIGR	PP_5064.
Phylogenomic databases
HOGENOM	Q88CW6.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_5064-MON.
Family and domain databases
HAMAP	MF_00750. [Tree]
InterPro	IPR011533. Choline_dehydrogenase. IPR012132. GMC_OxRdtase. IPR000172. GMC_OxRdtase_N. IPR007867. GMC_OxRtase_C. [Graphical view]
Pfam	PF05199. GMC_oxred_C. 1 hit. PF00732. GMC_oxred_N. 1 hit. [Graphical view]
PIRSF	PIRSF000137. Alcohol_oxidase. 1 hit.
TIGRFAMs	TIGR01810. betA. 1 hit.
PROSITE	PS00623. GMC_OXRED_1. 1 hit. PS00624. GMC_OXRED_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

BETA_PSEPK

Accession

Primary (citable) accession number: Q88CW6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 25, 2008
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)