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Q88CN9 (TYSY_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:PP_5141
OrganismPseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]
Taxonomic identifier160488 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas

Protein attributes

Sequence length323 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 323323Thymidylate synthase HAMAP-Rule MF_00008
PRO_0000141005

Sites

Active site1921 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q88CN9 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 858477C4CD60D3C9

FASTA32336,772
        10         20         30         40         50         60 
MKQYLDLVRD VIDNGTLQGN RTGIRTISLP GAMLRFDLQK GFPAITTRKL AFKSAIGEMV 

        70         80         90        100        110        120 
GFLRGVKNAG EFRELGCKVW DQNANENAQW LANPFRQGHD DLGEIYGVQW RQWPGYKRIP 

       130        140        150        160        170        180 
LSNPAAIEMA EQAGFRRIAQ DEEDGVAFVI LYKAIDQVRQ CLDTIANDPG SRRILFHGWN 

       190        200        210        220        230        240 
CAQLDEMALP PCHLLYQFHP NVETREISLT LYIRSNDLGL GTPFNLTEGA ALLSLFGRLT 

       250        260        270        280        290        300 
GYTPRWFTYF IGDAHVYENH LDMLNEQLKR EPLEAPKLVI SDRVPAFAET GKYEPEWLEK 

       310        320 
IEPSDFWLEG YEHHAPMTAP MAV 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE015451 Genomic DNA. Translation: AAN70706.1.
RefSeqNP_747242.1. NC_002947.3.

3D structure databases

ProteinModelPortalQ88CN9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160488.PP_5141.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN70706; AAN70706; PP_5141.
GeneID1042582.
KEGGppu:PP_5141.
PATRIC19949118. VBIPsePut30601_5488.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257900.
KOK00560.
OMAFGRQIRY.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycPPUT160488:GIXO-5237-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 2 hits.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTYSY_PSEPK
AccessionPrimary (citable) accession number: Q88CN9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2003
Last modified: May 14, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways