Q88CT0 (NTPA_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

PP_5100

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	198 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 198	198	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178214
Regions
	Region	11 – 16	6	Substrate binding By similarity
	Region	71 – 72	2	Substrate binding By similarity
Sites
	Metal binding	42	1	Magnesium or manganese By similarity
	Metal binding	71	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88CT0 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 0D51015ED7A8B87A	FASTA	198	21,303
	10 20 30 40 50 60 MMNFQQLVLA SHNAGKLKEL QAMLGHSVQL HSIGEFSQVE PEETGLSFVE NAILKARNAA 70 80 90 100 110 120 RISGLPALAD DSGLAVDFLG GAPGIYSARY ADGKGDAANN AKLLEALKDV PEAERSAQFV 130 140 150 160 170 180 CVLALVRHAD DPLPILCEGL WHGRILFEAS GDQGFGYDPL FWVPERNCSS ADLAPADKNQ 190 LSHRARAMAL LRKRLGLA

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70665.1.
RefSeq	NP_747201.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88CT0.
SMR	Q88CT0. Positions 4-195.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88CT0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1042681.
GenomeReviews	Gene locus PP_5100 in contig AE015451_GR.
KEGG	ppu:PP_5100.
NMPDR	fig|160488.1.peg.5031.
PATRIC	19949028. VBIPsePut30601_5443.
TIGR	PP_5100.
Phylogenomic databases
eggNOG	COG0127.
HOGENOM	HBG697237.
OMA	YSKRYDQ.
PhylomeDB	Q88CT0.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_5100-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NTPA_PSEPK

Accession

Primary (citable) accession number: Q88CT0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 24, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program