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Protein

Thymidylate synthase

Gene

thyA

Organism
Pseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reductive methylation of 2'-deoxyuridine-5'-monophosphate (dUMP) to 2'-deoxythymidine-5'-monophosphate (dTMP) while utilizing 5,10-methylenetetrahydrofolate (mTHF) as the methyl donor and reductant in the reaction, yielding dihydrofolate (DHF) as a by-product. This enzymatic reaction provides an intracellular de novo source of dTMP, an essential precursor for DNA biosynthesis.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP.UniRule annotation

Pathwayi: dTTP biosynthesis

This protein is involved in the pathway dTTP biosynthesis, which is part of Pyrimidine metabolism.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway dTTP biosynthesis and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei21dUMPUniRule annotation1
Active sitei192NucleophileUniRule annotation1
Binding sitei2175,10-methylenetetrahydrofolateUniRule annotation1
Binding sitei225dUMPUniRule annotation1
Binding sitei3225,10-methylenetetrahydrofolate; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 173dUMP; shared with dimeric partnerUniRule annotation2
Nucleotide bindingi214 – 217dUMPUniRule annotation4
Nucleotide bindingi255 – 257dUMPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processNucleotide biosynthesis

Enzyme and pathway databases

BioCyciPPUT160488:G1G01-5486-MONOMER
UniPathwayiUPA00575

Names & Taxonomyi

Protein namesi
Recommended name:
Thymidylate synthaseUniRule annotation (EC:2.1.1.45UniRule annotation)
Short name:
TSUniRule annotation
Short name:
TSaseUniRule annotation
Gene namesi
Name:thyAUniRule annotation
Ordered Locus Names:PP_5141
OrganismiPseudomonas putida (strain ATCC 47054 / DSM 6125 / NCIMB 11950 / KT2440)
Taxonomic identifieri160488 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000000556 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001410051 – 323Thymidylate synthaseAdd BLAST323

Proteomic databases

PRIDEiQ88CN9

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi160488.PP_5141

Structurei

3D structure databases

ProteinModelPortaliQ88CN9
SMRiQ88CN9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C0V Bacteria
COG0207 LUCA
HOGENOMiHOG000257900
KOiK00560
OMAiKQYLDLC

Family and domain databases

CDDicd00351 TS_Pyrimidine_HMase, 1 hit
Gene3Di3.30.572.10, 1 hit
HAMAPiMF_00008 Thymidy_synth_bact, 1 hit
InterProiView protein in InterPro
IPR023451 Thymidate_synth/dCMP_Mease
IPR036926 Thymidate_synth/dCMP_Mease_sf
IPR000398 Thymidylate_synthase
PfamiView protein in Pfam
PF00303 Thymidylat_synt, 1 hit
PRINTSiPR00108 THYMDSNTHASE
SUPFAMiSSF55831 SSF55831, 2 hits
TIGRFAMsiTIGR03284 thym_sym, 1 hit

Sequencei

Sequence statusi: Complete.

Q88CN9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQYLDLVRD VIDNGTLQGN RTGIRTISLP GAMLRFDLQK GFPAITTRKL
60 70 80 90 100
AFKSAIGEMV GFLRGVKNAG EFRELGCKVW DQNANENAQW LANPFRQGHD
110 120 130 140 150
DLGEIYGVQW RQWPGYKRIP LSNPAAIEMA EQAGFRRIAQ DEEDGVAFVI
160 170 180 190 200
LYKAIDQVRQ CLDTIANDPG SRRILFHGWN CAQLDEMALP PCHLLYQFHP
210 220 230 240 250
NVETREISLT LYIRSNDLGL GTPFNLTEGA ALLSLFGRLT GYTPRWFTYF
260 270 280 290 300
IGDAHVYENH LDMLNEQLKR EPLEAPKLVI SDRVPAFAET GKYEPEWLEK
310 320
IEPSDFWLEG YEHHAPMTAP MAV
Length:323
Mass (Da):36,772
Last modified:June 1, 2003 - v1
Checksum:i858477C4CD60D3C9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE015451 Genomic DNA Translation: AAN70706.1
RefSeqiNP_747242.1, NC_002947.4
WP_010955677.1, NC_002947.4

Genome annotation databases

EnsemblBacteriaiAAN70706; AAN70706; PP_5141
GeneIDi1042582
KEGGippu:PP_5141
PATRICifig|160488.4.peg.5488

Similar proteinsi

Entry informationi

Entry nameiTYSY_PSEPK
AccessioniPrimary (citable) accession number: Q88CN9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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