Q88CI9 (GCSP2_PSEPK) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine dehydrogenase [decarboxylating] 2
EC=1.4.4.2

Alternative name(s):
Glycine cleavage system P-protein 2
Glycine decarboxylase 2

Gene names

Name:	gcvP2
Synonyms:	gcvP-2
Ordered Locus Names:	PP_5192

Organism

Pseudomonas putida (strain KT2440) [Complete proteome] [HAMAP]

Taxonomic identifier

160488 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	957 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP MF_00711
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00711
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_00711
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 957	957	Glycine dehydrogenase [decarboxylating] 2 HAMAP MF_00711		PRO_0000166928
Amino acid modifications
	Modified residue	707	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q88CI9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 85277F8806A51C46	FASTA	957	104,038
	10 20 30 40 50 60 MSQSPSLHQL QELNPFLRRH LGPDATEQQA MLNALGIASR NELIEQTVPP DIRLNRPLDL 70 80 90 100 110 120 PAALDEQAAL AKLAGYAEQN QVWTSLIGMG YHGTITPTVI LRNVLENPGW YTAYTPYQPE 130 140 150 160 170 180 IAQGRLEALL NFQQMVIDLT GLPLANASLL DEATAAAEAM ALAKRVARNK SNAFFADEHC 190 200 210 220 230 240 HPQTLSVLKT RAEGFGFELI VDSVDNLAKH SVFGALLQYP DTHGEVRDLR PLIDQLHSQQ 250 260 270 280 290 300 ALACVAADLL SLVVLAPPGE LGADVVLGST QRFGVPMGYG GPHAAYFACR DDYKRAMPGR 310 320 330 340 350 360 IIGVSRDARG NTALRMALQT REQHIRREKA NSNICTAQVL LANIAGFYAV YHGPEGLQRI 370 380 390 400 410 420 AQRVHRLTFI LAAGLEAKGI KRLNQHFFDT LTLNVGGAQA AIIESAEAAH INLRILGRGH 430 440 450 460 470 480 LGVSLDETCT EQTVLRLLDI FLGVDHGLEI TALDQLALPE GIPASLVRRT PFLAHPVFNL 490 500 510 520 530 540 HHSETEMLRY LKQLENKDLA LNQSMIPLGS CTMKLNATSE MIPITWPGFA QLHPFAPAAQ 550 560 570 580 590 600 AAGYKAMIDE LESWLCAITG FDAICMQPNS GAQGEYAGLM AITRYHCSRH QPMRTLCLIP 610 620 630 640 650 660 SSAHGTNPAS AQMAGMEVVI VDCDNDGNVD LADLKAKAHA AGERLSCLMI TYPSTHGVYE 670 680 690 700 710 720 EGIREICDVV HQYGGQVYMD GANLNAQVGL ARPADIGADV SHMNLHKTFC IPHGGGGPGM 730 740 750 760 770 780 GPIGIRAHLK PFVASHPVVP VPGLDPNNSA VSAAPWGSAS ILPISWMYIA MMGPQLADAS 790 800 810 820 830 840 EVAILSANYL ASQLGAAFPV LYRGRNQRVA HECILDLRPL KALTGISEED VAKRLMDYGF 850 860 870 880 890 900 HAPTMSFPVP GTLMVEPTES ESKAELDRFV EAMLAIRAEI DEVQQGNWPA ENNPLKHAPH 910 920 930 940 950 TLADVLGVWD RPYSLEQAVA PSAHVRQHKY WPAVNRVDNV YGDRNLFCAC VPVEAYR

References

[1]

"Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440." Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H., Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M., Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F., Madupu R., Nelson W.C., White O. , Peterson J.D., Khouri H.M., Hance I., Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A., Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H., Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C., Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M. Environ. Microbiol. 4:799-808(2002) [PubMed: 12534463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KT2440.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE015451 Genomic DNA. Translation: AAN70757.1.
RefSeq	NP_747293.1. NC_002947.3.
3D structure databases
ProteinModelPortal	Q88CI9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q88CI9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1042488.
GenomeReviews	Gene locus PP_5192 in contig AE015451_GR.
KEGG	ppu:PP_5192.
NMPDR	fig|160488.1.peg.5123.
PATRIC	19949222. VBIPsePut30601_5540.
TIGR	PP_5192.
Phylogenomic databases
eggNOG	COG1003.
HOGENOM	HBG286723.
OMA	AMMGPQL.
PhylomeDB	Q88CI9.
ProtClustDB	PRK12566.
Enzyme and pathway databases
BioCyc	PPUT160488:PP_5192-MONOMER.
Family and domain databases
HAMAP	MF_00711. GcvP. [Tree]
InterPro	IPR018247. EF_Hand_1_Ca_BS. IPR020580. GDC-P_N. IPR020581. GDC_P. IPR003437. GDC_P_homo. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 2 hits.
KO	K00281.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 2 hits. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 2 hits.
TIGRFAMs	TIGR00461. GcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP2_PSEPK

Accession

Primary (citable) accession number: Q88CI9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program